Matches in SemOpenAlex for { <https://semopenalex.org/work/W1590152938> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W1590152938 endingPage "14092" @default.
- W1590152938 startingPage "14087" @default.
- W1590152938 abstract "1-Bromopinacolone, BrPin, acts initially as a reversible competitive inhibitor for acetylcholinesterase, KI = 0.18 mM in hydrolysis of acetylcholine. Unlike bromoacetone, with time it acts as an irreversible covalent inhibitor. BrPin has a hydrolytic half-life of 30 h at the pH of incubation, 7.8. The enzyme-BrPin complex is 50% inactivated in 2 h. First order kinetics are observed; the rate constant is proportional to the concentration of complex. Retardation by cationic inhibitors of the inactivation is consistent with inactivation occurring as a result of binding of BrPin to the active site. Efficiency of irreversible inhibition by BrPin is essentially the same for hydrolysis of cationic and uncharged substrates, acetylcholine, 3,3-dimethylbutyl acetate, phenyl acetate, n-butyl acetate, and indophenyl acetate. In contrast, a cationic alkylating agent, N,N-dimethyl-2-phenylaziridinium ion, DPA, acts noncompetitively; it inactivates completely toward cationic, and partially toward uncharged substrates, and does so slightly more rapidly than BrPin, but less than would be commensurate with its greater intrinsic reactivity. Enzyme first treated with DPA is inactivated by BrPin toward hydrolysis of 3,3-dimethylbutyl acetate. It is proposed that BrPin, and not DPA, binds and reacts in, and may be a useful labeling agent for, the active site." @default.
- W1590152938 created "2016-06-24" @default.
- W1590152938 creator A5025499400 @default.
- W1590152938 creator A5046140947 @default.
- W1590152938 creator A5066540747 @default.
- W1590152938 creator A5088822238 @default.
- W1590152938 date "1982-12-01" @default.
- W1590152938 modified "2023-09-29" @default.
- W1590152938 title "1-Bromopinacolone, an active site-directed covalent inhibitor for acetylcholinesterase." @default.
- W1590152938 cites W132477964 @default.
- W1590152938 cites W1555252779 @default.
- W1590152938 cites W1567765570 @default.
- W1590152938 cites W1967450262 @default.
- W1590152938 cites W1999258515 @default.
- W1590152938 cites W2000477613 @default.
- W1590152938 cites W2004478639 @default.
- W1590152938 cites W2015887024 @default.
- W1590152938 cites W2040095977 @default.
- W1590152938 cites W204138135 @default.
- W1590152938 cites W2044562660 @default.
- W1590152938 cites W2061091446 @default.
- W1590152938 cites W2063413595 @default.
- W1590152938 cites W2078683959 @default.
- W1590152938 cites W2087864862 @default.
- W1590152938 cites W234262503 @default.
- W1590152938 cites W68267517 @default.
- W1590152938 cites W969708734 @default.
- W1590152938 cites W988479680 @default.
- W1590152938 doi "https://doi.org/10.1016/s0021-9258(19)45347-7" @default.
- W1590152938 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7142196" @default.
- W1590152938 hasPublicationYear "1982" @default.
- W1590152938 type Work @default.
- W1590152938 sameAs 1590152938 @default.
- W1590152938 citedByCount "16" @default.
- W1590152938 countsByYear W15901529382014 @default.
- W1590152938 countsByYear W15901529382016 @default.
- W1590152938 crossrefType "journal-article" @default.
- W1590152938 hasAuthorship W1590152938A5025499400 @default.
- W1590152938 hasAuthorship W1590152938A5046140947 @default.
- W1590152938 hasAuthorship W1590152938A5066540747 @default.
- W1590152938 hasAuthorship W1590152938A5088822238 @default.
- W1590152938 hasBestOaLocation W15901529381 @default.
- W1590152938 hasConcept C178790620 @default.
- W1590152938 hasConcept C180577832 @default.
- W1590152938 hasConcept C181199279 @default.
- W1590152938 hasConcept C185592680 @default.
- W1590152938 hasConcept C2777544248 @default.
- W1590152938 hasConcept C2778816929 @default.
- W1590152938 hasConcept C41183919 @default.
- W1590152938 hasConcept C55493867 @default.
- W1590152938 hasConcept C71240020 @default.
- W1590152938 hasConceptScore W1590152938C178790620 @default.
- W1590152938 hasConceptScore W1590152938C180577832 @default.
- W1590152938 hasConceptScore W1590152938C181199279 @default.
- W1590152938 hasConceptScore W1590152938C185592680 @default.
- W1590152938 hasConceptScore W1590152938C2777544248 @default.
- W1590152938 hasConceptScore W1590152938C2778816929 @default.
- W1590152938 hasConceptScore W1590152938C41183919 @default.
- W1590152938 hasConceptScore W1590152938C55493867 @default.
- W1590152938 hasConceptScore W1590152938C71240020 @default.
- W1590152938 hasIssue "23" @default.
- W1590152938 hasLocation W15901529381 @default.
- W1590152938 hasOpenAccess W1590152938 @default.
- W1590152938 hasPrimaryLocation W15901529381 @default.
- W1590152938 hasRelatedWork W2021794406 @default.
- W1590152938 hasRelatedWork W2041365488 @default.
- W1590152938 hasRelatedWork W2079120543 @default.
- W1590152938 hasRelatedWork W2158533759 @default.
- W1590152938 hasRelatedWork W3014134162 @default.
- W1590152938 hasRelatedWork W3108516116 @default.
- W1590152938 hasRelatedWork W4297902663 @default.
- W1590152938 hasRelatedWork W4309518821 @default.
- W1590152938 hasRelatedWork W1825518933 @default.
- W1590152938 hasRelatedWork W184245734 @default.
- W1590152938 hasVolume "257" @default.
- W1590152938 isParatext "false" @default.
- W1590152938 isRetracted "false" @default.
- W1590152938 magId "1590152938" @default.
- W1590152938 workType "article" @default.