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• W1591044313 abstract "In the present study a pancreatic tissue slice preparation was established to enable the characterization of insulin secreting β-cells in close to in vivo conditions. The procedure to obtain pancreatic tissue slices was found to be rapid and easily reproducible. Slices were viable for at least one day and gross morphology was well preserved. Functionality of β-cells in tissue slices was confirmed by measuring released insulin after application of high glucose concentrations. The tissue slice preparation enabled the execution of electrophysiological experiments on β-cells located in deeper layers of the islet of Langerhans. Characterizing β-cells in tissue slices revealed several different properties compared to preparations used to date. First, the secretion rate acquired by monitoring capacitance in β-cells of tissue slices was comparable to biochemical measurements of insulin release obtained from perfused pancreas. Experiments on cultured β-cells and β-cells in isolated islets of Langerhans failed to show this similarity. Furthermore, in tissue slices KATP channels in β-cells showed decreased sensitivity to ATP in comparison to dispersed β-cells, shifting the regulation of KATP channels by ATP to physiological levels. Additionally, the run-down of KATP channels in β-cells of tissue slices was shown to be accelerated. Recording electrical activity in response to stimulating agents from β-cells in slices showed the characteristic bursting pattern. However, studies on gap junction-deficient mice (Cx36 ko) proved electrical activity of β-cells to vanish due to wash-out after dialysis of the pipette solution. The electrical activity recorded from wild type mice in whole-cell configuration reflected the activity of electrically coupled β-cells. Furthermore, the presence of gap junctions prevented electrical activity in a single stimulated β-cell, as long as the majority of coupled cells was still in a resting state, contributing to synchronized insulin release from the islet. The study showed pancreatic tissue slices as a promising, less invasive method to study the function of β-cells. In this preparation β-cells were observed to exhibit properties assumed for in vivo conditions. Employment of the pancreatic tissue slice preparation will serve as a suitable system to gain further insight into the complex interactions controlling blood glucose homeostasis." @default.
• W1591044313 created "2016-06-24" @default.
• W1591044313 creator A5030052716 @default.
• W1591044313 date "2022-02-20" @default.
• W1591044313 modified "2023-09-30" @default.
• W1591044313 title "Electrophysiological characterization of insulin secreting beta-cells in pancreatic tissue slices" @default.
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