FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1593540164> ?p ?o ?g. }

• W1593540164 endingPage "11720" @default.
• W1593540164 startingPage "11714" @default.
• W1593540164 abstract "The kinase and sugar phosphate exchange reactions of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase were inactivated by treatment with 5'-p-fluorosulfonylbenzoyladenosine or 8-azido-ATP, but activity could be restored by the addition of dithiothreitol. This inactivation was accompanied by incorporation of 5'-p-sulfonylbenzoyl[8-14C]adenosine into the enzyme that was not released by the addition of dithiothreitol. The lack of effect of ATP analogs on the ATP/ADP exchange or on bisphosphatase activity and reversal of their effects on the kinase and sugar phosphate reactions by dithiothreitol suggest that 1) they reacted with sulfhydryl groups important for sugar phosphate binding in the kinase reaction, and 2) the inactivation of the kinase by these analogs involves a specific reaction that is not related to their general mechanism of attacking nucleotide-binding sites. In addition, alkylation of the enzymes' sulfhydryls with iodoacetamide prevented inactivation by 5'-p-fluorosulfonylbenzoyladenosine, suggesting that the same thiols were involved. o-Iodosobenzoate inactivated the kinase and sugar phosphate exchange; the inactivation was reversed by dithiothreitol; but there was no effect on the bisphosphatase or nucleotide exchange, indicating that oxidation occurred at the same sulfhydryl that are associated with sugar phosphate binding. ATP or ADP, but not fructose 6-phosphate, protected these groups from modification by 5'-p-fluorosulfonylbenzoyladenosine, 8-azido-ATP, and o-iodosobenzoate. ATP also induced dramatic changes in the circular dichroism spectrum of the enzyme, suggesting that adenine nucleotide protection of thiol groups resulted from changes in enzyme secondary structure. Analysis of cyanogen bromide fragments of 14C-carboxamidomethylated enzyme showed that all radioactivity was associated with cysteinyl residues in a single cyanogen bromide fragment. Three of these cysteinyl residues are clustered in a 38-residue region, which probably plays a role in maintaining the conformation of the kinase sugar phosphate-binding site." @default.
• W1593540164 created "2016-06-24" @default.
• W1593540164 creator A5025785938 @default.
• W1593540164 creator A5038982579 @default.
• W1593540164 creator A5041495264 @default.
• W1593540164 creator A5044516788 @default.
• W1593540164 creator A5048018962 @default.
• W1593540164 creator A5078482941 @default.
• W1593540164 date "1987-08-01" @default.
• W1593540164 modified "2023-10-14" @default.
• W1593540164 title "Rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. Identification of essential sulfhydryl residues in the primary sequence of the enzyme." @default.
• W1593540164 cites W1481006558 @default.
• W1593540164 cites W1508384599 @default.
• W1593540164 cites W1516648662 @default.
• W1593540164 cites W1516876967 @default.
• W1593540164 cites W1522933116 @default.
• W1593540164 cites W1526655187 @default.
• W1593540164 cites W1533115557 @default.
• W1593540164 cites W1540674180 @default.
• W1593540164 cites W1558998530 @default.
• W1593540164 cites W1568680367 @default.
• W1593540164 cites W1591608688 @default.
• W1593540164 cites W1595095757 @default.
• W1593540164 cites W2014550271 @default.
• W1593540164 cites W2017373178 @default.
• W1593540164 cites W2036474910 @default.
• W1593540164 cites W2149568310 @default.
• W1593540164 cites W2180056482 @default.
• W1593540164 cites W2251920431 @default.
• W1593540164 doi "https://doi.org/10.1016/s0021-9258(18)60869-5" @default.
• W1593540164 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3040713" @default.
• W1593540164 hasPublicationYear "1987" @default.
• W1593540164 type Work @default.
• W1593540164 sameAs 1593540164 @default.
• W1593540164 citedByCount "20" @default.
