Matches in SemOpenAlex for { <https://semopenalex.org/work/W159536703> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W159536703 endingPage "133.43" @default.
- W159536703 startingPage "133.43" @default.
- W159536703 abstract "Abstract Evidence is building for the role of mast cells (MC) in innate immune responses against various pathogens. In the lungs, MC exist close to epithelial cells (EC), where influenza A virus (FluA) primarily replicates. Viruses such as dengue virus and HIV are capable of replicating in MC, but no report of FluA replication in MC currently exists. Our aim was to characterize FluA infection in human MC and examine host factors in MC that might regulate virus replication. Human MC lines (HMC1 and LAD2) and primary MC cultured from human peripheral blood (PBMC) were exposed to FluA (PR/34/8, 20HAU/ml). EC lines (MDCK and Calu-3) were used as comparative cell types. The presence of RNA viral transcripts by RT-PCR followed similar time courses in MC and EC. Supernatants were harvested at various time points after infection. Total FluA parcticles as determined by ability to agglutinate red blood cells was significantly lower (p<0.05) in MC (27 ± 13 HAU/ml) than in EC (480 ± 160 HAU/ml) at 4 days after infection. Infectious FluA particles as determined by ability of supernatants to infect Calu-3 were detected from EC but not MC. Since FluA replication was diminished in MC compared to EC, we explored whether MC express and/or upregulate antiviral genes in response to FluA. We have data that RNA transcripts of antiviral genes, such as nitric oxide synthase 3 and protein kinase R, are increased in MC exposed to FluA. Our evidence suggests that MC have antiviral mechanisms to restrict FluA replication, which may contribute to host immunity." @default.
- W159536703 created "2016-06-24" @default.
- W159536703 creator A5025415432 @default.
- W159536703 creator A5044249724 @default.
- W159536703 creator A5076473025 @default.
- W159536703 creator A5077672448 @default.
- W159536703 date "2009-04-01" @default.
- W159536703 modified "2023-10-16" @default.
- W159536703 title "Influenza A Virus Infects Human Mast Cells and Increases Expression of Antiviral Genes (133.43)" @default.
- W159536703 doi "https://doi.org/10.4049/jimmunol.182.supp.133.43" @default.
- W159536703 hasPublicationYear "2009" @default.
- W159536703 type Work @default.
- W159536703 sameAs 159536703 @default.
- W159536703 citedByCount "1" @default.
- W159536703 crossrefType "journal-article" @default.
- W159536703 hasAuthorship W159536703A5025415432 @default.
- W159536703 hasAuthorship W159536703A5044249724 @default.
- W159536703 hasAuthorship W159536703A5076473025 @default.
- W159536703 hasAuthorship W159536703A5077672448 @default.
- W159536703 hasConcept C104317684 @default.
- W159536703 hasConcept C136449434 @default.
- W159536703 hasConcept C140704245 @default.
- W159536703 hasConcept C153911025 @default.
- W159536703 hasConcept C159047783 @default.
- W159536703 hasConcept C203014093 @default.
- W159536703 hasConcept C2522874641 @default.
- W159536703 hasConcept C54355233 @default.
- W159536703 hasConcept C67705224 @default.
- W159536703 hasConcept C81885089 @default.
- W159536703 hasConcept C86803240 @default.
- W159536703 hasConcept C8891405 @default.
- W159536703 hasConceptScore W159536703C104317684 @default.
- W159536703 hasConceptScore W159536703C136449434 @default.
- W159536703 hasConceptScore W159536703C140704245 @default.
- W159536703 hasConceptScore W159536703C153911025 @default.
- W159536703 hasConceptScore W159536703C159047783 @default.
- W159536703 hasConceptScore W159536703C203014093 @default.
- W159536703 hasConceptScore W159536703C2522874641 @default.
- W159536703 hasConceptScore W159536703C54355233 @default.
- W159536703 hasConceptScore W159536703C67705224 @default.
- W159536703 hasConceptScore W159536703C81885089 @default.
- W159536703 hasConceptScore W159536703C86803240 @default.
- W159536703 hasConceptScore W159536703C8891405 @default.
- W159536703 hasIssue "1_Supplement" @default.
- W159536703 hasLocation W1595367031 @default.
- W159536703 hasOpenAccess W159536703 @default.
- W159536703 hasPrimaryLocation W1595367031 @default.
- W159536703 hasRelatedWork W1608862421 @default.
- W159536703 hasRelatedWork W1825162875 @default.
- W159536703 hasRelatedWork W1990141933 @default.
- W159536703 hasRelatedWork W1991126365 @default.
- W159536703 hasRelatedWork W2010478346 @default.
- W159536703 hasRelatedWork W2021255975 @default.
- W159536703 hasRelatedWork W2086632997 @default.
- W159536703 hasRelatedWork W2142935324 @default.
- W159536703 hasRelatedWork W2406270448 @default.
- W159536703 hasRelatedWork W3165029026 @default.
- W159536703 hasVolume "182" @default.
- W159536703 isParatext "false" @default.
- W159536703 isRetracted "false" @default.
- W159536703 magId "159536703" @default.
- W159536703 workType "article" @default.