Matches in SemOpenAlex for { <https://semopenalex.org/work/W1591775923> ?p ?o ?g. }
- W1591775923 abstract "The adsorption of proteins and synthetic polymers at the air-water interface (AWI) has broad significance in biomedicine and biotechnology. Protein behavior at the AWI can be guided to control the structure of the two-dimensional biopolymer film. In addition, synthetic polymers affect how plasma proteins act on implant or drug carrier surfaces, and can also decrease the potential for gas embolism. In this thesis, fluorescence microscopy was applied in combination with tensiometry and atomic force microscopy to study plasma proteins at the AWI alone and under the effect of synthetic polymers. First, the morphology of serum albumin layer controlled by the solution conditions was explored by fluorescence microscopy. Heterogeneity at the micron scale was observed for the protein film during adsorption and at reduced concentrations. Moreover, the competition for interfacial area between Pluronic surfactant F-127 and fibrinogen or serum albumin was studied by semi-quantitative confocal fluorescence methods. A transition stage where F-127 and protein underwent lateral phase separation was found. Two competing processes were revealed – the disintegration of protein-rich phase by F-127 and the coalescence of protein phase. Lastly, the interaction of immunoglobulin and the thin film of a widely used polydimethylsiloxane synthetic polymer was studied at the AWI. The compression state of the polymer film was shown to significantly affect protein adsorption and guide proteins into circular domain structures. Overall, this work has demonstrated the wide utility of fluorescence microscopy to study protein-protein and protein-synthetic polymer interactions at the AWI." @default.
- W1591775923 created "2016-06-24" @default.
- W1591775923 creator A5050275030 @default.
- W1591775923 date "2013-01-01" @default.
- W1591775923 modified "2023-09-27" @default.
- W1591775923 title "Plasma Proteins and Their interaction With Synthetic Polymers at the Air-Water interface" @default.
- W1591775923 cites W1486982813 @default.
- W1591775923 cites W1523381510 @default.
- W1591775923 cites W1529608215 @default.
- W1591775923 cites W1535562724 @default.
- W1591775923 cites W1559244578 @default.
- W1591775923 cites W1585161971 @default.
- W1591775923 cites W1588000118 @default.
- W1591775923 cites W1599568592 @default.
- W1591775923 cites W1775749144 @default.
- W1591775923 cites W1849677834 @default.
- W1591775923 cites W1963925934 @default.
- W1591775923 cites W1964000824 @default.
- W1591775923 cites W1965442427 @default.
- W1591775923 cites W1969604041 @default.
- W1591775923 cites W1969733268 @default.
- W1591775923 cites W1969893670 @default.
- W1591775923 cites W1970863842 @default.
- W1591775923 cites W1971674915 @default.
- W1591775923 cites W1971921706 @default.
- W1591775923 cites W1976334100 @default.
- W1591775923 cites W1976902210 @default.
- W1591775923 cites W1980479545 @default.
- W1591775923 cites W1980599353 @default.
- W1591775923 cites W1980769354 @default.
- W1591775923 cites W1984636337 @default.
- W1591775923 cites W1986523268 @default.
- W1591775923 cites W1987594127 @default.
- W1591775923 cites W1989512004 @default.
- W1591775923 cites W1990969916 @default.
- W1591775923 cites W1991322453 @default.
- W1591775923 cites W1993017089 @default.
- W1591775923 cites W1993411624 @default.
- W1591775923 cites W1994537827 @default.
- W1591775923 cites W1995995697 @default.
- W1591775923 cites W1996654743 @default.
- W1591775923 cites W1999163577 @default.
- W1591775923 cites W2000693577 @default.
- W1591775923 cites W2002052849 @default.
- W1591775923 cites W2003689388 @default.
- W1591775923 cites W2003904008 @default.
- W1591775923 cites W2004313685 @default.
- W1591775923 cites W2005640380 @default.
- W1591775923 cites W2006225923 @default.
- W1591775923 cites W2006322736 @default.
- W1591775923 cites W2007272217 @default.
- W1591775923 cites W2009339317 @default.
- W1591775923 cites W2009619344 @default.
- W1591775923 cites W2010017832 @default.
- W1591775923 cites W2010018293 @default.
- W1591775923 cites W2010602998 @default.
- W1591775923 cites W2010711853 @default.
- W1591775923 cites W2010920019 @default.
- W1591775923 cites W2011657990 @default.
- W1591775923 cites W2014861094 @default.
- W1591775923 cites W2015517515 @default.
- W1591775923 cites W2018632301 @default.
- W1591775923 cites W2022315563 @default.
- W1591775923 cites W2024745799 @default.
- W1591775923 cites W2025457908 @default.
- W1591775923 cites W2025826365 @default.
- W1591775923 cites W2025859600 @default.
- W1591775923 cites W2025938842 @default.
- W1591775923 cites W2026093166 @default.
- W1591775923 cites W2026719741 @default.
- W1591775923 cites W2026896696 @default.
- W1591775923 cites W2027397388 @default.
- W1591775923 cites W2029136818 @default.
- W1591775923 cites W2030276712 @default.
- W1591775923 cites W2031096293 @default.
- W1591775923 cites W2031367101 @default.
- W1591775923 cites W2039580510 @default.
- W1591775923 cites W2039870629 @default.
- W1591775923 cites W2041856284 @default.
- W1591775923 cites W2042846045 @default.
- W1591775923 cites W2043367471 @default.
- W1591775923 cites W2043412894 @default.
- W1591775923 cites W2043623277 @default.
- W1591775923 cites W2043699353 @default.
- W1591775923 cites W2044252957 @default.
- W1591775923 cites W2047125616 @default.
- W1591775923 cites W2047205370 @default.
- W1591775923 cites W2047391094 @default.
- W1591775923 cites W2047406153 @default.
- W1591775923 cites W2048814035 @default.
- W1591775923 cites W2049343111 @default.
- W1591775923 cites W2050871125 @default.
- W1591775923 cites W2051494388 @default.
- W1591775923 cites W2051600836 @default.
- W1591775923 cites W2052627492 @default.
- W1591775923 cites W2052629987 @default.
- W1591775923 cites W2053436725 @default.
- W1591775923 cites W2056558361 @default.
- W1591775923 cites W2057544418 @default.
- W1591775923 cites W2059327711 @default.