Matches in SemOpenAlex for { <https://semopenalex.org/work/W1595640523> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1595640523 endingPage "71" @default.
- W1595640523 startingPage "67" @default.
- W1595640523 abstract "Radioactivity from S-adenosyl-l-[methyl-3H]methionine ([methyl-3H]AdoMet) was bound to the EcoP15 DNA methyltransferase (M · EcoP15) following short-wave ultraviolet (UV) irradiation. The labeled protein was subjected to polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE), and detected by fluorography and autoradiography. Labeling was found to be dependent on the concentration of AdoMet and time of UV irradiation. The photolabeling by [methyl3H]AdoMet was specific and blocked by S-adenosyl-l-homocysteine (AdoHcy) and sinefungin which are known to function as competitive inhibitors. Limited digestion of the M · EcoP15-AdoMet adduct by Staphylococcus aureus protease V8 generated three peptides of approx. 50, 32 and 30 kDa. Interestingly, only the 30-kDa peptide fragment contained radioactivity, as detected by SDS-PAGE, followed by fluorography and autoradiog- raphy. Further, sequencing of a few amino acids at the N-terminus of these peptides showed that the 30-kDa fragment was the N-terminal portion of M · EcoP15. These results suggest that photolabeling is at the AdoMet-binding site and that the N-terminal half of M · EcoP15 may be involved in substrate binding." @default.
- W1595640523 created "2016-06-24" @default.
- W1595640523 creator A5021599987 @default.
- W1595640523 creator A5041421289 @default.
- W1595640523 date "1994-05-01" @default.
- W1595640523 modified "2023-09-25" @default.
- W1595640523 title "Photolabeling of the EcoP15 DNA methyltransferase with" @default.
- W1595640523 cites W1492313802 @default.
- W1595640523 cites W1511477775 @default.
- W1595640523 cites W1524018512 @default.
- W1595640523 cites W1550216293 @default.
- W1595640523 cites W1679042648 @default.
- W1595640523 cites W1964418545 @default.
- W1595640523 cites W1976720990 @default.
- W1595640523 cites W2004989533 @default.
- W1595640523 cites W2030305640 @default.
- W1595640523 cites W2046362520 @default.
- W1595640523 cites W2064088137 @default.
- W1595640523 cites W2076277117 @default.
- W1595640523 cites W2100837269 @default.
- W1595640523 cites W2167611648 @default.
- W1595640523 cites W2179631201 @default.
- W1595640523 doi "https://doi.org/10.1016/0378-1119(94)90356-5" @default.
- W1595640523 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8181759" @default.
- W1595640523 hasPublicationYear "1994" @default.
- W1595640523 type Work @default.
- W1595640523 sameAs 1595640523 @default.
- W1595640523 citedByCount "8" @default.
- W1595640523 crossrefType "journal-article" @default.
- W1595640523 hasAuthorship W1595640523A5021599987 @default.
- W1595640523 hasAuthorship W1595640523A5041421289 @default.
- W1595640523 hasConcept C101660505 @default.
- W1595640523 hasConcept C116084860 @default.
- W1595640523 hasConcept C153911025 @default.
- W1595640523 hasConcept C181199279 @default.
- W1595640523 hasConcept C185592680 @default.
- W1595640523 hasConcept C2776714187 @default.
- W1595640523 hasConcept C2778913249 @default.
- W1595640523 hasConcept C2779281246 @default.
- W1595640523 hasConcept C2780912031 @default.
- W1595640523 hasConcept C2987116853 @default.
- W1595640523 hasConcept C33288867 @default.
- W1595640523 hasConcept C515207424 @default.
- W1595640523 hasConcept C552990157 @default.
- W1595640523 hasConcept C55493867 @default.
- W1595640523 hasConcept C75473681 @default.
- W1595640523 hasConcept C86803240 @default.
- W1595640523 hasConcept C91965660 @default.
- W1595640523 hasConceptScore W1595640523C101660505 @default.
- W1595640523 hasConceptScore W1595640523C116084860 @default.
- W1595640523 hasConceptScore W1595640523C153911025 @default.
- W1595640523 hasConceptScore W1595640523C181199279 @default.
- W1595640523 hasConceptScore W1595640523C185592680 @default.
- W1595640523 hasConceptScore W1595640523C2776714187 @default.
- W1595640523 hasConceptScore W1595640523C2778913249 @default.
- W1595640523 hasConceptScore W1595640523C2779281246 @default.
- W1595640523 hasConceptScore W1595640523C2780912031 @default.
- W1595640523 hasConceptScore W1595640523C2987116853 @default.
- W1595640523 hasConceptScore W1595640523C33288867 @default.
- W1595640523 hasConceptScore W1595640523C515207424 @default.
- W1595640523 hasConceptScore W1595640523C552990157 @default.
- W1595640523 hasConceptScore W1595640523C55493867 @default.
- W1595640523 hasConceptScore W1595640523C75473681 @default.
- W1595640523 hasConceptScore W1595640523C86803240 @default.
- W1595640523 hasConceptScore W1595640523C91965660 @default.
- W1595640523 hasIssue "1" @default.
- W1595640523 hasLocation W15956405231 @default.
- W1595640523 hasLocation W15956405232 @default.
- W1595640523 hasOpenAccess W1595640523 @default.
- W1595640523 hasPrimaryLocation W15956405231 @default.
- W1595640523 hasRelatedWork W1440031586 @default.
- W1595640523 hasRelatedWork W1489696392 @default.
- W1595640523 hasRelatedWork W2005770668 @default.
- W1595640523 hasRelatedWork W2032398980 @default.
- W1595640523 hasRelatedWork W2034205185 @default.
- W1595640523 hasRelatedWork W2037536077 @default.
- W1595640523 hasRelatedWork W2074738418 @default.
- W1595640523 hasRelatedWork W2085491026 @default.
- W1595640523 hasRelatedWork W2124813013 @default.
- W1595640523 hasRelatedWork W2463802760 @default.
- W1595640523 hasVolume "142" @default.
- W1595640523 isParatext "false" @default.
- W1595640523 isRetracted "false" @default.
- W1595640523 magId "1595640523" @default.
- W1595640523 workType "article" @default.