Matches in SemOpenAlex for { <https://semopenalex.org/work/W1593005511> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W1593005511 endingPage "4" @default.
- W1593005511 startingPage "290" @default.
- W1593005511 abstract "DAB389 GRP is composed of the catalytic and transmembrane domains of diphtheria toxin fused to gastrin-releasing peptide (GRP). DAB389 GRP is selectively targeted to, and inhibits protein synthesis in, cell lines expressing GRP receptors. Protein synthesis in 5'ET4 cells (BALB/3T3 fibroblasts transfected with the gene encoding the GRP receptor) was inhibited by 50% in the presence of 20 pM DAB389 GRP (IC50, 20 pM). DAB389 GRP did not inhibit protein synthesis in untransfected BALB/3T3 cells. A second neuropeptide-conjugated toxin, DAB389 SP, directed to cells expressing substance P receptors, was not cytotoxic to 5'ET4 cells, nor was DAB389 GRP cytotoxic to substance P receptor-bearing cells. DAB389 GRP cytotoxic effects were receptor specific and were inhibited either by excess GRP or anti-GRP antibody. Cytotoxicity was mediated by passage through an acidic vesicle, because addition of 10 microM chloroquine to the reaction inhibited cytotoxicity. DAB389 GRP and DAB389 SP were tested on a number of tumor cell lines. DAB389 GRP inhibited protein synthesis in AR42J rat pancreatic acinar cells and HuTu 80 human duodenal adenocarcinoma cells with IC50s of 65 and 200 pM, respectively. DAB389 SP had an IC50 of 9.5 pM for the AR42J cells and 12 nM for the HuTu 80 cell line. A number of small cell lung cancer cell (SCLC) lines were tested, and the IC50 for DAB389 GRP ranged from 1.1 to 85 nM. Sensitivity to DAB389 GRP appeared to be based on receptor number and receptor type (i.e., GRP or neuromedin B preferring). SCLC cells were also sensitive to DAB389 SP, with IC50s ranging from 2.4 to 11.5 nM. These results suggest that a potential use exists for diphtheria-based fusion toxins as therapeutic agents for treatment of SCLC and other neuropeptide receptor-bearing cancers." @default.
- W1593005511 created "2016-06-24" @default.
- W1593005511 creator A5005161415 @default.
- W1593005511 creator A5022123296 @default.
- W1593005511 creator A5036639335 @default.
- W1593005511 creator A5041036479 @default.
- W1593005511 creator A5047102000 @default.
- W1593005511 creator A5057729043 @default.
- W1593005511 date "1997-01-15" @default.
- W1593005511 modified "2023-09-23" @default.
- W1593005511 title "Inhibition of protein synthesis in small cell lung cancer cells induced by the diphtheria toxin-related fusion protein DAB389 GRP." @default.
- W1593005511 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9000570" @default.
- W1593005511 hasPublicationYear "1997" @default.
- W1593005511 type Work @default.
- W1593005511 sameAs 1593005511 @default.
- W1593005511 citedByCount "6" @default.
- W1593005511 countsByYear W15930055112015 @default.
- W1593005511 countsByYear W15930055112016 @default.
- W1593005511 countsByYear W15930055112019 @default.
- W1593005511 countsByYear W15930055112021 @default.
- W1593005511 crossrefType "journal-article" @default.
- W1593005511 hasAuthorship W1593005511A5005161415 @default.
- W1593005511 hasAuthorship W1593005511A5022123296 @default.
- W1593005511 hasAuthorship W1593005511A5036639335 @default.
- W1593005511 hasAuthorship W1593005511A5041036479 @default.
- W1593005511 hasAuthorship W1593005511A5047102000 @default.
- W1593005511 hasAuthorship W1593005511A5057729043 @default.
- W1593005511 hasConcept C109316439 @default.
- W1593005511 hasConcept C118303440 @default.
- W1593005511 hasConcept C153911025 @default.
- W1593005511 hasConcept C154317977 @default.
- W1593005511 hasConcept C170493617 @default.
- W1593005511 hasConcept C185592680 @default.
- W1593005511 hasConcept C202751555 @default.
- W1593005511 hasConcept C2776998989 @default.
- W1593005511 hasConcept C2777367657 @default.
- W1593005511 hasConcept C2778448772 @default.
- W1593005511 hasConcept C33057221 @default.
- W1593005511 hasConcept C54009773 @default.
- W1593005511 hasConcept C54355233 @default.
- W1593005511 hasConcept C55493867 @default.
- W1593005511 hasConcept C81885089 @default.
- W1593005511 hasConcept C86803240 @default.
- W1593005511 hasConceptScore W1593005511C109316439 @default.
- W1593005511 hasConceptScore W1593005511C118303440 @default.
- W1593005511 hasConceptScore W1593005511C153911025 @default.
- W1593005511 hasConceptScore W1593005511C154317977 @default.
- W1593005511 hasConceptScore W1593005511C170493617 @default.
- W1593005511 hasConceptScore W1593005511C185592680 @default.
- W1593005511 hasConceptScore W1593005511C202751555 @default.
- W1593005511 hasConceptScore W1593005511C2776998989 @default.
- W1593005511 hasConceptScore W1593005511C2777367657 @default.
- W1593005511 hasConceptScore W1593005511C2778448772 @default.
- W1593005511 hasConceptScore W1593005511C33057221 @default.
- W1593005511 hasConceptScore W1593005511C54009773 @default.
- W1593005511 hasConceptScore W1593005511C54355233 @default.
- W1593005511 hasConceptScore W1593005511C55493867 @default.
- W1593005511 hasConceptScore W1593005511C81885089 @default.
- W1593005511 hasConceptScore W1593005511C86803240 @default.
- W1593005511 hasIssue "2" @default.
- W1593005511 hasLocation W15930055111 @default.
- W1593005511 hasOpenAccess W1593005511 @default.
- W1593005511 hasPrimaryLocation W15930055111 @default.
- W1593005511 hasRelatedWork W1610525484 @default.
- W1593005511 hasRelatedWork W1997153920 @default.
- W1593005511 hasRelatedWork W2003123504 @default.
- W1593005511 hasRelatedWork W2005523708 @default.
- W1593005511 hasRelatedWork W2058463700 @default.
- W1593005511 hasRelatedWork W2068965074 @default.
- W1593005511 hasRelatedWork W2347120132 @default.
- W1593005511 hasRelatedWork W2352704199 @default.
- W1593005511 hasRelatedWork W2354948779 @default.
- W1593005511 hasRelatedWork W4206956544 @default.
- W1593005511 hasVolume "57" @default.
- W1593005511 isParatext "false" @default.
- W1593005511 isRetracted "false" @default.
- W1593005511 magId "1593005511" @default.
- W1593005511 workType "article" @default.