Matches in SemOpenAlex for { <https://semopenalex.org/work/W1594588909> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W1594588909 endingPage "8" @default.
- W1594588909 startingPage "4453" @default.
- W1594588909 abstract "The genetic suppressor element (GSE) approach allows identification of genes essential for certain cell phenotypes. To identify genes controlling the cell response to cytotoxic agents, a normalized retroviral library of randomly fragmented cDNAs from the Chinese hamster cell line DC-3F was screened for GSEs conferring resistance to bleomycin. One of these GSEs, GSE(BLM), conferring an approximately 2-fold bleomycin resistance in DC-3F cells, displayed 98% identity with an amino acid sequence located in the functional domain of human SRPK1. Using GSE(BLM) as a probe, we cloned a cDNA with a nucleotide sequence that was 76.7% identical to that of human SRPK1, whereas the corresponding amino acid sequence was 92.6% identical to that of this enzyme. When GSE(BLM), inserted in the retroviral vector pLNCX, was transduced in HeLa cells, its expression resulted in a 5-10-fold bleomycin resistance, which was abolished when these cells were further transfected with SRPK1 cDNA. In our experimental conditions, DC-3F or HeLa cells expressing GSE(BLM) did not show any detectable cross-resistance to other cytotoxic agents with various mechanisms of action. GSE(BLM), which is sense oriented in the vector, is likely to be translated in a peptide active as a dominant-negative inhibitor of SRPK1. SRPK1 is a protein serine kinase that regulates the activity of RS-proteins (arginine-serine-rich proteins), a group of nuclear factors controlling various physiological processes." @default.
- W1594588909 created "2016-06-24" @default.
- W1594588909 creator A5016864528 @default.
- W1594588909 creator A5038021150 @default.
- W1594588909 creator A5061987521 @default.
- W1594588909 date "2002-08-01" @default.
- W1594588909 modified "2023-09-25" @default.
- W1594588909 title "Bleomycin resistance in mammalian cells expressing a genetic suppressor element derived from the SRPK1 gene." @default.
- W1594588909 cites W1489777708 @default.
- W1594588909 cites W1521817136 @default.
- W1594588909 cites W1601850580 @default.
- W1594588909 cites W1970805100 @default.
- W1594588909 cites W1978438460 @default.
- W1594588909 cites W1982745217 @default.
- W1594588909 cites W1987698463 @default.
- W1594588909 cites W1996371649 @default.
- W1594588909 cites W2025537059 @default.
- W1594588909 cites W2026623097 @default.
- W1594588909 cites W2028272667 @default.
- W1594588909 cites W2033516066 @default.
- W1594588909 cites W2046013336 @default.
- W1594588909 cites W2053336269 @default.
- W1594588909 cites W2060618478 @default.
- W1594588909 cites W2077729815 @default.
- W1594588909 cites W2080568795 @default.
- W1594588909 cites W2110224941 @default.
- W1594588909 cites W2111409695 @default.
- W1594588909 cites W2113529168 @default.
- W1594588909 cites W2125097639 @default.
- W1594588909 cites W2142122497 @default.
- W1594588909 cites W2154153541 @default.
- W1594588909 cites W2159611559 @default.
- W1594588909 cites W2161358134 @default.
- W1594588909 cites W2419359951 @default.
- W1594588909 cites W2429392527 @default.
- W1594588909 cites W3020979083 @default.
- W1594588909 cites W569951484 @default.
- W1594588909 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12154054" @default.
- W1594588909 hasPublicationYear "2002" @default.
- W1594588909 type Work @default.
- W1594588909 sameAs 1594588909 @default.
- W1594588909 citedByCount "7" @default.
- W1594588909 countsByYear W15945889092018 @default.
- W1594588909 crossrefType "journal-article" @default.
- W1594588909 hasAuthorship W1594588909A5016864528 @default.
- W1594588909 hasAuthorship W1594588909A5038021150 @default.
- W1594588909 hasAuthorship W1594588909A5061987521 @default.
- W1594588909 hasConcept C104317684 @default.
- W1594588909 hasConcept C153911025 @default.
- W1594588909 hasConcept C167625842 @default.
- W1594588909 hasConcept C181199279 @default.
- W1594588909 hasConcept C187882448 @default.
- W1594588909 hasConcept C2776232574 @default.
- W1594588909 hasConcept C2776414213 @default.
- W1594588909 hasConcept C2776694085 @default.
- W1594588909 hasConcept C2777366897 @default.
- W1594588909 hasConcept C54009773 @default.
- W1594588909 hasConcept C54355233 @default.
- W1594588909 hasConcept C55493867 @default.
- W1594588909 hasConcept C81885089 @default.
- W1594588909 hasConcept C86803240 @default.
- W1594588909 hasConceptScore W1594588909C104317684 @default.
- W1594588909 hasConceptScore W1594588909C153911025 @default.
- W1594588909 hasConceptScore W1594588909C167625842 @default.
- W1594588909 hasConceptScore W1594588909C181199279 @default.
- W1594588909 hasConceptScore W1594588909C187882448 @default.
- W1594588909 hasConceptScore W1594588909C2776232574 @default.
- W1594588909 hasConceptScore W1594588909C2776414213 @default.
- W1594588909 hasConceptScore W1594588909C2776694085 @default.
- W1594588909 hasConceptScore W1594588909C2777366897 @default.
- W1594588909 hasConceptScore W1594588909C54009773 @default.
- W1594588909 hasConceptScore W1594588909C54355233 @default.
- W1594588909 hasConceptScore W1594588909C55493867 @default.
- W1594588909 hasConceptScore W1594588909C81885089 @default.
- W1594588909 hasConceptScore W1594588909C86803240 @default.
- W1594588909 hasIssue "15" @default.
- W1594588909 hasLocation W15945889091 @default.
- W1594588909 hasLocation W15945889092 @default.
- W1594588909 hasLocation W15945889093 @default.
- W1594588909 hasOpenAccess W1594588909 @default.
- W1594588909 hasPrimaryLocation W15945889091 @default.
- W1594588909 hasRelatedWork W2349566129 @default.
- W1594588909 hasRelatedWork W2351114716 @default.
- W1594588909 hasRelatedWork W2352258017 @default.
- W1594588909 hasRelatedWork W2356648071 @default.
- W1594588909 hasRelatedWork W2358723974 @default.
- W1594588909 hasRelatedWork W2370939691 @default.
- W1594588909 hasRelatedWork W2376774677 @default.
- W1594588909 hasRelatedWork W2386240997 @default.
- W1594588909 hasRelatedWork W2416204398 @default.
- W1594588909 hasRelatedWork W3150194181 @default.
- W1594588909 hasVolume "62" @default.
- W1594588909 isParatext "false" @default.
- W1594588909 isRetracted "false" @default.
- W1594588909 magId "1594588909" @default.
- W1594588909 workType "article" @default.