Matches in SemOpenAlex for { <https://semopenalex.org/work/W1589989788> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W1589989788 endingPage "130" @default.
- W1589989788 startingPage "127" @default.
- W1589989788 abstract "A rapid colorimetric technique for in vitro quantitation of Legionella pneumophila intracellular proliferation in macrophages is described. The assay is based on the electron transport activity of metabolically active L. pneumophila. The yellow tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is cleaved by the mitochondrial activity of viable L. pneumophila, forming a dark formazan derivative with an absorption spectrum different from that of the native compound. The MTT method for measuring intracellular growth of L. pneumophila closely correlated with the CFU assay. The ability of macrophages from the A/J mouse strain to support intracellular growth of L. pneumophila and the ability of desferrioxamine to restrict L. pneumophila intracellular proliferation were confirmed by both methods. The MTT assay offers the advantages of rapidity, simplicity, and cost efficiency over the CFU assay, since it can be performed in the same flat-bottom microtiter plate with measurement in an enzyme-linked immunosorbent assay reader, allowing efficient processing of large numbers of samples." @default.
- W1589989788 created "2016-06-24" @default.
- W1589989788 creator A5011110678 @default.
- W1589989788 creator A5013871861 @default.
- W1589989788 creator A5032965517 @default.
- W1589989788 creator A5065267526 @default.
- W1589989788 creator A5072207657 @default.
- W1589989788 date "1994-01-01" @default.
- W1589989788 modified "2023-09-27" @default.
- W1589989788 title "A rapid colorimetric assay for evaluating Legionella pneumophila growth in macrophages in vitro" @default.
- W1589989788 cites W1949287816 @default.
- W1589989788 cites W1963824793 @default.
- W1589989788 cites W1988154318 @default.
- W1589989788 cites W2022386628 @default.
- W1589989788 cites W2039999383 @default.
- W1589989788 cites W2090979926 @default.
- W1589989788 cites W2114918609 @default.
- W1589989788 cites W2167015312 @default.
- W1589989788 cites W2168977023 @default.
- W1589989788 cites W2406965457 @default.
- W1589989788 doi "https://doi.org/10.1128/jcm.32.1.127-130.1994" @default.
- W1589989788 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/262981" @default.
- W1589989788 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8126166" @default.
- W1589989788 hasPublicationYear "1994" @default.
- W1589989788 type Work @default.
- W1589989788 sameAs 1589989788 @default.
- W1589989788 citedByCount "7" @default.
- W1589989788 countsByYear W15899897882014 @default.
- W1589989788 crossrefType "journal-article" @default.
- W1589989788 hasAuthorship W1589989788A5011110678 @default.
- W1589989788 hasAuthorship W1589989788A5013871861 @default.
- W1589989788 hasAuthorship W1589989788A5032965517 @default.
- W1589989788 hasAuthorship W1589989788A5065267526 @default.
- W1589989788 hasAuthorship W1589989788A5072207657 @default.
- W1589989788 hasBestOaLocation W15899897881 @default.
- W1589989788 hasConcept C153911025 @default.
- W1589989788 hasConcept C185592680 @default.
- W1589989788 hasConcept C202751555 @default.
- W1589989788 hasConcept C2776118409 @default.
- W1589989788 hasConcept C2776892249 @default.
- W1589989788 hasConcept C2779164008 @default.
- W1589989788 hasConcept C2780295090 @default.
- W1589989788 hasConcept C2780783641 @default.
- W1589989788 hasConcept C43617362 @default.
- W1589989788 hasConcept C523546767 @default.
- W1589989788 hasConcept C54355233 @default.
- W1589989788 hasConcept C55493867 @default.
- W1589989788 hasConcept C64299311 @default.
- W1589989788 hasConcept C79879829 @default.
- W1589989788 hasConcept C86803240 @default.
- W1589989788 hasConcept C89423630 @default.
- W1589989788 hasConceptScore W1589989788C153911025 @default.
- W1589989788 hasConceptScore W1589989788C185592680 @default.
- W1589989788 hasConceptScore W1589989788C202751555 @default.
- W1589989788 hasConceptScore W1589989788C2776118409 @default.
- W1589989788 hasConceptScore W1589989788C2776892249 @default.
- W1589989788 hasConceptScore W1589989788C2779164008 @default.
- W1589989788 hasConceptScore W1589989788C2780295090 @default.
- W1589989788 hasConceptScore W1589989788C2780783641 @default.
- W1589989788 hasConceptScore W1589989788C43617362 @default.
- W1589989788 hasConceptScore W1589989788C523546767 @default.
- W1589989788 hasConceptScore W1589989788C54355233 @default.
- W1589989788 hasConceptScore W1589989788C55493867 @default.
- W1589989788 hasConceptScore W1589989788C64299311 @default.
- W1589989788 hasConceptScore W1589989788C79879829 @default.
- W1589989788 hasConceptScore W1589989788C86803240 @default.
- W1589989788 hasConceptScore W1589989788C89423630 @default.
- W1589989788 hasIssue "1" @default.
- W1589989788 hasLocation W15899897881 @default.
- W1589989788 hasLocation W15899897882 @default.
- W1589989788 hasLocation W15899897883 @default.
- W1589989788 hasOpenAccess W1589989788 @default.
- W1589989788 hasPrimaryLocation W15899897881 @default.
- W1589989788 hasRelatedWork W1589989788 @default.
- W1589989788 hasRelatedWork W1923100104 @default.
- W1589989788 hasRelatedWork W1978494873 @default.
- W1589989788 hasRelatedWork W2027519905 @default.
- W1589989788 hasRelatedWork W2072903220 @default.
- W1589989788 hasRelatedWork W2096746799 @default.
- W1589989788 hasRelatedWork W2097202445 @default.
- W1589989788 hasRelatedWork W2153041867 @default.
- W1589989788 hasRelatedWork W2166618324 @default.
- W1589989788 hasRelatedWork W2767727320 @default.
- W1589989788 hasVolume "32" @default.
- W1589989788 isParatext "false" @default.
- W1589989788 isRetracted "false" @default.
- W1589989788 magId "1589989788" @default.
- W1589989788 workType "article" @default.