FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1594220199> ?p ?o ?g. }

• W1594220199 endingPage "14838" @default.
• W1594220199 startingPage "14832" @default.
• W1594220199 abstract "To identify a receptor binding site of human interleukin-6 (IL-6), we created a library of IL-6 variants with single amino acid substitutions in the last 15 residues (171-185) in the COOH terminus of IL-6. Twenty-seven IL-6 variants were tested for biological activity on a human hepatoma and a mouse hybridoma cell line. Most variants were additionally tested in a receptor binding assay using a human myeloma cell line. Several single amino acid substitutions in the COOH terminus of IL-6 were found to decrease biological activity significantly. This is especially seen in variants with amino acid substitutions that alter the postulated amphipathical alpha-helix structure between residues 178 and 183. The two highly conserved Arg residues at positions 180 and 183 seem to play a very important role in biological activity. The loss of biological activity in all inactive variants is completely paralleled by a decrease of IL-6 receptor binding, as determined by competition binding experiments. One mutant (Leu171) displayed a higher activity on human cells and a higher binding affinity to the receptor and can be considered an IL-6 agonist. It is concluded that the amphipathical alpha-helix structure in the COOH terminus of IL-6 is critical for ligand receptor interaction. Furthermore, the region between residues Ser178 and Arg183 (Ser-Leu-Arg-Ala-X-Arg) is identified as a receptor binding site in the COOH terminus of human IL-6." @default.
• W1594220199 created "2016-06-24" @default.
• W1594220199 creator A5002870795 @default.
• W1594220199 creator A5055855475 @default.
• W1594220199 creator A5059655767 @default.
• W1594220199 creator A5082076726 @default.
• W1594220199 date "1992-07-01" @default.
• W1594220199 modified "2023-10-18" @default.
• W1594220199 title "Identification of a receptor binding site in the carboxyl terminus of human interleukin-6." @default.
• W1594220199 cites W134083461 @default.
• W1594220199 cites W1497376115 @default.
• W1594220199 cites W1499539788 @default.
• W1594220199 cites W1574330564 @default.
• W1594220199 cites W1607821849 @default.
• W1594220199 cites W1621840684 @default.
• W1594220199 cites W1838497045 @default.
• W1594220199 cites W1968295792 @default.
• W1594220199 cites W1969408920 @default.
• W1594220199 cites W1972155218 @default.
• W1594220199 cites W1974486772 @default.
• W1594220199 cites W1982134009 @default.
• W1594220199 cites W1983224547 @default.
• W1594220199 cites W2002076761 @default.
• W1594220199 cites W2007000374 @default.
• W1594220199 cites W2022881613 @default.
• W1594220199 cites W2029128430 @default.
• W1594220199 cites W2031086429 @default.
• W1594220199 cites W2032118018 @default.
• W1594220199 cites W2036452124 @default.
• W1594220199 cites W2042655455 @default.
• W1594220199 cites W2043813981 @default.
• W1594220199 cites W2052293758 @default.
• W1594220199 cites W2054663403 @default.
• W1594220199 cites W2056502094 @default.
• W1594220199 cites W2061628667 @default.
• W1594220199 cites W2064484362 @default.
• W1594220199 cites W2067377498 @default.
• W1594220199 cites W2068187158 @default.
• W1594220199 cites W2085898638 @default.
• W1594220199 cites W2088865336 @default.
• W1594220199 cites W2106002718 @default.
• W1594220199 cites W2106178962 @default.
• W1594220199 cites W2112916314 @default.
• W1594220199 cites W2112944055 @default.
• W1594220199 cites W2114471659 @default.
• W1594220199 cites W2120056767 @default.
• W1594220199 cites W2129127560 @default.
• W1594220199 cites W2135392289 @default.
• W1594220199 cites W2149419600 @default.
• W1594220199 cites W2151195343 @default.
• W1594220199 cites W2162428378 @default.
• W1594220199 cites W2168059460 @default.
• W1594220199 cites W2169046469 @default.
• W1594220199 cites W2211545673 @default.
• W1594220199 cites W2261358491 @default.
• W1594220199 cites W2418064775 @default.
• W1594220199 cites W4245868295 @default.
• W1594220199 doi "https://doi.org/10.1016/s0021-9258(18)42115-1" @default.
• W1594220199 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1321818" @default.
• W1594220199 hasPublicationYear "1992" @default.
• W1594220199 type Work @default.
• W1594220199 sameAs 1594220199 @default.
• W1594220199 citedByCount "55" @default.
• W1594220199 countsByYear W15942201992020 @default.
• W1594220199 crossrefType "journal-article" @default.
• W1594220199 hasAuthorship W1594220199A5002870795 @default.
• W1594220199 hasAuthorship W1594220199A5055855475 @default.
• W1594220199 hasAuthorship W1594220199A5059655767 @default.
• W1594220199 hasAuthorship W1594220199A5082076726 @default.
• W1594220199 hasBestOaLocation W15942201991 @default.
• W1594220199 hasConcept C107824862 @default.
• W1594220199 hasConcept C116834253 @default.
• W1594220199 hasConcept C170493617 @default.
• W1594220199 hasConcept C185592680 @default.
• W1594220199 hasConcept C18903297 @default.
• W1594220199 hasConcept C55493867 @default.
• W1594220199 hasConcept C70721500 @default.
• W1594220199 hasConcept C86803240 @default.
• W1594220199 hasConceptScore W1594220199C107824862 @default.
• W1594220199 hasConceptScore W1594220199C116834253 @default.
• W1594220199 hasConceptScore W1594220199C170493617 @default.
• W1594220199 hasConceptScore W1594220199C185592680 @default.
• W1594220199 hasConceptScore W1594220199C18903297 @default.
• W1594220199 hasConceptScore W1594220199C55493867 @default.
• W1594220199 hasConceptScore W1594220199C70721500 @default.
• W1594220199 hasConceptScore W1594220199C86803240 @default.
• W1594220199 hasIssue "21" @default.
• W1594220199 hasLocation W15942201991 @default.
• W1594220199 hasLocation W15942201992 @default.
• W1594220199 hasOpenAccess W1594220199 @default.
• W1594220199 hasPrimaryLocation W15942201991 @default.
• W1594220199 hasRelatedWork W1124181109 @default.
• W1594220199 hasRelatedWork W1972473893 @default.
• W1594220199 hasRelatedWork W2188270127 @default.
• W1594220199 hasRelatedWork W2383658677 @default.
• W1594220199 hasRelatedWork W2410053581 @default.
• W1594220199 hasRelatedWork W2466435674 @default.
• W1594220199 hasRelatedWork W2898732673 @default.

• next