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- W1594712198 abstract "A thymine-requiring mutant of Staphylococcus aureus, strain 8325 (PI258)thy, undergoes prophage induction and lysis after thymine starvation. Four different phages were isolated from the lysate in low titers, among which was a phage designated phi 14, which differs from phage phi 11 in its immunity locus. The thymineless induced lysates of strain 8325(PI258)thy transduce the penicillinase plasmid at high frequency (10(-1), whereas transduction of chromosomal markers is inefficient. A plasmic-cured derivative of strain 8325(PI258)thy is also lysed by thymine starvation and be used for high-frequency transduction of other plasmids. Reconstitution of a strain of S. aureus that responds to thymine starvation was only partially successful, but this system can effectively be used to transduce plasmids or plasmid derivatives." @default.
- W1594712198 created "2016-06-24" @default.
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- W1594712198 date "1975-12-01" @default.
- W1594712198 modified "2023-10-14" @default.
- W1594712198 title "Thymineless bacteriophage induction in Staphylococcus aureus. I. High-frequency transduction with lysates containing a bacteriophage related to bacteriophage phi 11" @default.
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- W1594712198 doi "https://doi.org/10.1128/jvi.16.6.1357-1366.1975" @default.
- W1594712198 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/355743" @default.
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