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- W1589289784 abstract "Database mining, followed by heterologous expression and activity confirmation, extended the spectrum of known nitrilases and cyanide hydratases. Their sources were partly eubacteria and archaea but mainly filamentous fungi. This method enabled to obtained enzymes difficult to induce in wild-type strains. Clustering of the sequences and analysis of their specific regions made it possible to predict the substrate specificities of the enzymes. The enzymes obtained in this way proved useful, for instance, in the transformations of arylacetonitriles (mandelonitrile, o-chloromandelonitrile) or in the elimination of inorganic cyanide from wastewaters." @default.
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- W1589289784 date "2014-06-27" @default.
- W1589289784 modified "2023-10-17" @default.
- W1589289784 title "Mining Genomes for Nitrilases" @default.
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- W1589289784 doi "https://doi.org/10.1002/9783527682492.ch12" @default.
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