FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1594133158> ?p ?o ?g. }

• W1594133158 endingPage "689" @default.
• W1594133158 startingPage "681" @default.
• W1594133158 abstract "Ras-like GTPases contain a structurally conserved GTP-binding domain. An important element of the GTP-binding domain is the phosphate-binding loop, which contains two Gly residues (Gly(12) and Gly(13)) in Ras. Because the two Gly residues are crucial for normal Ras function, it is intriguing that they are not conserved in other Ras-like GTPases, including the Rab GTPases; for example, the equivalent residues in Rab5 are Ser(29) and Ala(30). The present study builds on earlier biochemical characterizations of the Rab5 mutants containing substitutions at Ala(30) and provides a comprehensive analysis of the structure-function relationship of the Rab5 phosphate-binding loop. We have generated 19 new mutants containing amino acid substitutions at Ser(29) and determined whether these Ser(29) mutants, as well as the Ala(30) mutants, remain able to stimulate the endocytosis of horseradish peroxidase in baby hamster kidney cells. A total of 11 mutants lose the activity of stimulating endocytosis. Of these 11 mutants, 9 are defective in membrane association. In contrast, 27 mutants remain able to stimulate endocytosis. Five of them induce a novel cellular phenotype: cell rounding and detachment from culture dishes. They also induce super-large early endosomes such as the constitutively activated Rab5:Q79L mutant. Biochemical results suggest that the constitutive activation of Rab5 requires an increased nucleotide exchange rate and/or decreased GTPase activity. This study establishes functional significance for the phosphate-binding loop of Rab5 and shows that mutations in this region lead to either a loss-of-function or a gain-of-function phenotype, indicating a structure-function relationship distinct from that of Ras." @default.
• W1594133158 created "2016-06-24" @default.
• W1594133158 creator A5036114336 @default.
• W1594133158 creator A5091892806 @default.
• W1594133158 date "2001-04-24" @default.
• W1594133158 modified "2023-09-27" @default.
• W1594133158 title "Phosphate-binding loop and Rab GTPase function: mutations at Ser29 and Ala30 of Rab5 lead to loss-of-function as well as gain-of-function phenotype" @default.
• W1594133158 cites W1483127372 @default.
• W1594133158 cites W1486436311 @default.
• W1594133158 cites W1494370604 @default.
• W1594133158 cites W1505669099 @default.
• W1594133158 cites W1520904574 @default.
• W1594133158 cites W1563901359 @default.
• W1594133158 cites W1600485881 @default.
• W1594133158 cites W1606338704 @default.
• W1594133158 cites W1979527189 @default.
• W1594133158 cites W1992864103 @default.
• W1594133158 cites W2004362675 @default.
• W1594133158 cites W2005189737 @default.
• W1594133158 cites W2005574904 @default.
• W1594133158 cites W2007797957 @default.
• W1594133158 cites W2021510906 @default.
• W1594133158 cites W2034403065 @default.
• W1594133158 cites W2036277985 @default.
• W1594133158 cites W2043115431 @default.
• W1594133158 cites W2047137800 @default.
• W1594133158 cites W2047201925 @default.
• W1594133158 cites W2055747785 @default.
• W1594133158 cites W2074851426 @default.
• W1594133158 cites W2076926699 @default.
• W1594133158 cites W2078846166 @default.
• W1594133158 cites W2079095220 @default.
• W1594133158 cites W2086798592 @default.
• W1594133158 cites W2088000250 @default.
• W1594133158 cites W2096942986 @default.
• W1594133158 cites W2118434776 @default.
• W1594133158 cites W2121337367 @default.
• W1594133158 cites W2126162952 @default.
• W1594133158 cites W2140752038 @default.
• W1594133158 cites W2152561142 @default.
• W1594133158 cites W2159594827 @default.
• W1594133158 cites W2173344695 @default.
• W1594133158 cites W2436690735 @default.
• W1594133158 cites W4232777855 @default.
• W1594133158 doi "https://doi.org/10.1042/bj3550681" @default.
• W1594133158 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1221783" @default.
• W1594133158 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11311130" @default.
• W1594133158 hasPublicationYear "2001" @default.
• W1594133158 type Work @default.
• W1594133158 sameAs 1594133158 @default.
• W1594133158 citedByCount "30" @default.
• W1594133158 countsByYear W15941331582013 @default.
• W1594133158 countsByYear W15941331582015 @default.
• W1594133158 countsByYear W15941331582016 @default.
• W1594133158 countsByYear W15941331582018 @default.
• W1594133158 crossrefType "journal-article" @default.
• W1594133158 hasAuthorship W1594133158A5036114336 @default.
• W1594133158 hasAuthorship W1594133158A5091892806 @default.
• W1594133158 hasBestOaLocation W15941331582 @default.
• W1594133158 hasConcept C102747710 @default.
• W1594133158 hasConcept C104317684 @default.
• W1594133158 hasConcept C118716 @default.
• W1594133158 hasConcept C143065580 @default.
• W1594133158 hasConcept C156728348 @default.
• W1594133158 hasConcept C170493617 @default.
• W1594133158 hasConcept C181199279 @default.
• W1594133158 hasConcept C207332259 @default.
• W1594133158 hasConcept C2780298669 @default.
• W1594133158 hasConcept C28005876 @default.
• W1594133158 hasConcept C501734568 @default.
• W1594133158 hasConcept C55493867 @default.
• W1594133158 hasConcept C62478195 @default.
• W1594133158 hasConcept C86803240 @default.
• W1594133158 hasConcept C95444343 @default.
• W1594133158 hasConceptScore W1594133158C102747710 @default.
• W1594133158 hasConceptScore W1594133158C104317684 @default.
• W1594133158 hasConceptScore W1594133158C118716 @default.
• W1594133158 hasConceptScore W1594133158C143065580 @default.
• W1594133158 hasConceptScore W1594133158C156728348 @default.
• W1594133158 hasConceptScore W1594133158C170493617 @default.
• W1594133158 hasConceptScore W1594133158C181199279 @default.
• W1594133158 hasConceptScore W1594133158C207332259 @default.
• W1594133158 hasConceptScore W1594133158C2780298669 @default.
• W1594133158 hasConceptScore W1594133158C28005876 @default.
• W1594133158 hasConceptScore W1594133158C501734568 @default.
• W1594133158 hasConceptScore W1594133158C55493867 @default.
• W1594133158 hasConceptScore W1594133158C62478195 @default.
• W1594133158 hasConceptScore W1594133158C86803240 @default.
• W1594133158 hasConceptScore W1594133158C95444343 @default.
• W1594133158 hasIssue "3" @default.
• W1594133158 hasLocation W15941331581 @default.
• W1594133158 hasLocation W15941331582 @default.
• W1594133158 hasLocation W15941331583 @default.
• W1594133158 hasLocation W15941331584 @default.
• W1594133158 hasOpenAccess W1594133158 @default.
• W1594133158 hasPrimaryLocation W15941331581 @default.
• W1594133158 hasRelatedWork W2012653610 @default.
• W1594133158 hasRelatedWork W2030210725 @default.

• next