Matches in SemOpenAlex for { <https://semopenalex.org/work/W1595546724> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W1595546724 abstract "A101 We have developed a systematic and stepwise genome-scale screening system to discover a new panel of genes enable to represent the high frequency of promoter methylation in the cervical cancer and its precursor lesions including carcinoma in situ (CIS) and high-grade squamous intraepithelial(HSIL). Considering that aberrant promoter methylation-mediated silencing of tumor suppressor genes has significant tissue specificity, we firstly investigated a subset of genes relatively decreased in gene expression in cervical cancer(CC) and biopsy specimens of precursor lesions (CC in 8, CIS in 2, and HSIL in 5) compared with normal-appearing biopsies in 7 using 35K microarray. We secondly analyzed expression profiles to identify genes epigenetically reactivated in four CC derived cell lines after treatment with methylation inhibiting agent, 5-aza-29-deoxycytidine. We obtained 53 genes by cross-comparison two gene lists and reduced to 28 through two additional steps, in silico search for whether CpG islands exist or not and following examination for methylation status of each gene by PCR after digestion with restriction-enzymes, Hpa II and Msp I, in 4 CC cell lines. To determine the degree of methylation frequency of 28 filtered genes in CC or its precursor cells present at exfoliated cell samples, each gene was examined with DNA of smears from patients with CC in 1, CIS in 2, HSIL in 3, LSIL in 3, and normal in 3. We selected a panel of 10 genes for further clinical validations because they showed the high frequency of methylation in HSIL or worse while very low in LSIL or less. We then evaluated the panel as a CpG islands methylator phenotype (CIMP) with 134 cytological smears diagnosed as normal in 70, atypical squamous cell of undetermined significance (ASCUS) in 5, LSIL in 26, HSIL in 11, CIS in 10 and CC in 12. The frequency of methylation was overall increased with increasing grade of histological abnormality. Five out of ten showed a statistical significance ( p" @default.
- W1595546724 created "2016-06-24" @default.
- W1595546724 creator A5001984145 @default.
- W1595546724 creator A5010364211 @default.
- W1595546724 creator A5011196160 @default.
- W1595546724 creator A5012586082 @default.
- W1595546724 creator A5023615914 @default.
- W1595546724 creator A5042586509 @default.
- W1595546724 creator A5060265528 @default.
- W1595546724 creator A5073089636 @default.
- W1595546724 creator A5077891405 @default.
- W1595546724 creator A5081032878 @default.
- W1595546724 creator A5090832721 @default.
- W1595546724 date "2006-10-01" @default.
- W1595546724 modified "2023-09-27" @default.
- W1595546724 title "Translation gene expression profiling into the identification of high frequency of methylation markers in cervical neoplasia: novel panel of 3 genes of the CpG island methylator phenotype(CIMP) for high-grade squamous intraepithelial (HSIL) or worse." @default.
- W1595546724 hasPublicationYear "2006" @default.
- W1595546724 type Work @default.
- W1595546724 sameAs 1595546724 @default.
- W1595546724 citedByCount "0" @default.
- W1595546724 crossrefType "journal-article" @default.
- W1595546724 hasAuthorship W1595546724A5001984145 @default.
- W1595546724 hasAuthorship W1595546724A5010364211 @default.
- W1595546724 hasAuthorship W1595546724A5011196160 @default.
- W1595546724 hasAuthorship W1595546724A5012586082 @default.
- W1595546724 hasAuthorship W1595546724A5023615914 @default.
- W1595546724 hasAuthorship W1595546724A5042586509 @default.
- W1595546724 hasAuthorship W1595546724A5060265528 @default.
- W1595546724 hasAuthorship W1595546724A5073089636 @default.
- W1595546724 hasAuthorship W1595546724A5077891405 @default.
- W1595546724 hasAuthorship W1595546724A5081032878 @default.
- W1595546724 hasAuthorship W1595546724A5090832721 @default.
- W1595546724 hasConcept C104317684 @default.
- W1595546724 hasConcept C140173407 @default.
- W1595546724 hasConcept C150194340 @default.
- W1595546724 hasConcept C153911025 @default.
- W1595546724 hasConcept C190727270 @default.
- W1595546724 hasConcept C33288867 @default.
- W1595546724 hasConcept C502942594 @default.
- W1595546724 hasConcept C54355233 @default.
- W1595546724 hasConcept C86803240 @default.
- W1595546724 hasConceptScore W1595546724C104317684 @default.
- W1595546724 hasConceptScore W1595546724C140173407 @default.
- W1595546724 hasConceptScore W1595546724C150194340 @default.
- W1595546724 hasConceptScore W1595546724C153911025 @default.
- W1595546724 hasConceptScore W1595546724C190727270 @default.
- W1595546724 hasConceptScore W1595546724C33288867 @default.
- W1595546724 hasConceptScore W1595546724C502942594 @default.
- W1595546724 hasConceptScore W1595546724C54355233 @default.
- W1595546724 hasConceptScore W1595546724C86803240 @default.
- W1595546724 hasLocation W15955467241 @default.
- W1595546724 hasOpenAccess W1595546724 @default.
- W1595546724 hasPrimaryLocation W15955467241 @default.
- W1595546724 hasRelatedWork W1964488253 @default.
- W1595546724 hasRelatedWork W1971599064 @default.
- W1595546724 hasRelatedWork W1984409247 @default.
- W1595546724 hasRelatedWork W1999150369 @default.
- W1595546724 hasRelatedWork W1999380552 @default.
- W1595546724 hasRelatedWork W2028432928 @default.
- W1595546724 hasRelatedWork W2049169460 @default.
- W1595546724 hasRelatedWork W2065595129 @default.
- W1595546724 hasRelatedWork W2076196625 @default.
- W1595546724 hasRelatedWork W2083319219 @default.
- W1595546724 hasRelatedWork W2145817847 @default.
- W1595546724 hasRelatedWork W2597703148 @default.
- W1595546724 hasRelatedWork W2601985623 @default.
- W1595546724 hasRelatedWork W2793017752 @default.
- W1595546724 hasRelatedWork W2894310214 @default.
- W1595546724 hasRelatedWork W2904786626 @default.
- W1595546724 hasRelatedWork W2986684914 @default.
- W1595546724 hasRelatedWork W3025527339 @default.
- W1595546724 hasRelatedWork W3046474844 @default.
- W1595546724 hasRelatedWork W3148492544 @default.
- W1595546724 hasVolume "12" @default.
- W1595546724 isParatext "false" @default.
- W1595546724 isRetracted "false" @default.
- W1595546724 magId "1595546724" @default.
- W1595546724 workType "article" @default.