FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1591619862> ?p ?o ?g. }

• W1591619862 endingPage "51" @default.
• W1591619862 startingPage "34" @default.
• W1591619862 abstract "This paper deals with studying biosynthetic pathways of 2 H-labeled purine ribonucleoside inosine excreted into liquid microbial culture (LC) by Gram-positive chemoheterotrophic bacterium Bacillus subtilis B-3157 while growing of this bacterium on heavy water (HW) medium with 2% (v/v) hydrolysate of deuterated biomass of the methylotrophic bacterium Brevibacterium methylicum B-5662 as a source of 2 H-labeled growth substrates. Isolation of 2 H-labeled inosine from LC was performed by adsorption/desorption on activated carbon with following extraction by 0.3 M ammonium–formate buffer (pH = 8.9), crystallization in 80% (v/v) EtOH, and ion exchange chromatography (IEC) on a column with AG50WX 4 cation exchange resin equilibrated with 0.3 M ammonium–formate buffer and 0.045 M NH 4 Cl. The investigation of deuterium incorporation into the inosine molecule by FAB method demonstrated incorporation of 5 deuterium atoms into the molecule (the total level of deuterium enrichment – 65.5 atom% 2 H) with 3 deuterium atoms being included into the ribose and 2 deuterium atoms – into the hypoxanthine residue of the molecule. Three non-exchangeable deuterium atoms were incorporated into the ribose residue owing to the preservation in this bacterium the minor pathways of de novo glucose biosynthesis in 2 H 2 O-medium. These non-exchangeable deuterium atoms in the ribose residue were originated from HMP shunt reactions, while two other deuterium atoms at C2,C8-positions in the hypoxanthine residue were synthesized from [ 2 H]amino acids, primarily glutamine and glycine, that originated from deuterated hydrolysate. A glycoside proton at b-N 9 -glycosidic bond could be replaced with deuterium via the reaction of ?? 2 elimination at the stage of ribulose-5-monophosphate formation from 3-keto-6-phosphogluconic acid with subsequent proton (deuteron) attachment at the ?1-position of ribulose-5-monophosphate. Two other protons at C2(C3) and C4 positions in ribose residue could be replaced with deuterium via further enzimatic isomerization of ribulose-5-monophosphate into ribose-5-monophosphate. Key words : 2 H-labeled inosine, biosynthesis, biosynthetic pathways, heavy water, Bacillus subtilis" @default.
• W1591619862 created "2016-06-24" @default.
• W1591619862 creator A5002118934 @default.
• W1591619862 creator A5067194907 @default.
• W1591619862 date "2015-01-01" @default.
• W1591619862 modified "2023-09-26" @default.
• W1591619862 title "Bacterium Bacillus Subtilis. Preparation from Bacillus Subtilis B-3157 of 2H-Labeled Purine Ribonucleoside Inosine" @default.
• W1591619862 cites W1508195059 @default.
• W1591619862 cites W1521639687 @default.
• W1591619862 cites W1549231891 @default.
• W1591619862 cites W1865158454 @default.
• W1591619862 cites W187032886 @default.
• W1591619862 cites W1976876223 @default.
• W1591619862 cites W2025533709 @default.
• W1591619862 cites W2032033806 @default.
• W1591619862 cites W2036238228 @default.
• W1591619862 cites W2062247999 @default.
• W1591619862 cites W2070846511 @default.
• W1591619862 cites W2094467764 @default.
• W1591619862 cites W2105648695 @default.
• W1591619862 cites W2123521520 @default.
• W1591619862 cites W2131240251 @default.
• W1591619862 cites W2131834352 @default.
• W1591619862 cites W2167068573 @default.
• W1591619862 cites W2276231149 @default.
• W1591619862 cites W2288364529 @default.
• W1591619862 cites W2323443938 @default.
• W1591619862 cites W2398557674 @default.
• W1591619862 cites W2949990838 @default.
• W1591619862 cites W3214471057 @default.
• W1591619862 cites W652169218 @default.
• W1591619862 hasPublicationYear "2015" @default.
• W1591619862 type Work @default.
