Matches in SemOpenAlex for { <https://semopenalex.org/work/W1588768890> ?p ?o ?g. }
- W1588768890 endingPage "1009" @default.
- W1588768890 startingPage "1002" @default.
- W1588768890 abstract "The RNA species synthesized in vitro by a transcribing nucleoprotein (TNP) complex of vesicular stomatitis virus (VSV) were translated with high efficiency in a fractionated cell-free system derived from reticulocytes. The use of TNP complexes isolated from VSV Indiana, VSV New Jersey, and Chandipura viruses showed that in each case the predominant polypeptides synthesized had electrophoretic mobilities identical to their virion N, NS, and M polypeptides in proportions reflecting those found in infected cells rather than purified virions. A minor polypeptide corresponding to unglycosylated polypeptide G was also observed, but the in vitro synthesis of polypeptide L was not detected. The addition of RNase inhibitor to transcription mixtures markedly increased the rate of RNA synthesis. Furthermore, the messenger activity of the RNA was significantly enhanced. The inclusion of S-adenosyl L-methionine during transcription substantially increased the messenger activity of the product RNA, suggesting a requirement for methylation. Fractionation by oligodeoxythymidylic acid-cellulose chromatography revealed that the RNA required a polyadnylic acid tract for messenger activity." @default.
- W1588768890 created "2016-06-24" @default.
- W1588768890 creator A5040394865 @default.
- W1588768890 creator A5075609873 @default.
- W1588768890 date "1977-03-01" @default.
- W1588768890 modified "2023-10-12" @default.
- W1588768890 title "Cell-free translation of RNA synthesized in vitro by a transcribing nucleoprotein complex prepared from purified vesicular stomatitis virus" @default.
- W1588768890 cites W1484147472 @default.
- W1588768890 cites W1499962811 @default.
- W1588768890 cites W1545990783 @default.
- W1588768890 cites W1578428334 @default.
- W1588768890 cites W1578456382 @default.
- W1588768890 cites W1578557334 @default.
- W1588768890 cites W1583733441 @default.
- W1588768890 cites W1584168065 @default.
- W1588768890 cites W1825846957 @default.
- W1588768890 cites W1969817011 @default.
- W1588768890 cites W1970537831 @default.
- W1588768890 cites W1972398797 @default.
- W1588768890 cites W1975751316 @default.
- W1588768890 cites W1977242365 @default.
- W1588768890 cites W1985517822 @default.
- W1588768890 cites W1985728727 @default.
- W1588768890 cites W1995556647 @default.
- W1588768890 cites W2012951194 @default.
- W1588768890 cites W2025091176 @default.
- W1588768890 cites W2030999163 @default.
- W1588768890 cites W2034277598 @default.
- W1588768890 cites W2056975885 @default.
- W1588768890 cites W2059924977 @default.
- W1588768890 cites W2072644415 @default.
- W1588768890 cites W2119465766 @default.
- W1588768890 cites W2166239756 @default.
- W1588768890 doi "https://doi.org/10.1128/jvi.21.3.1002-1009.1977" @default.
- W1588768890 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/515640" @default.
- W1588768890 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/191633" @default.
- W1588768890 hasPublicationYear "1977" @default.
- W1588768890 type Work @default.
- W1588768890 sameAs 1588768890 @default.
- W1588768890 citedByCount "15" @default.
- W1588768890 crossrefType "journal-article" @default.
- W1588768890 hasAuthorship W1588768890A5040394865 @default.
- W1588768890 hasAuthorship W1588768890A5075609873 @default.
- W1588768890 hasBestOaLocation W15887688901 @default.
- W1588768890 hasConcept C10419127 @default.
- W1588768890 hasConcept C104317684 @default.
- W1588768890 hasConcept C105580179 @default.
- W1588768890 hasConcept C138885662 @default.
- W1588768890 hasConcept C153911025 @default.
- W1588768890 hasConcept C159047783 @default.
- W1588768890 hasConcept C162092063 @default.
- W1588768890 hasConcept C179926584 @default.
- W1588768890 hasConcept C202751555 @default.
- W1588768890 hasConcept C2522874641 @default.
- W1588768890 hasConcept C2777974785 @default.
- W1588768890 hasConcept C2779057330 @default.
- W1588768890 hasConcept C2909090456 @default.
- W1588768890 hasConcept C2910348395 @default.
- W1588768890 hasConcept C3675279 @default.
- W1588768890 hasConcept C41258723 @default.
- W1588768890 hasConcept C41895202 @default.
- W1588768890 hasConcept C552990157 @default.
- W1588768890 hasConcept C55493867 @default.
- W1588768890 hasConcept C60325843 @default.
- W1588768890 hasConcept C67705224 @default.
- W1588768890 hasConcept C86803240 @default.
- W1588768890 hasConceptScore W1588768890C10419127 @default.
- W1588768890 hasConceptScore W1588768890C104317684 @default.
- W1588768890 hasConceptScore W1588768890C105580179 @default.
- W1588768890 hasConceptScore W1588768890C138885662 @default.
- W1588768890 hasConceptScore W1588768890C153911025 @default.
- W1588768890 hasConceptScore W1588768890C159047783 @default.
- W1588768890 hasConceptScore W1588768890C162092063 @default.
- W1588768890 hasConceptScore W1588768890C179926584 @default.
- W1588768890 hasConceptScore W1588768890C202751555 @default.
- W1588768890 hasConceptScore W1588768890C2522874641 @default.
- W1588768890 hasConceptScore W1588768890C2777974785 @default.
- W1588768890 hasConceptScore W1588768890C2779057330 @default.
- W1588768890 hasConceptScore W1588768890C2909090456 @default.
- W1588768890 hasConceptScore W1588768890C2910348395 @default.
- W1588768890 hasConceptScore W1588768890C3675279 @default.
- W1588768890 hasConceptScore W1588768890C41258723 @default.
- W1588768890 hasConceptScore W1588768890C41895202 @default.
- W1588768890 hasConceptScore W1588768890C552990157 @default.
- W1588768890 hasConceptScore W1588768890C55493867 @default.
- W1588768890 hasConceptScore W1588768890C60325843 @default.
- W1588768890 hasConceptScore W1588768890C67705224 @default.
- W1588768890 hasConceptScore W1588768890C86803240 @default.
- W1588768890 hasIssue "3" @default.
- W1588768890 hasLocation W15887688901 @default.
- W1588768890 hasLocation W15887688902 @default.
- W1588768890 hasLocation W15887688903 @default.
- W1588768890 hasOpenAccess W1588768890 @default.
- W1588768890 hasPrimaryLocation W15887688901 @default.
- W1588768890 hasRelatedWork W1588768890 @default.
- W1588768890 hasRelatedWork W1601138682 @default.
- W1588768890 hasRelatedWork W1908127430 @default.
- W1588768890 hasRelatedWork W1962557002 @default.