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- W1595701475 abstract "Abstract Isolated cell walls of Bacillus licheniformis ATCC 9945 were digested with lysozyme, and a low molecular weight fraction was obtained after dialysis of the digest. A disaccharide and two disaccharide peptides were isolated from this fraction by gel filtration on a Bio-Gel P-4 column and chromatography on Dowex 50. The structure of the isolated compounds was studied by chemical and enzymatic techniques. The disaccharide was found to be identical with O-2-acetamido-2-deoxy-β-d-glucopyranosyl-(1→4)-2-acetamido-3-O-(d-1-carboxyethyl)-2-deoxy-d-glucose (GlcNAcβ-(1 → 4)MurNAc) previously isolated from Micrococcus lysodeikticus cell walls. The two disaccharide peptides were shown to be tripeptide derivatives of this disaccharide, wherein the peptide is linked to the disaccharide by an amide bond between the carboxyl group of the muramic acid of the latter and the NH2-terminal amino group of the peptide. One of the disaccharide peptides was shown to have the structure [see PDF for structure] (shown in Fig. 4). The other has the same structure as this, but in addition contains an amide group on the α-COOH of glutamic acid. Small amounts of a tetrasaccharide, GlcNAcβ-(1 → 4)MurNAcβ(1 → 4)GlcNacβ(1 → 4)MurNAc, were also isolated." @default.
- W1595701475 created "2016-06-24" @default.
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- W1595701475 date "1968-05-01" @default.
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- W1595701475 title "Isolation and Characterization of the Disaccharide N-Acetylglucosaminyl-β(1→4)-N-acetylmuramic Acid and Two Tripeptide Derivatives of This Disaccharide from Lysozyme Digests of Bacillus licheniformis ATCC 9945 Cell Walls" @default.
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- W1595701475 doi "https://doi.org/10.1016/s0021-9258(18)93473-3" @default.
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