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- W1591567774 abstract "The objective of this study was to optimize the condition of the Agrobacterium mediated alfalfa transformation so that the transformation plants can be obtained. With alfalfa hypocotyls as explants, the factors affecting the transformation frequency (preculture time, concentration of Agrobacterium and antibiotics) were studied. High transformation rate was gained when alfalfa hypocotyls precultured for 5d were immersed into the OD600 0. 5 Agrobacterium suspension and finally were sub- cultured on selection medium containing Kan 30 and 50 mg/L during the first and second stages after reinforcing cultivation for 20 d. Results of PCR and RT-PCR proved that the aim gene had integrated into alfalfa genome; the transformation rate was 28.4%." @default.
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- W1591567774 date "2009-05-16" @default.
- W1591567774 modified "2023-09-23" @default.
- W1591567774 title "产肠毒素大肠杆菌K88ac-STII-LTA2/LTB融合基因在苜蓿中的表达" @default.
- W1591567774 hasPublicationYear "2009" @default.
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