• W1593540164 countsByYear W15935401642012 @default.
• W1593540164 crossrefType "journal-article" @default.
• W1593540164 hasAuthorship W1593540164A5025785938 @default.
• W1593540164 hasAuthorship W1593540164A5038982579 @default.
• W1593540164 hasAuthorship W1593540164A5041495264 @default.
• W1593540164 hasAuthorship W1593540164A5044516788 @default.
• W1593540164 hasAuthorship W1593540164A5048018962 @default.
• W1593540164 hasAuthorship W1593540164A5078482941 @default.
• W1593540164 hasBestOaLocation W15935401641 @default.
• W1593540164 hasConcept C104317684 @default.
• W1593540164 hasConcept C116834253 @default.
• W1593540164 hasConcept C121332964 @default.
• W1593540164 hasConcept C1276947 @default.
• W1593540164 hasConcept C167625842 @default.
• W1593540164 hasConcept C181199279 @default.
• W1593540164 hasConcept C185592680 @default.
• W1593540164 hasConcept C189909181 @default.
• W1593540164 hasConcept C20251656 @default.
• W1593540164 hasConcept C2776970464 @default.
• W1593540164 hasConcept C2777977315 @default.
• W1593540164 hasConcept C2778112365 @default.
• W1593540164 hasConcept C2780854706 @default.
• W1593540164 hasConcept C55493867 @default.
• W1593540164 hasConcept C58431802 @default.
• W1593540164 hasConcept C59822182 @default.
• W1593540164 hasConcept C86803240 @default.
• W1593540164 hasConcept C92825506 @default.
• W1593540164 hasConceptScore W1593540164C104317684 @default.
• W1593540164 hasConceptScore W1593540164C116834253 @default.
• W1593540164 hasConceptScore W1593540164C121332964 @default.
• W1593540164 hasConceptScore W1593540164C1276947 @default.
• W1593540164 hasConceptScore W1593540164C167625842 @default.
• W1593540164 hasConceptScore W1593540164C181199279 @default.
• W1593540164 hasConceptScore W1593540164C185592680 @default.
• W1593540164 hasConceptScore W1593540164C189909181 @default.
• W1593540164 hasConceptScore W1593540164C20251656 @default.
• W1593540164 hasConceptScore W1593540164C2776970464 @default.
• W1593540164 hasConceptScore W1593540164C2777977315 @default.
• W1593540164 hasConceptScore W1593540164C2778112365 @default.
• W1593540164 hasConceptScore W1593540164C2780854706 @default.
• W1593540164 hasConceptScore W1593540164C55493867 @default.
• W1593540164 hasConceptScore W1593540164C58431802 @default.
• W1593540164 hasConceptScore W1593540164C59822182 @default.
• W1593540164 hasConceptScore W1593540164C86803240 @default.
• W1593540164 hasConceptScore W1593540164C92825506 @default.
• W1593540164 hasIssue "24" @default.
• W1593540164 hasLocation W15935401641 @default.
• W1593540164 hasOpenAccess W1593540164 @default.
• W1593540164 hasPrimaryLocation W15935401641 @default.
• W1593540164 hasRelatedWork W1985772006 @default.
• W1593540164 hasRelatedWork W1985958494 @default.
• W1593540164 hasRelatedWork W2013706919 @default.
• W1593540164 hasRelatedWork W2048642571 @default.
• W1593540164 hasRelatedWork W2069966822 @default.
• W1593540164 hasRelatedWork W2149568310 @default.
• W1593540164 hasRelatedWork W2165167785 @default.
• W1593540164 hasRelatedWork W2258213298 @default.
• W1593540164 hasRelatedWork W2329822086 @default.
• W1593540164 hasRelatedWork W4249033680 @default.
• W1593540164 hasVolume "262" @default.
• W1593540164 isParatext "false" @default.
• W1593540164 isRetracted "false" @default.
• W1593540164 magId "1593540164" @default.

• next