Matches in SemOpenAlex for { <https://semopenalex.org/work/W1594512099> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W1594512099 abstract "Abstract : This project proposes the development of a recombinant polyclonal antibody specific for the human breast carcinoma cell line BT-20. The proposed library would target multiple epitopes on the human cell line and also provide the genes necessary for the unlimited perpetuation of the defined antibody mixture. Ultimately, such antibody libraries could be used for treatment and diagnosis of breast cancer in humans. In the present study, V region genes for construction of a polyclonal Fab phage display library were obtained from the spleen, bone marrow, and intestine of a BALB/c mouse that had been immunized with BT-20 cells. The light and heavy chain V region genes were linked by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into a bidirectional phagemid vector to express an Fab-displaying phage library. This library was then selected for binding to BT-20 cells. Direct and inhibition ELISA showed that the selected Fab phage display library bound specifically to BT-20 cells, and that this binding could be inhibited by BT-20 as well as by other human breast cancer cell lines, but not by sheep red blood cells. These results demonstrate that the first phase of generating a polyclonal antibody library can be done successfully." @default.
- W1594512099 created "2016-06-24" @default.
- W1594512099 creator A5003615763 @default.
- W1594512099 date "2000-09-01" @default.
- W1594512099 modified "2023-09-24" @default.
- W1594512099 title "Recombinant Polyclonal Antibody Libraries for Breast Cancer Therapy" @default.
- W1594512099 cites W1558813989 @default.
- W1594512099 cites W1569093699 @default.
- W1594512099 cites W570995313 @default.
- W1594512099 doi "https://doi.org/10.21236/ada397043" @default.
- W1594512099 hasPublicationYear "2000" @default.
- W1594512099 type Work @default.
- W1594512099 sameAs 1594512099 @default.
- W1594512099 citedByCount "0" @default.
- W1594512099 crossrefType "report" @default.
- W1594512099 hasAuthorship W1594512099A5003615763 @default.
- W1594512099 hasConcept C10079403 @default.
- W1594512099 hasConcept C104317684 @default.
- W1594512099 hasConcept C153911025 @default.
- W1594512099 hasConcept C159047783 @default.
- W1594512099 hasConcept C159654299 @default.
- W1594512099 hasConcept C186268636 @default.
- W1594512099 hasConcept C195616568 @default.
- W1594512099 hasConcept C203014093 @default.
- W1594512099 hasConcept C2776441376 @default.
- W1594512099 hasConcept C40767141 @default.
- W1594512099 hasConcept C54355233 @default.
- W1594512099 hasConcept C547475151 @default.
- W1594512099 hasConcept C86803240 @default.
- W1594512099 hasConcept C956191 @default.
- W1594512099 hasConceptScore W1594512099C10079403 @default.
- W1594512099 hasConceptScore W1594512099C104317684 @default.
- W1594512099 hasConceptScore W1594512099C153911025 @default.
- W1594512099 hasConceptScore W1594512099C159047783 @default.
- W1594512099 hasConceptScore W1594512099C159654299 @default.
- W1594512099 hasConceptScore W1594512099C186268636 @default.
- W1594512099 hasConceptScore W1594512099C195616568 @default.
- W1594512099 hasConceptScore W1594512099C203014093 @default.
- W1594512099 hasConceptScore W1594512099C2776441376 @default.
- W1594512099 hasConceptScore W1594512099C40767141 @default.
- W1594512099 hasConceptScore W1594512099C54355233 @default.
- W1594512099 hasConceptScore W1594512099C547475151 @default.
- W1594512099 hasConceptScore W1594512099C86803240 @default.
- W1594512099 hasConceptScore W1594512099C956191 @default.
- W1594512099 hasLocation W15945120991 @default.
- W1594512099 hasOpenAccess W1594512099 @default.
- W1594512099 hasPrimaryLocation W15945120991 @default.
- W1594512099 hasRelatedWork W1544920924 @default.
- W1594512099 hasRelatedWork W2052543396 @default.
- W1594512099 hasRelatedWork W2063544229 @default.
- W1594512099 hasRelatedWork W2089821475 @default.
- W1594512099 hasRelatedWork W2151850182 @default.
- W1594512099 hasRelatedWork W2156023245 @default.
- W1594512099 hasRelatedWork W2282704278 @default.
- W1594512099 hasRelatedWork W2351354637 @default.
- W1594512099 hasRelatedWork W2365463527 @default.
- W1594512099 hasRelatedWork W2367322708 @default.
- W1594512099 hasRelatedWork W2368365144 @default.
- W1594512099 hasRelatedWork W2379848273 @default.
- W1594512099 hasRelatedWork W2380138866 @default.
- W1594512099 hasRelatedWork W2390423144 @default.
- W1594512099 hasRelatedWork W2393662679 @default.
- W1594512099 hasRelatedWork W2393971010 @default.
- W1594512099 hasRelatedWork W2397789451 @default.
- W1594512099 hasRelatedWork W2767993154 @default.
- W1594512099 hasRelatedWork W3160348301 @default.
- W1594512099 hasRelatedWork W41802117 @default.
- W1594512099 isParatext "false" @default.
- W1594512099 isRetracted "false" @default.
- W1594512099 magId "1594512099" @default.
- W1594512099 workType "report" @default.