Matches in SemOpenAlex for { <https://semopenalex.org/work/W1594388775> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1594388775 endingPage "2911" @default.
- W1594388775 startingPage "2906" @default.
- W1594388775 abstract "AIM: To isolate nestin-positive progenitor cells from human fetal pancreas and to detect their surface markers and their capability of proliferation and differentiation into pancreatic islet endocrine cells in vitro.METHODS: Islet-like cell clusters (ICCs) were isolated from human fetal pancreas by using collagenase digestion. The free-floating ICCs were handpicked and cultured in a new dish. After the ICCs developed into monolayer epithelium-like cells, they were passaged and induced for differentiation. Reverse transcription polymerase chain reaction (RT-PCR), immunofluorescence stain, fluorescenceactivated cell sorting (FACS) and radioimmunoassay (RIA)were used to detect the expression of cell markers. RESULTS: (1) The monolayer epithelium-like cells had highly proliferative potential and could be passaged more than 16 timesin vitro; (2) RT-PCR analysis and immunofluorescence stain showed that these cells expressed both nestin and ABCG2, two of stem cellmarkers; (3) FACS analysis revealed that CD44, CD90and CD147 were positive, whereas CD34, CD38, CD45, CD71, CD117, CD133 and HLA-DR were negative on the nestin-positive cells; (4) RT-PCR analysis showed that the mRNA expression of insulin, glucagon and pancreaticduodenal homeobox gene-1 was detected, whereas the expression of nestin and neurogenin 3 disappeared in these cells treated with serum-free media supplemented with the cocktail of growth factors. Furthermore, the intracellular insulin content was detected by RIA after the induction culture.CONCLUSION: Nestin-positive cells isolated from human fetal pancreas possess the characteristics of pancreatic progenitor cells since they have highly proliferative potential and the capability of differentiation into insulinproducing cells in vitro. Interestingly, the nestin-positive pancreatic progenitor cells share many phenotypic markers with mesenchymal stem cells derived from bone marrow." @default.
- W1594388775 created "2016-06-24" @default.
- W1594388775 creator A5010219762 @default.
- W1594388775 creator A5028919904 @default.
- W1594388775 creator A5045171906 @default.
- W1594388775 creator A5053268819 @default.
- W1594388775 creator A5068743916 @default.
- W1594388775 creator A5084135237 @default.
- W1594388775 date "2005-01-01" @default.
- W1594388775 modified "2023-09-23" @default.
- W1594388775 title "Nestin-positive progenitor cells isolated from human fetal pancreas have phenotypic markers identical to mesenchymal stem cells" @default.
- W1594388775 hasPublicationYear "2005" @default.
- W1594388775 type Work @default.
- W1594388775 sameAs 1594388775 @default.
- W1594388775 citedByCount "0" @default.
- W1594388775 crossrefType "journal-article" @default.
- W1594388775 hasAuthorship W1594388775A5010219762 @default.
- W1594388775 hasAuthorship W1594388775A5028919904 @default.
- W1594388775 hasAuthorship W1594388775A5045171906 @default.
- W1594388775 hasAuthorship W1594388775A5053268819 @default.
- W1594388775 hasAuthorship W1594388775A5068743916 @default.
- W1594388775 hasAuthorship W1594388775A5084135237 @default.
- W1594388775 hasConcept C10205521 @default.
- W1594388775 hasConcept C104317684 @default.
- W1594388775 hasConcept C134018914 @default.
- W1594388775 hasConcept C136302470 @default.
- W1594388775 hasConcept C136834591 @default.
- W1594388775 hasConcept C148738053 @default.
- W1594388775 hasConcept C153911025 @default.
- W1594388775 hasConcept C165220095 @default.
- W1594388775 hasConcept C198826908 @default.
- W1594388775 hasConcept C201750760 @default.
- W1594388775 hasConcept C202751555 @default.
- W1594388775 hasConcept C2777738006 @default.
- W1594388775 hasConcept C2778764654 @default.
- W1594388775 hasConcept C2779050171 @default.
- W1594388775 hasConcept C2779306644 @default.
- W1594388775 hasConcept C2780932548 @default.
- W1594388775 hasConcept C28328180 @default.
- W1594388775 hasConcept C55493867 @default.
- W1594388775 hasConcept C86803240 @default.
- W1594388775 hasConcept C95444343 @default.
- W1594388775 hasConceptScore W1594388775C10205521 @default.
- W1594388775 hasConceptScore W1594388775C104317684 @default.
- W1594388775 hasConceptScore W1594388775C134018914 @default.
- W1594388775 hasConceptScore W1594388775C136302470 @default.
- W1594388775 hasConceptScore W1594388775C136834591 @default.
- W1594388775 hasConceptScore W1594388775C148738053 @default.
- W1594388775 hasConceptScore W1594388775C153911025 @default.
- W1594388775 hasConceptScore W1594388775C165220095 @default.
- W1594388775 hasConceptScore W1594388775C198826908 @default.
- W1594388775 hasConceptScore W1594388775C201750760 @default.
- W1594388775 hasConceptScore W1594388775C202751555 @default.
- W1594388775 hasConceptScore W1594388775C2777738006 @default.
- W1594388775 hasConceptScore W1594388775C2778764654 @default.
- W1594388775 hasConceptScore W1594388775C2779050171 @default.
- W1594388775 hasConceptScore W1594388775C2779306644 @default.
- W1594388775 hasConceptScore W1594388775C2780932548 @default.
- W1594388775 hasConceptScore W1594388775C28328180 @default.
- W1594388775 hasConceptScore W1594388775C55493867 @default.
- W1594388775 hasConceptScore W1594388775C86803240 @default.
- W1594388775 hasConceptScore W1594388775C95444343 @default.
- W1594388775 hasIssue "19" @default.
- W1594388775 hasLocation W15943887751 @default.
- W1594388775 hasOpenAccess W1594388775 @default.
- W1594388775 hasPrimaryLocation W15943887751 @default.
- W1594388775 hasRelatedWork W1984316169 @default.
- W1594388775 hasRelatedWork W2106677068 @default.
- W1594388775 hasRelatedWork W2109362768 @default.
- W1594388775 hasRelatedWork W2168448946 @default.
- W1594388775 hasRelatedWork W2186222783 @default.
- W1594388775 hasRelatedWork W2349680009 @default.
- W1594388775 hasRelatedWork W2351552098 @default.
- W1594388775 hasRelatedWork W2356101126 @default.
- W1594388775 hasRelatedWork W2358234797 @default.
- W1594388775 hasRelatedWork W2360245945 @default.
- W1594388775 hasRelatedWork W2361535431 @default.
- W1594388775 hasRelatedWork W2364775531 @default.
- W1594388775 hasRelatedWork W2365822793 @default.
- W1594388775 hasRelatedWork W2366033602 @default.
- W1594388775 hasRelatedWork W2368133661 @default.
- W1594388775 hasRelatedWork W2375649508 @default.
- W1594388775 hasRelatedWork W2381845242 @default.
- W1594388775 hasRelatedWork W2383704240 @default.
- W1594388775 hasRelatedWork W2384769194 @default.
- W1594388775 hasRelatedWork W2462487990 @default.
- W1594388775 hasVolume "11" @default.
- W1594388775 isParatext "false" @default.
- W1594388775 isRetracted "false" @default.
- W1594388775 magId "1594388775" @default.
- W1594388775 workType "article" @default.