FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1588051751> ?p ?o ?g. }

• W1588051751 endingPage "46" @default.
• W1588051751 startingPage "34" @default.
• W1588051751 abstract "Because of their small size and density, umbilical cord blood (UCB)-derived very small embryonic/epiblast-like stem cells (VSELs) are usually lost at various steps of UCB preparation. Accordingly, we noticed that a significant number of these cells, which are smaller than erythrocytes, are lost during gradient centrifugation over Ficoll-Paque as well as during routine volume depletion of UCB units before freezing. To preserve these cells in final UCB preparations, we propose a relatively short and economical three-step isolation protocol that allows recovery of approximately 60% of the initial number of Lin−/CD45−/CD133+ UCB-VSELs present in freshly harvested UCB units. In this novel approach (i) UCB is lysed in a hypotonic ammonium chloride solution to deplete erythrocytes; (ii) CD133+ including VSELs cells are enriched by employing immunomagnetic beads; and subsequently (iii) Lin−/CD45−/CD133+ cells are sorted by fluorescence-activated cell sorting. The whole isolation procedure takes approximately 2–3 h per UCB unit and isolated cells are highly enriched for an Oct-4+ and SSEA-4+ population of small Lin−/CD45−/CD133+ cells." @default.
• W1588051751 created "2016-06-24" @default.
• W1588051751 creator A5000979061 @default.
• W1588051751 creator A5026426787 @default.
• W1588051751 creator A5059307574 @default.
• W1588051751 creator A5059797628 @default.
• W1588051751 creator A5060515541 @default.
• W1588051751 creator A5089545311 @default.
• W1588051751 date "2010-01-01" @default.
• W1588051751 modified "2023-10-15" @default.
• W1588051751 title "Optimization of isolation and further characterization of umbilical cord blood-derived very small embryonic/ epiblast-like stem cells (VSELs)" @default.
• W1588051751 cites W1542326554 @default.
• W1588051751 cites W1621991227 @default.
• W1588051751 cites W1962031413 @default.
• W1588051751 cites W1966100365 @default.
• W1588051751 cites W1969986792 @default.
• W1588051751 cites W1970949498 @default.
• W1588051751 cites W1975373424 @default.
• W1588051751 cites W1982961252 @default.
• W1588051751 cites W1984270581 @default.
• W1588051751 cites W1989378631 @default.
• W1588051751 cites W1991374176 @default.
• W1588051751 cites W2006147204 @default.
• W1588051751 cites W2006749692 @default.
• W1588051751 cites W2014648588 @default.
• W1588051751 cites W2017418598 @default.
• W1588051751 cites W2021482268 @default.
• W1588051751 cites W2035529239 @default.
• W1588051751 cites W2042686954 @default.
• W1588051751 cites W2047321579 @default.
• W1588051751 cites W2065767970 @default.
• W1588051751 cites W2073032682 @default.
• W1588051751 cites W2090041902 @default.
• W1588051751 cites W2094040645 @default.
• W1588051751 cites W2097834438 @default.
• W1588051751 cites W2109210816 @default.
• W1588051751 cites W2126179536 @default.
• W1588051751 cites W2129017319 @default.
• W1588051751 cites W2147164277 @default.
• W1588051751 cites W2152168811 @default.
• W1588051751 cites W2162647147 @default.
• W1588051751 cites W2166741387 @default.
• W1588051751 cites W2328808046 @default.
• W1588051751 doi "https://doi.org/10.1111/j.1600-0609.2009.01352.x" @default.
• W1588051751 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19758351" @default.
• W1588051751 hasPublicationYear "2010" @default.
• W1588051751 type Work @default.
• W1588051751 sameAs 1588051751 @default.
• W1588051751 citedByCount "52" @default.
• W1588051751 countsByYear W15880517512012 @default.
• W1588051751 countsByYear W15880517512013 @default.
• W1588051751 countsByYear W15880517512014 @default.
• W1588051751 countsByYear W15880517512015 @default.
• W1588051751 countsByYear W15880517512016 @default.
• W1588051751 countsByYear W15880517512017 @default.
• W1588051751 countsByYear W15880517512018 @default.
• W1588051751 countsByYear W15880517512020 @default.
• W1588051751 countsByYear W15880517512021 @default.
• W1588051751 countsByYear W15880517512022 @default.
• W1588051751 crossrefType "journal-article" @default.
• W1588051751 hasAuthorship W1588051751A5000979061 @default.
• W1588051751 hasAuthorship W1588051751A5026426787 @default.
• W1588051751 hasAuthorship W1588051751A5059307574 @default.
• W1588051751 hasAuthorship W1588051751A5059797628 @default.
• W1588051751 hasAuthorship W1588051751A5060515541 @default.
• W1588051751 hasAuthorship W1588051751A5089545311 @default.
• W1588051751 hasConcept C104317684 @default.
• W1588051751 hasConcept C129536746 @default.
• W1588051751 hasConcept C137061746 @default.
• W1588051751 hasConcept C145103041 @default.
• W1588051751 hasConcept C153911025 @default.
• W1588051751 hasConcept C16685009 @default.
• W1588051751 hasConcept C185592680 @default.
• W1588051751 hasConcept C202751555 @default.
• W1588051751 hasConcept C203014093 @default.
• W1588051751 hasConcept C2776955114 @default.
• W1588051751 hasConcept C2778630268 @default.
• W1588051751 hasConcept C28328180 @default.
• W1588051751 hasConcept C2908647359 @default.
• W1588051751 hasConcept C5362183 @default.
• W1588051751 hasConcept C553184892 @default.
• W1588051751 hasConcept C55493867 @default.
• W1588051751 hasConcept C71924100 @default.
• W1588051751 hasConcept C75059768 @default.
• W1588051751 hasConcept C86803240 @default.
• W1588051751 hasConcept C95444343 @default.
• W1588051751 hasConcept C99454951 @default.
• W1588051751 hasConceptScore W1588051751C104317684 @default.
• W1588051751 hasConceptScore W1588051751C129536746 @default.
• W1588051751 hasConceptScore W1588051751C137061746 @default.
• W1588051751 hasConceptScore W1588051751C145103041 @default.
• W1588051751 hasConceptScore W1588051751C153911025 @default.
• W1588051751 hasConceptScore W1588051751C16685009 @default.
• W1588051751 hasConceptScore W1588051751C185592680 @default.
• W1588051751 hasConceptScore W1588051751C202751555 @default.
• W1588051751 hasConceptScore W1588051751C203014093 @default.
• W1588051751 hasConceptScore W1588051751C2776955114 @default.
• W1588051751 hasConceptScore W1588051751C2778630268 @default.

• next