FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1594920269> ?p ?o ?g. }

• W1594920269 endingPage "3970" @default.
• W1594920269 startingPage "3965" @default.
• W1594920269 abstract "Abstract Anti-CD3 x anti-tumor-bispecific Abs have been used to redirect cytotoxic T cells to tumor cells in an MHC-unrestricted fashion and to induce their rejection in vivo. We have recently described a recombinant bispecific single-chain Ab that combines four different V regions of two Abs, anti-17-1A and anti-CD3, on one polypeptide chain. It folds correctly to a 60-kDa globular protein and is secreted in fully functional form by a high producer Chinese hamster ovary cell line. In this work, we report that its remarkable cytotoxicity against 17-1A+ tumor cells is exerted via T cells without an apparent engagement of a detectable costimulatory pathway. T cells are activated only by the bispecific Ab when coincubated with 17-1A+ target cells. In a chromium release assay, CD8+ T cells reach maximal tumor cell cytotoxicity within 4 h, while CD4+ T cells need about 20 h to reach similar levels of cytotoxicity. Addition of costimulatory CD28 Abs did not lead to a further increase in cytotoxicity. Its remarkable stability at 37 degrees in serum, the ease of production, and purification by affinity chromatography via polyhistidine tail make this smaller version of a bispecific Ab a promising candidate for a therapeutic trial in patients with solid tumor. Because adjuvant therapy with an intact, much less cytotoxic IgG2a Ab against the 17-1A target had already increased the 7-yr survival of colorectal cancer patients by 30%, the presented small bispecific construct lacking the immunogenic murine Fc region as well as autochthonous T lymphocyte stimulatory activity warrants a therapeutic trial in patients with minimal residual 17-1A+ cancer." @default.
• W1594920269 created "2016-06-24" @default.
• W1594920269 creator A5001528551 @default.
• W1594920269 creator A5008627097 @default.
• W1594920269 creator A5009261802 @default.
• W1594920269 creator A5079070105 @default.
• W1594920269 creator A5085709802 @default.
• W1594920269 date "1997-04-15" @default.
• W1594920269 modified "2023-10-18" @default.
• W1594920269 title "Biologic properties of a bispecific single-chain antibody directed against 17-1A (EpCAM) and CD3: tumor cell-dependent T cell stimulation and cytotoxic activity." @default.
• W1594920269 cites W1493845674 @default.
• W1594920269 cites W1496938524 @default.
• W1594920269 cites W1563434511 @default.
• W1594920269 cites W1565218273 @default.
• W1594920269 cites W1572912927 @default.
• W1594920269 cites W1634491459 @default.
• W1594920269 cites W1898562538 @default.
• W1594920269 cites W1972201132 @default.
• W1594920269 cites W1976722345 @default.
• W1594920269 cites W1981218638 @default.
• W1594920269 cites W1983267929 @default.
• W1594920269 cites W2065272367 @default.
• W1594920269 cites W2072281294 @default.
• W1594920269 cites W2073914550 @default.
• W1594920269 cites W2074619593 @default.
• W1594920269 cites W2110067379 @default.
• W1594920269 cites W2116136455 @default.
• W1594920269 cites W2127993518 @default.
• W1594920269 cites W2331827162 @default.
• W1594920269 doi "https://doi.org/10.4049/jimmunol.158.8.3965" @default.
• W1594920269 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9103467" @default.
• W1594920269 hasPublicationYear "1997" @default.
• W1594920269 type Work @default.
• W1594920269 sameAs 1594920269 @default.
• W1594920269 citedByCount "60" @default.
• W1594920269 countsByYear W15949202692012 @default.
• W1594920269 countsByYear W15949202692013 @default.
• W1594920269 countsByYear W15949202692014 @default.
• W1594920269 countsByYear W15949202692015 @default.
• W1594920269 countsByYear W15949202692016 @default.
• W1594920269 countsByYear W15949202692017 @default.
• W1594920269 countsByYear W15949202692018 @default.
• W1594920269 countsByYear W15949202692019 @default.
• W1594920269 countsByYear W15949202692022 @default.
• W1594920269 crossrefType "journal-article" @default.
• W1594920269 hasAuthorship W1594920269A5001528551 @default.
• W1594920269 hasAuthorship W1594920269A5008627097 @default.
• W1594920269 hasAuthorship W1594920269A5009261802 @default.
• W1594920269 hasAuthorship W1594920269A5079070105 @default.
• W1594920269 hasAuthorship W1594920269A5085709802 @default.
• W1594920269 hasBestOaLocation W15949202691 @default.
• W1594920269 hasConcept C109316439 @default.
• W1594920269 hasConcept C114684123 @default.
• W1594920269 hasConcept C147483822 @default.
• W1594920269 hasConcept C148125776 @default.
• W1594920269 hasConcept C153911025 @default.
• W1594920269 hasConcept C154317977 @default.
• W1594920269 hasConcept C159654299 @default.
• W1594920269 hasConcept C167672396 @default.
• W1594920269 hasConcept C170627219 @default.
• W1594920269 hasConcept C175656101 @default.
• W1594920269 hasConcept C185592680 @default.
• W1594920269 hasConcept C202751555 @default.
• W1594920269 hasConcept C203014093 @default.
• W1594920269 hasConcept C2776090121 @default.
• W1594920269 hasConcept C502942594 @default.
• W1594920269 hasConcept C54355233 @default.
• W1594920269 hasConcept C55493867 @default.
• W1594920269 hasConcept C81885089 @default.
• W1594920269 hasConcept C86803240 @default.
• W1594920269 hasConcept C8891405 @default.
• W1594920269 hasConceptScore W1594920269C109316439 @default.
• W1594920269 hasConceptScore W1594920269C114684123 @default.
• W1594920269 hasConceptScore W1594920269C147483822 @default.
• W1594920269 hasConceptScore W1594920269C148125776 @default.
• W1594920269 hasConceptScore W1594920269C153911025 @default.
• W1594920269 hasConceptScore W1594920269C154317977 @default.
• W1594920269 hasConceptScore W1594920269C159654299 @default.
• W1594920269 hasConceptScore W1594920269C167672396 @default.
• W1594920269 hasConceptScore W1594920269C170627219 @default.
• W1594920269 hasConceptScore W1594920269C175656101 @default.
• W1594920269 hasConceptScore W1594920269C185592680 @default.
• W1594920269 hasConceptScore W1594920269C202751555 @default.
• W1594920269 hasConceptScore W1594920269C203014093 @default.
• W1594920269 hasConceptScore W1594920269C2776090121 @default.
• W1594920269 hasConceptScore W1594920269C502942594 @default.
• W1594920269 hasConceptScore W1594920269C54355233 @default.
• W1594920269 hasConceptScore W1594920269C55493867 @default.
• W1594920269 hasConceptScore W1594920269C81885089 @default.
• W1594920269 hasConceptScore W1594920269C86803240 @default.
• W1594920269 hasConceptScore W1594920269C8891405 @default.
• W1594920269 hasIssue "8" @default.
• W1594920269 hasLocation W15949202691 @default.
• W1594920269 hasLocation W15949202692 @default.
• W1594920269 hasOpenAccess W1594920269 @default.
• W1594920269 hasPrimaryLocation W15949202691 @default.
• W1594920269 hasRelatedWork W2070829609 @default.
• W1594920269 hasRelatedWork W2104714636 @default.

• next