Matches in SemOpenAlex for { <https://semopenalex.org/work/W1594420006> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1594420006 endingPage "10763" @default.
- W1594420006 startingPage "10760" @default.
- W1594420006 abstract "The restriction endonuclease EcoRI hydrolyzes the Rp diastereomer of d(pGGsAATTCC), an analogue of d(pGGAATTCC) containing a chiral phosphorothioate group at the cleavage site between the deoxyguanosine and the deoxyadenosine residues (Connolly, B.A., Potter, B.V.L., Eckstein, F., Pingoud, A., and Grotjahn, L. (1984) Biochemistry 23, 3343-3453). Performing the reaction in H2(18)O leads to d(pGG) and the hexanucleotide d([18O, S]pAATTCC) which has an 18O-containing phosphorothioate group at the 5' terminus. Further hydrolysis of this hexamer with nuclease P1 yields deoxyadenosine 5'-O-[18O]phosphorothioate which can be stereospecifically phosphorylated with adenylate kinase and pyruvate kinase to give Sp-[18O] deoxyadenosine 5'-O-(1-thiotriphosphate). 31P NMR spectroscopy shows the oxygen-18 in this compound to be in a bridging position between the alpha- and beta-phosphorus atoms. Thus, the hydrolysis reaction catalyzed by EcoRI proceeds with inversion of configuration at phosphorus. This result is compatible with a direct enzyme-catalyzed nucleophilic attack of H2O at phosphorus without involvement of a covalent enzyme intermediate." @default.
- W1594420006 created "2016-06-24" @default.
- W1594420006 creator A5014782626 @default.
- W1594420006 creator A5059665923 @default.
- W1594420006 creator A5088616795 @default.
- W1594420006 date "1984-09-01" @default.
- W1594420006 modified "2023-10-15" @default.
- W1594420006 title "The stereochemical course of the restriction endonuclease EcoRI-catalyzed reaction." @default.
- W1594420006 cites W1124885369 @default.
- W1594420006 cites W1493189267 @default.
- W1594420006 cites W1508603686 @default.
- W1594420006 cites W1535056737 @default.
- W1594420006 cites W1568293155 @default.
- W1594420006 cites W1573005391 @default.
- W1594420006 cites W1648852411 @default.
- W1594420006 cites W1966492644 @default.
- W1594420006 cites W1967625504 @default.
- W1594420006 cites W1968659514 @default.
- W1594420006 cites W1975497571 @default.
- W1594420006 cites W1992445837 @default.
- W1594420006 cites W2012738612 @default.
- W1594420006 cites W2029312735 @default.
- W1594420006 cites W2034278917 @default.
- W1594420006 cites W2034289839 @default.
- W1594420006 cites W2037598744 @default.
- W1594420006 cites W2062823420 @default.
- W1594420006 cites W2067031652 @default.
- W1594420006 cites W2069766218 @default.
- W1594420006 cites W2074325382 @default.
- W1594420006 cites W2079043642 @default.
- W1594420006 cites W2083125019 @default.
- W1594420006 cites W2151958020 @default.
- W1594420006 cites W2153630542 @default.
- W1594420006 cites W2158496968 @default.
- W1594420006 cites W65538786 @default.
- W1594420006 doi "https://doi.org/10.1016/s0021-9258(18)90576-4" @default.
- W1594420006 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6088516" @default.
- W1594420006 hasPublicationYear "1984" @default.
- W1594420006 type Work @default.
- W1594420006 sameAs 1594420006 @default.
- W1594420006 citedByCount "90" @default.
- W1594420006 countsByYear W15944200062013 @default.
- W1594420006 countsByYear W15944200062014 @default.
- W1594420006 countsByYear W15944200062015 @default.
- W1594420006 countsByYear W15944200062021 @default.
- W1594420006 crossrefType "journal-article" @default.
- W1594420006 hasAuthorship W1594420006A5014782626 @default.
- W1594420006 hasAuthorship W1594420006A5059665923 @default.
- W1594420006 hasAuthorship W1594420006A5088616795 @default.
- W1594420006 hasBestOaLocation W15944200061 @default.
- W1594420006 hasConcept C128319531 @default.
- W1594420006 hasConcept C130123407 @default.
- W1594420006 hasConcept C161790260 @default.
- W1594420006 hasConcept C185592680 @default.
- W1594420006 hasConcept C552990157 @default.
- W1594420006 hasConcept C55493867 @default.
- W1594420006 hasConcept C71240020 @default.
- W1594420006 hasConcept C86803240 @default.
- W1594420006 hasConceptScore W1594420006C128319531 @default.
- W1594420006 hasConceptScore W1594420006C130123407 @default.
- W1594420006 hasConceptScore W1594420006C161790260 @default.
- W1594420006 hasConceptScore W1594420006C185592680 @default.
- W1594420006 hasConceptScore W1594420006C552990157 @default.
- W1594420006 hasConceptScore W1594420006C55493867 @default.
- W1594420006 hasConceptScore W1594420006C71240020 @default.
- W1594420006 hasConceptScore W1594420006C86803240 @default.
- W1594420006 hasIssue "17" @default.
- W1594420006 hasLocation W15944200061 @default.
- W1594420006 hasOpenAccess W1594420006 @default.
- W1594420006 hasPrimaryLocation W15944200061 @default.
- W1594420006 hasRelatedWork W1971494494 @default.
- W1594420006 hasRelatedWork W1981054976 @default.
- W1594420006 hasRelatedWork W1998774522 @default.
- W1594420006 hasRelatedWork W2090243771 @default.
- W1594420006 hasRelatedWork W2154296547 @default.
- W1594420006 hasRelatedWork W2373469313 @default.
- W1594420006 hasRelatedWork W2395723962 @default.
- W1594420006 hasRelatedWork W2410954609 @default.
- W1594420006 hasRelatedWork W2413723606 @default.
- W1594420006 hasRelatedWork W2790413672 @default.
- W1594420006 hasVolume "259" @default.
- W1594420006 isParatext "false" @default.
- W1594420006 isRetracted "false" @default.
- W1594420006 magId "1594420006" @default.
- W1594420006 workType "article" @default.