• W1591619862 sameAs 1591619862 @default.
• W1591619862 citedByCount "6" @default.
• W1591619862 countsByYear W15916198622015 @default.
• W1591619862 countsByYear W15916198622016 @default.
• W1591619862 crossrefType "journal-article" @default.
• W1591619862 hasAuthorship W1591619862A5002118934 @default.
• W1591619862 hasAuthorship W1591619862A5067194907 @default.
• W1591619862 hasConcept C161790260 @default.
• W1591619862 hasConcept C175605896 @default.
• W1591619862 hasConcept C179998833 @default.
• W1591619862 hasConcept C181199279 @default.
• W1591619862 hasConcept C185592680 @default.
• W1591619862 hasConcept C2775986502 @default.
• W1591619862 hasConcept C2777272437 @default.
• W1591619862 hasConcept C2777610669 @default.
• W1591619862 hasConcept C2778386651 @default.
• W1591619862 hasConcept C2780178953 @default.
• W1591619862 hasConcept C2781116151 @default.
• W1591619862 hasConcept C28800991 @default.
• W1591619862 hasConcept C43617362 @default.
• W1591619862 hasConcept C523546767 @default.
• W1591619862 hasConcept C54355233 @default.
• W1591619862 hasConcept C55493867 @default.
• W1591619862 hasConcept C86803240 @default.
• W1591619862 hasConcept C94412978 @default.
• W1591619862 hasConceptScore W1591619862C161790260 @default.
• W1591619862 hasConceptScore W1591619862C175605896 @default.
• W1591619862 hasConceptScore W1591619862C179998833 @default.
• W1591619862 hasConceptScore W1591619862C181199279 @default.
• W1591619862 hasConceptScore W1591619862C185592680 @default.
• W1591619862 hasConceptScore W1591619862C2775986502 @default.
• W1591619862 hasConceptScore W1591619862C2777272437 @default.
• W1591619862 hasConceptScore W1591619862C2777610669 @default.
• W1591619862 hasConceptScore W1591619862C2778386651 @default.
• W1591619862 hasConceptScore W1591619862C2780178953 @default.
• W1591619862 hasConceptScore W1591619862C2781116151 @default.
• W1591619862 hasConceptScore W1591619862C28800991 @default.
• W1591619862 hasConceptScore W1591619862C43617362 @default.
• W1591619862 hasConceptScore W1591619862C523546767 @default.
• W1591619862 hasConceptScore W1591619862C54355233 @default.
• W1591619862 hasConceptScore W1591619862C55493867 @default.
• W1591619862 hasConceptScore W1591619862C86803240 @default.
• W1591619862 hasConceptScore W1591619862C94412978 @default.
• W1591619862 hasLocation W15916198621 @default.
• W1591619862 hasOpenAccess W1591619862 @default.
• W1591619862 hasPrimaryLocation W15916198621 @default.
• W1591619862 hasRelatedWork W1486288254 @default.
• W1591619862 hasRelatedWork W1493397898 @default.
• W1591619862 hasRelatedWork W1497944282 @default.
• W1591619862 hasRelatedWork W1501635534 @default.
• W1591619862 hasRelatedWork W1510902715 @default.
• W1591619862 hasRelatedWork W1521639687 @default.
• W1591619862 hasRelatedWork W1525884025 @default.
• W1591619862 hasRelatedWork W1549231891 @default.
• W1591619862 hasRelatedWork W1552114712 @default.
• W1591619862 hasRelatedWork W1560608802 @default.
• W1591619862 hasRelatedWork W1561542747 @default.
• W1591619862 hasRelatedWork W1603748312 @default.
• W1591619862 hasRelatedWork W1610501143 @default.
• W1591619862 hasRelatedWork W1676659178 @default.
• W1591619862 hasRelatedWork W1756232742 @default.
• W1591619862 hasRelatedWork W1920260933 @default.
• W1591619862 hasRelatedWork W2110298489 @default.
• W1591619862 hasRelatedWork W2339984090 @default.
• W1591619862 hasRelatedWork W2401361487 @default.

• next