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- W1594445496 abstract "A solid-phase radioimmunoassay employing 125 I-labeled enterotoxins and polystyrene tubes coated with specific antibody has been developed for assaying the relative concentrations of antibodies to staphylococcal enterotoxins A and B. Competitive binding occurs between tube-bound antibody and free antibody for binding sites on 125 I-labeled enterotoxin. The sensitivity of the system is affected by the amount of antibody on the walls of the tubes, the concentration of 125 I-labeled enterotoxin added to the system, and probably by the relative binding affinities of the bound and unbound antibodies. Antibody, 0.01 to 0.07 μg/ml, inhibited the uptake of 125 I-labeled enterotoxin by 20%. Both the antibody and antigen solid-phase radioimmunoassay inhibition systems can be appropriately represented by either of the following two models: Log e ( Y /1 - Y ) = α 0 + α 1 Log e X and Log e Y = β 0 + β 1 Log e X , where Y is bound activity, X is antigen or antibody concentration for inhibition, and α 0 , α 1 , β 0 , and β 1 are regression coefficients. Estimates from the first model were slightly more precise for the antibody system, whereas the reverse was true for the antigen system." @default.
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- W1594445496 date "1975-06-01" @default.
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- W1594445496 title "Comparative Inhibitory Effects of Antigen and Antibody in the Staphylococcal Enterotoxin Solid-Phase Radioimmunoassay System" @default.
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- W1594445496 doi "https://doi.org/10.1128/am.29.6.765-768.1975" @default.
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