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- W1591567459 abstract "Abstract Binding of concanavalin A (conA) to human peripheral blood lymphocytes or to lymphoblasts is inhibited by α-D-mannopyranosides or α-D-glucopyranosides. After 1 hr incubation of cells with con A over 80% of the bound con A can be washed off with methyl-α-D-mannoside; after 24 hr only about 25% of the cell associated con A can be removed by competitor. This sequestration is temperature dependent. Cells which have been treated with con A for 20 hr still have as many combining sites for freshly added con A as do non-pretreated cells, even though about 75% of the previously added con A remains associated with the cells. Con A stimulated cells can be separated from non-stimulated cells on Ficoll gradients. Irreversibly bound con A is preferentially accumulated in the stimulated cells. However, this irreversibly bound con A does not stimulate. We suggest that irreversibly bound con A is inside the cell and that internalization is a result of the early activation of energy generating reactions by the mitogenic stimulus. The stimulatory effect depends on reactions at the cell surface." @default.
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- W1591567459 date "1973-08-01" @default.
- W1591567459 modified "2023-10-17" @default.
- W1591567459 title "Proliferation of Stimulated Human Peripheral Blood Lymphocytes: Preferential Incorporation of Concanavalin a by Stimulated Cells and Mitogenic Activity" @default.
- W1591567459 doi "https://doi.org/10.4049/jimmunol.111.2.424" @default.
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