Matches in SemOpenAlex for { <https://semopenalex.org/work/W1591661445> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W1591661445 endingPage "1323" @default.
- W1591661445 startingPage "1318" @default.
- W1591661445 abstract "Abstract Thymidylate synthetase from Don Chinese hamster cells grown in vitro was characterized and the changes in the specific activity of this enzyme during the culture cycle and the cell cycle were examined. In crude extracts thymidylate synthetase exhibited a Km for dUMP of 0.90 ± 0.04 x 10-5 m, was very heat-labile at temperatures above 43°, but was stable over pH 7.5 to 9.5. During the culture cycle of Don cells, thymidylate synthetase activity rose 6- to 8-fold during logarithmic growth and fell to base line when the cultures entered stationary phase. In contrast, lactate dehydrogenase activity was low during logarithmic growth and high during stationary phase. However, during the cell cycle of logarithmically growing Don C cells (Don subline), thymidylate synthetase and lactate dehydrogenase activities rose and fell in exact parallel, being low in G1, rising sharply at the beginning of S and again in late S, dropping at the beginning of G2, and then rising again in mid-G2. These studies indicate that, while the absolute amount of a given enzyme present at any time in a cell may be controlled individually for different enzymes, regulation of the relative activity and perhaps the general synthesis and degradation of many different enzymes is synchronized within the cell during logarithmic growth." @default.
- W1591661445 created "2016-06-24" @default.
- W1591661445 creator A5030227842 @default.
- W1591661445 date "1971-03-01" @default.
- W1591661445 modified "2023-10-11" @default.
- W1591661445 title "Thymidylate Synthetase Activity in Cultured Mammalian Cells" @default.
- W1591661445 cites W108364404 @default.
- W1591661445 cites W109577459 @default.
- W1591661445 cites W1486522428 @default.
- W1591661445 cites W1530336911 @default.
- W1591661445 cites W1544308022 @default.
- W1591661445 cites W1565890402 @default.
- W1591661445 cites W1600179333 @default.
- W1591661445 cites W16336426 @default.
- W1591661445 cites W164824161 @default.
- W1591661445 cites W169794223 @default.
- W1591661445 cites W1775749144 @default.
- W1591661445 cites W1921289296 @default.
- W1591661445 cites W1978239826 @default.
- W1591661445 cites W1978461711 @default.
- W1591661445 cites W1981138087 @default.
- W1591661445 cites W1990988502 @default.
- W1591661445 cites W2008949082 @default.
- W1591661445 cites W2023986404 @default.
- W1591661445 cites W2033713783 @default.
- W1591661445 cites W2034505800 @default.
- W1591661445 cites W2035549311 @default.
- W1591661445 cites W2036544276 @default.
- W1591661445 cites W2041295526 @default.
- W1591661445 cites W2043044080 @default.
- W1591661445 cites W2051760741 @default.
- W1591661445 cites W2053387982 @default.
- W1591661445 cites W2055525958 @default.
- W1591661445 cites W2056782631 @default.
- W1591661445 cites W2073749796 @default.
- W1591661445 cites W2073938212 @default.
- W1591661445 cites W2082782677 @default.
- W1591661445 cites W2093158828 @default.
- W1591661445 cites W2115267974 @default.
- W1591661445 cites W2129413541 @default.
- W1591661445 cites W2144480104 @default.
- W1591661445 cites W2168597255 @default.
- W1591661445 cites W2323604581 @default.
- W1591661445 cites W23529498 @default.
- W1591661445 cites W2395739546 @default.
- W1591661445 cites W2401814200 @default.
- W1591661445 cites W32025885 @default.
- W1591661445 cites W53300029 @default.
- W1591661445 doi "https://doi.org/10.1016/s0021-9258(19)76977-4" @default.
- W1591661445 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5545076" @default.
- W1591661445 hasPublicationYear "1971" @default.
- W1591661445 type Work @default.
- W1591661445 sameAs 1591661445 @default.
- W1591661445 citedByCount "53" @default.
- W1591661445 crossrefType "journal-article" @default.
- W1591661445 hasAuthorship W1591661445A5030227842 @default.
- W1591661445 hasBestOaLocation W15916614451 @default.
- W1591661445 hasConcept C121608353 @default.
- W1591661445 hasConcept C123321153 @default.
- W1591661445 hasConcept C153911025 @default.
- W1591661445 hasConcept C185592680 @default.
- W1591661445 hasConcept C2780456651 @default.
- W1591661445 hasConcept C54355233 @default.
- W1591661445 hasConcept C55493867 @default.
- W1591661445 hasConcept C86803240 @default.
- W1591661445 hasConcept C95444343 @default.
- W1591661445 hasConceptScore W1591661445C121608353 @default.
- W1591661445 hasConceptScore W1591661445C123321153 @default.
- W1591661445 hasConceptScore W1591661445C153911025 @default.
- W1591661445 hasConceptScore W1591661445C185592680 @default.
- W1591661445 hasConceptScore W1591661445C2780456651 @default.
- W1591661445 hasConceptScore W1591661445C54355233 @default.
- W1591661445 hasConceptScore W1591661445C55493867 @default.
- W1591661445 hasConceptScore W1591661445C86803240 @default.
- W1591661445 hasConceptScore W1591661445C95444343 @default.
- W1591661445 hasIssue "5" @default.
- W1591661445 hasLocation W15916614451 @default.
- W1591661445 hasOpenAccess W1591661445 @default.
- W1591661445 hasPrimaryLocation W15916614451 @default.
- W1591661445 hasRelatedWork W1489098022 @default.
- W1591661445 hasRelatedWork W1984994946 @default.
- W1591661445 hasRelatedWork W1993875123 @default.
- W1591661445 hasRelatedWork W2010366112 @default.
- W1591661445 hasRelatedWork W2082860237 @default.
- W1591661445 hasRelatedWork W2137989529 @default.
- W1591661445 hasRelatedWork W2153582959 @default.
- W1591661445 hasRelatedWork W2392930094 @default.
- W1591661445 hasRelatedWork W3029889643 @default.
- W1591661445 hasRelatedWork W3031205269 @default.
- W1591661445 hasVolume "246" @default.
- W1591661445 isParatext "false" @default.
- W1591661445 isRetracted "false" @default.
- W1591661445 magId "1591661445" @default.
- W1591661445 workType "article" @default.