FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1588178383> ?p ?o ?g. }

• W1588178383 endingPage "42" @default.
• W1588178383 startingPage "4134" @default.
• W1588178383 abstract "Flavonols are produced by the desaturation of flavanols catalyzed by flavonol synthase. The enzyme belongs to the class of intermolecular dioxygenases which depend on molecular oxygen and FeII/2-oxoglutarate for activity, and have been in focus of structural studies recently. Flavonol synthase cDNAs were cloned from six plant species, but none of the enzymes had been studied in detail. Therefore, a cDNA from Citrus unshiu (Satsuma mandarin) designated as flavonol synthase was expressed in Escherichia coli, and the purified recombinant enzyme was subjected to kinetic and mutational chacterizations. The integrity of the recombinant synthase was revealed by a molecular ion from MALDI-TOF mass spectrometry at m/z 37888 +/- 40 (as compared to 37899 Da calculated for the translated polypeptide), and by partial N-terminal sequencing. Maximal flavonol synthase activity was observed in the range of pH 5-6 with dihydroquercetin as substrate and a temperature optimum at about 37 degrees C. Km values of 272, 11 and 36 micro m were determined for dihydroquercetin, FeII and 2-oxoglutarate, respectively, with a sixfold higher affinity to dihydrokaempferol (Km 45 micro m). Flavonol synthase polypeptides share an overall sequence similarity of 85% (47% identity), whereas only 30-60% similarity were apparent with other dioxygenases. Like the other dioxygenases of this class, Citrus flavonol synthase cDNA encodes eight strictly conserved amino-acid residues which include two histidines (His221, His277) and one acidic amino acid (Asp223) residue for FeII-coordination, an arginine (Arg287) proposed to bind 2-oxoglutarate, and four amino acids (Gly68, His75, Gly261, Pro207) with no obvious functionality. Replacements of Gly68 and Gly261 by alanine reduced the catalytic activity by 95%, while the exchange of these Gly residues for proline completely abolished the enzyme activity. Alternatively, the substitution of Pro207 by glycine hardly affected the activity. The data suggest that Gly68 and Gly261, at least, are required for proper folding of the flavonol synthase polypeptide." @default.
• W1588178383 created "2016-06-24" @default.
• W1588178383 creator A5002785136 @default.
• W1588178383 creator A5009142992 @default.
• W1588178383 creator A5037475095 @default.
• W1588178383 creator A5046554817 @default.
• W1588178383 creator A5080803287 @default.
• W1588178383 creator A5090831690 @default.
• W1588178383 date "2002-08-01" @default.
• W1588178383 modified "2023-09-23" @default.
• W1588178383 title "Functional expression and mutational analysis of flavonol synthase from Citrus unshiu." @default.
• W1588178383 cites W1520097912 @default.
• W1588178383 cites W1531753473 @default.
• W1588178383 cites W1650854146 @default.
• W1588178383 cites W1794609748 @default.
• W1588178383 cites W1915092483 @default.
• W1588178383 cites W1991721460 @default.
• W1588178383 cites W1998945596 @default.
• W1588178383 cites W2001525071 @default.
• W1588178383 cites W2007243697 @default.
• W1588178383 cites W2009646062 @default.
• W1588178383 cites W2025453448 @default.
• W1588178383 cites W2025902647 @default.
• W1588178383 cites W2037115606 @default.
• W1588178383 cites W2037157646 @default.
• W1588178383 cites W2045315686 @default.
• W1588178383 cites W2046754269 @default.
• W1588178383 cites W2047590061 @default.
• W1588178383 cites W2050494814 @default.
• W1588178383 cites W2056816046 @default.
• W1588178383 cites W2057222541 @default.
• W1588178383 cites W2063568362 @default.
• W1588178383 cites W2067864225 @default.
• W1588178383 cites W2087183715 @default.
• W1588178383 cites W2090784961 @default.
• W1588178383 cites W2092084889 @default.
• W1588178383 cites W2095788149 @default.
• W1588178383 cites W2100837269 @default.
• W1588178383 cites W2101108802 @default.
• W1588178383 cites W2104497702 @default.
• W1588178383 cites W2120145238 @default.
• W1588178383 cites W2120174947 @default.
• W1588178383 cites W2120302902 @default.
• W1588178383 cites W2122967180 @default.
• W1588178383 cites W2134823451 @default.
• W1588178383 cites W2138270253 @default.
• W1588178383 cites W2147788330 @default.
• W1588178383 cites W2238990854 @default.
• W1588178383 cites W2245390538 @default.
• W1588178383 cites W2290003007 @default.
• W1588178383 cites W2338151994 @default.
• W1588178383 cites W2888511473 @default.
• W1588178383 doi "https://doi.org/10.1046/j.1432-1033.2002.03108.x" @default.
• W1588178383 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12180990" @default.
• W1588178383 hasPublicationYear "2002" @default.
• W1588178383 type Work @default.
• W1588178383 sameAs 1588178383 @default.
• W1588178383 citedByCount "68" @default.
• W1588178383 countsByYear W15881783832012 @default.
• W1588178383 countsByYear W15881783832013 @default.
• W1588178383 countsByYear W15881783832014 @default.
• W1588178383 countsByYear W15881783832015 @default.
• W1588178383 countsByYear W15881783832016 @default.
• W1588178383 countsByYear W15881783832017 @default.
• W1588178383 countsByYear W15881783832018 @default.
• W1588178383 countsByYear W15881783832019 @default.
• W1588178383 countsByYear W15881783832020 @default.
• W1588178383 countsByYear W15881783832021 @default.
• W1588178383 countsByYear W15881783832022 @default.
• W1588178383 countsByYear W15881783832023 @default.
• W1588178383 crossrefType "journal-article" @default.
• W1588178383 hasAuthorship W1588178383A5002785136 @default.
• W1588178383 hasAuthorship W1588178383A5009142992 @default.
• W1588178383 hasAuthorship W1588178383A5037475095 @default.
• W1588178383 hasAuthorship W1588178383A5046554817 @default.
• W1588178383 hasAuthorship W1588178383A5080803287 @default.
• W1588178383 hasAuthorship W1588178383A5090831690 @default.
• W1588178383 hasBestOaLocation W15881783831 @default.
• W1588178383 hasConcept C104317684 @default.
• W1588178383 hasConcept C112243037 @default.
• W1588178383 hasConcept C167625842 @default.
• W1588178383 hasConcept C181199279 @default.
• W1588178383 hasConcept C185592680 @default.
• W1588178383 hasConcept C187882448 @default.
• W1588178383 hasConcept C2776835245 @default.
• W1588178383 hasConcept C515207424 @default.
• W1588178383 hasConcept C55493867 @default.
• W1588178383 hasConcept C58121356 @default.
• W1588178383 hasConcept C59822182 @default.
• W1588178383 hasConcept C86803240 @default.
• W1588178383 hasConceptScore W1588178383C104317684 @default.
• W1588178383 hasConceptScore W1588178383C112243037 @default.
• W1588178383 hasConceptScore W1588178383C167625842 @default.
• W1588178383 hasConceptScore W1588178383C181199279 @default.
• W1588178383 hasConceptScore W1588178383C185592680 @default.
• W1588178383 hasConceptScore W1588178383C187882448 @default.
• W1588178383 hasConceptScore W1588178383C2776835245 @default.
• W1588178383 hasConceptScore W1588178383C515207424 @default.

• next