Matches in SemOpenAlex for { <https://semopenalex.org/work/W1592024261> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W1592024261 endingPage "13851" @default.
- W1592024261 startingPage "13849" @default.
- W1592024261 abstract "Several synthetic DNAs were prepared containing the unusual bases 7-deazaadenine (c7A) and 7-deazaguanine (c7G). As judged from changes in melting temperatures these modified DNAs bound ethidium to a similar extent as the parent polymers. However, duplexes such as poly [d(Tc7G)].poly[d(CA)] and poly[d-(TC)].poly[d(c7GA]) gave no enhancement of ethidium fluorescence in a standard ethidium fluorescence assay. Fluorescence spectra in the range 400-650 nm showed that ethidium bound to poly[d(TC)].poly[d(Gc7A)] gave 70% of the fluorescence of the parent polymer poly[d(TC)].poly[d(GA)], whereas the fluorescence of poly[d(TC)].poly[d(c7GA)] was essentially 0%. Even the intrinsic fluorescence of ethidium in solution was quenched in the presence of poly[d(TC)].poly[d(c7GA)]. Binding constants were estimated from Scatchard analysis and were 4.8, 3.4, and 2.0 x 10(6) M-1 for poly[d(TC)].poly[d(GA)], poly[d(TC)].poly[d(Gc7A)], and poly[d(TC)].poly[d(c7GA)], respectively. This reduction in binding constant cannot account for the loss of fluorescence. The UV spectrum of ethidium was measured in the presence of these DNAs, and some significant differences were noted. Presumably the presence of 7-deazaguanine alters the electronic structure of bound ethidium so that it can no longer fluoresce." @default.
- W1592024261 created "2016-06-24" @default.
- W1592024261 creator A5013431958 @default.
- W1592024261 creator A5035436829 @default.
- W1592024261 date "1991-07-01" @default.
- W1592024261 modified "2023-10-16" @default.
- W1592024261 title "Ethidium bromide does not fluoresce when intercalated adjacent to 7-deazaguanine in duplex DNA" @default.
- W1592024261 doi "https://doi.org/10.1016/s0021-9258(18)92779-1" @default.
- W1592024261 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1856218" @default.
- W1592024261 hasPublicationYear "1991" @default.
- W1592024261 type Work @default.
- W1592024261 sameAs 1592024261 @default.
- W1592024261 citedByCount "49" @default.
- W1592024261 countsByYear W15920242612012 @default.
- W1592024261 countsByYear W15920242612013 @default.
- W1592024261 countsByYear W15920242612014 @default.
- W1592024261 countsByYear W15920242612015 @default.
- W1592024261 countsByYear W15920242612016 @default.
- W1592024261 countsByYear W15920242612017 @default.
- W1592024261 countsByYear W15920242612018 @default.
- W1592024261 countsByYear W15920242612021 @default.
- W1592024261 countsByYear W15920242612022 @default.
- W1592024261 crossrefType "journal-article" @default.
- W1592024261 hasAuthorship W1592024261A5013431958 @default.
- W1592024261 hasAuthorship W1592024261A5035436829 @default.
- W1592024261 hasBestOaLocation W15920242611 @default.
- W1592024261 hasConcept C120665830 @default.
- W1592024261 hasConcept C121332964 @default.
- W1592024261 hasConcept C12554922 @default.
- W1592024261 hasConcept C153911025 @default.
- W1592024261 hasConcept C179104552 @default.
- W1592024261 hasConcept C185592680 @default.
- W1592024261 hasConcept C2776300020 @default.
- W1592024261 hasConcept C2776907736 @default.
- W1592024261 hasConcept C552990157 @default.
- W1592024261 hasConcept C55493867 @default.
- W1592024261 hasConcept C86803240 @default.
- W1592024261 hasConcept C91881484 @default.
- W1592024261 hasConcept C99611785 @default.
- W1592024261 hasConceptScore W1592024261C120665830 @default.
- W1592024261 hasConceptScore W1592024261C121332964 @default.
- W1592024261 hasConceptScore W1592024261C12554922 @default.
- W1592024261 hasConceptScore W1592024261C153911025 @default.
- W1592024261 hasConceptScore W1592024261C179104552 @default.
- W1592024261 hasConceptScore W1592024261C185592680 @default.
- W1592024261 hasConceptScore W1592024261C2776300020 @default.
- W1592024261 hasConceptScore W1592024261C2776907736 @default.
- W1592024261 hasConceptScore W1592024261C552990157 @default.
- W1592024261 hasConceptScore W1592024261C55493867 @default.
- W1592024261 hasConceptScore W1592024261C86803240 @default.
- W1592024261 hasConceptScore W1592024261C91881484 @default.
- W1592024261 hasConceptScore W1592024261C99611785 @default.
- W1592024261 hasIssue "21" @default.
- W1592024261 hasLocation W15920242611 @default.
- W1592024261 hasOpenAccess W1592024261 @default.
- W1592024261 hasPrimaryLocation W15920242611 @default.
- W1592024261 hasRelatedWork W1987586745 @default.
- W1592024261 hasRelatedWork W2027093891 @default.
- W1592024261 hasRelatedWork W2031898952 @default.
- W1592024261 hasRelatedWork W2036247818 @default.
- W1592024261 hasRelatedWork W2037236039 @default.
- W1592024261 hasRelatedWork W2066600330 @default.
- W1592024261 hasRelatedWork W2069750659 @default.
- W1592024261 hasRelatedWork W2145381472 @default.
- W1592024261 hasRelatedWork W2273575022 @default.
- W1592024261 hasRelatedWork W4213116877 @default.
- W1592024261 hasVolume "266" @default.
- W1592024261 isParatext "false" @default.
- W1592024261 isRetracted "false" @default.
- W1592024261 magId "1592024261" @default.
- W1592024261 workType "article" @default.