Matches in SemOpenAlex for { <https://semopenalex.org/work/W1593145318> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W1593145318 endingPage "18030" @default.
- W1593145318 startingPage "18024" @default.
- W1593145318 abstract "2-Cyano-4-hydroxycinnamate was covalently linked, through a diazo bond, to Sepharose 4B, which had been elongated with a hydrophobic spacer. A Triton X-100 extract from bovine heart mitochondria was pre-purified by hydroxylapatite chromatography and passed through the 2-cyano-4-hydroxycinnamate affinity resin in the presence of 0.7% deoxycholate. At pH 6 and in the presence of 0.2 M sodium chloride, a single polypeptide with an Mr of 34,000 was eluted. Subsequently, at pH 8 and in the presence of 2-cyano-4-hydroxycinnamate, another single protein with an Mr of 31,500 was released. Both proteins were reconstituted into phospholipid vesicles and their transport activities were measured. High, delta pH-dependent, 2-cyanocinnamate-sensitive pyruvate uptake was measured in vesicles containing only the 34-kDa protein. alpha-Ketobutyrate and other alpha-ketomonocarboxylic acids were competitive inhibitors of the pyruvate uptake, whereas di- and tricarboxylates had only small effects. alpha-Ketoglutarate-alpha-ketoglutarate exchange could only be measured in vesicles containing the 31.5-kDa protein. The molecular weight of this protein and its functional properties were similar to those of the alpha-ketoglutarate carrier isolated by a different method (Bisaccia, Indiveri, C., and Palmieri, F. (1985) Biochim. Biophys. Acta 810, 362-369). 2-Cyano-4-hydroxycinnamate inhibited the alpha-ketoglutarate exchange in a noncompetitive manner with an apparent Ki of 0.7 mM. It is concluded that by the described affinity chromatography procedure, two mitochondrial carriers transporting alpha-ketoacids, i.e. the monocarboxylate and the alpha-ketoglutarate carrier, could be purified in a functionally active state." @default.
- W1593145318 created "2016-06-24" @default.
- W1593145318 creator A5034923377 @default.
- W1593145318 creator A5042840198 @default.
- W1593145318 creator A5052099970 @default.
- W1593145318 date "1989-10-01" @default.
- W1593145318 modified "2023-10-17" @default.
- W1593145318 title "Monocarboxylate and α-ketoglutarate carriers from bovine heart mitochondria" @default.
- W1593145318 cites W1484115349 @default.
- W1593145318 cites W1550650979 @default.
- W1593145318 cites W1562313724 @default.
- W1593145318 cites W1566169309 @default.
- W1593145318 cites W1577519912 @default.
- W1593145318 cites W1605182467 @default.
- W1593145318 cites W1775749144 @default.
- W1593145318 cites W1845254999 @default.
- W1593145318 cites W1857517682 @default.
- W1593145318 cites W1963599172 @default.
- W1593145318 cites W1967188028 @default.
- W1593145318 cites W1970013901 @default.
- W1593145318 cites W1976739702 @default.
- W1593145318 cites W1978678285 @default.
- W1593145318 cites W1992484634 @default.
- W1593145318 cites W1993246331 @default.
- W1593145318 cites W2008840452 @default.
- W1593145318 cites W2014185015 @default.
- W1593145318 cites W2021467990 @default.
- W1593145318 cites W2026746069 @default.
- W1593145318 cites W2027543395 @default.
- W1593145318 cites W2029082121 @default.
- W1593145318 cites W2031182339 @default.
- W1593145318 cites W2031893089 @default.
- W1593145318 cites W2041763710 @default.
- W1593145318 cites W2051857619 @default.
- W1593145318 cites W2070739780 @default.
- W1593145318 cites W2075564316 @default.
- W1593145318 cites W2081581585 @default.
- W1593145318 cites W2086312953 @default.
- W1593145318 cites W2087922339 @default.
- W1593145318 cites W2088869962 @default.
- W1593145318 cites W2088972842 @default.
- W1593145318 cites W2100837269 @default.
- W1593145318 cites W2115708290 @default.
- W1593145318 cites W2130692096 @default.
- W1593145318 cites W2150630343 @default.
- W1593145318 cites W2153334627 @default.
- W1593145318 cites W2241117563 @default.
- W1593145318 doi "https://doi.org/10.1016/s0021-9258(19)84674-4" @default.
- W1593145318 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2808362" @default.
- W1593145318 hasPublicationYear "1989" @default.
- W1593145318 type Work @default.
- W1593145318 sameAs 1593145318 @default.
- W1593145318 citedByCount "44" @default.
- W1593145318 countsByYear W15931453182012 @default.
- W1593145318 countsByYear W15931453182013 @default.
- W1593145318 countsByYear W15931453182015 @default.
- W1593145318 countsByYear W15931453182016 @default.
- W1593145318 countsByYear W15931453182017 @default.
- W1593145318 countsByYear W15931453182019 @default.
- W1593145318 crossrefType "journal-article" @default.
- W1593145318 hasAuthorship W1593145318A5034923377 @default.
- W1593145318 hasAuthorship W1593145318A5042840198 @default.
- W1593145318 hasAuthorship W1593145318A5052099970 @default.
- W1593145318 hasBestOaLocation W15931453181 @default.
- W1593145318 hasConcept C185592680 @default.
- W1593145318 hasConcept C28859421 @default.
- W1593145318 hasConcept C55493867 @default.
- W1593145318 hasConceptScore W1593145318C185592680 @default.
- W1593145318 hasConceptScore W1593145318C28859421 @default.
- W1593145318 hasConceptScore W1593145318C55493867 @default.
- W1593145318 hasIssue "30" @default.
- W1593145318 hasLocation W15931453181 @default.
- W1593145318 hasOpenAccess W1593145318 @default.
- W1593145318 hasPrimaryLocation W15931453181 @default.
- W1593145318 hasRelatedWork W122755718 @default.
- W1593145318 hasRelatedWork W1523953204 @default.
- W1593145318 hasRelatedWork W2016356419 @default.
- W1593145318 hasRelatedWork W2023285574 @default.
- W1593145318 hasRelatedWork W2045818454 @default.
- W1593145318 hasRelatedWork W2053403842 @default.
- W1593145318 hasRelatedWork W2073919841 @default.
- W1593145318 hasRelatedWork W2074223515 @default.
- W1593145318 hasRelatedWork W2394954531 @default.
- W1593145318 hasRelatedWork W1474365976 @default.
- W1593145318 hasVolume "264" @default.
- W1593145318 isParatext "false" @default.
- W1593145318 isRetracted "false" @default.
- W1593145318 magId "1593145318" @default.
- W1593145318 workType "article" @default.