FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1595781572> ?p ?o ?g. }

• W1595781572 endingPage "6080" @default.
• W1595781572 startingPage "6071" @default.
• W1595781572 abstract "The yeast Saccharomyces cerevisiae expresses two phosphatidylserine decarboxylase (PSD) activities which are responsible for conversion of phosphatidylserine to phosphatidylethanolamine, and either enzyme alone is sufficient for normal cellular growth. However, strains containing a PSD1 null allele and a mutation leading to loss of PSD2 activity (psd1-Δ1::TRP1 psd2) are auxotrophic for ethanolamine. This nutritional requirement was utilized to isolate the gene encoding the PSD2 enzyme by complementation. The PSD2 gene encodes a protein of 1138 amino acids with a predicted molecular mass of 130 kDa. The deduced amino acid sequence shows significant identity (34%) to a PSD-like sequence from Clostridium pasteurianum and the yeast PSD1 (19%) at the carboxyl end of the protein. Of particular interest is the presence of a sequence, GGST, which may be involved in post-translational processing and prosthetic group formation similar to other PSD enzymes. The PSD2 amino acid sequence also shows significant homology to the C2 regions of protein kinase C and synaptotagmin. Physical mapping experiments demonstrate that the PSD2 is located on chromosome 7. The PSD2 gene was heterologously expressed by infection of Sf-9 insect cells with recombinant baculovirus, resulting in a 10-fold increase in PSD activity. The null allele of PSD2 was introduced into yeast strains by one-step gene deletion/disruption with a HIS3 marker gene. Strains expressing wild type PSD1 and the psd2-Δ1::HIS3 allele show a small decrease in overall PSD activity, but no noticeable effect upon [3H]serine incorporation into aminophospholipids. Strains containing both the psd1-Δ1::TRP1 and psd2-Δ1::HIS3 null alleles, however, express no detectable PSD activity, are ethanolamine auxotrophs and show a severe deficit in the conversion of [3H]serine-labeled phosphatidylserine to phosphatidylethanolamine. These data indicate that the gene isolated is the structural gene for PSD2 and that the PSD1 and PSD2 enzymes account for all yeast PSD activity. The yeast Saccharomyces cerevisiae expresses two phosphatidylserine decarboxylase (PSD) activities which are responsible for conversion of phosphatidylserine to phosphatidylethanolamine, and either enzyme alone is sufficient for normal cellular growth. However, strains containing a PSD1 null allele and a mutation leading to loss of PSD2 activity (psd1-Δ1::TRP1 psd2) are auxotrophic for ethanolamine. This nutritional requirement was utilized to isolate the gene encoding the PSD2 enzyme by complementation. The PSD2 gene encodes a protein of 1138 amino acids with a predicted molecular mass of 130 kDa. The deduced amino acid sequence shows significant identity (34%) to a PSD-like sequence from Clostridium pasteurianum and the yeast PSD1 (19%) at the carboxyl end of the protein. Of particular interest is the presence of a sequence, GGST, which may be involved in post-translational processing and prosthetic group formation similar to other PSD enzymes. The PSD2 amino acid sequence also shows significant homology to the C2 regions of protein kinase C and synaptotagmin. Physical mapping experiments demonstrate that the PSD2 is located on chromosome 7. The PSD2 gene was heterologously expressed by infection of Sf-9 insect cells with recombinant baculovirus, resulting in a 10-fold increase in PSD activity. The null allele of PSD2 was introduced into yeast strains by one-step gene deletion/disruption with a HIS3 marker gene. Strains expressing wild type PSD1 and the psd2-Δ1::HIS3 allele show a small decrease in overall PSD activity, but no noticeable effect upon [3H]serine incorporation into aminophospholipids. Strains containing both the psd1-Δ1::TRP1 and psd2-Δ1::HIS3 null alleles, however, express no detectable PSD activity, are ethanolamine auxotrophs and show a severe deficit in the conversion of [3H]serine-labeled phosphatidylserine to phosphatidylethanolamine. These data indicate that the gene isolated is the structural gene for PSD2 and that the PSD1 and PSD2 enzymes account for all yeast PSD activity." @default.
• W1595781572 created "2016-06-24" @default.
• W1595781572 creator A5029911293 @default.
• W1595781572 creator A5044700206 @default.
• W1595781572 creator A5069382327 @default.
• W1595781572 creator A5088383763 @default.
• W1595781572 date "1995-03-01" @default.
• W1595781572 modified "2023-10-14" @default.
• W1595781572 title "Phosphatidylserine Decarboxylase 2 of Saccharomyces cerevisiáe" @default.
• W1595781572 cites W1016749220 @default.
• W1595781572 cites W1481171708 @default.
• W1595781572 cites W1517480469 @default.
• W1595781572 cites W1539603883 @default.
• W1595781572 cites W1545339883 @default.
• W1595781572 cites W1545684494 @default.
• W1595781572 cites W1547269929 @default.
• W1595781572 cites W1568294354 @default.
• W1595781572 cites W1568352573 @default.
• W1595781572 cites W1575864823 @default.
• W1595781572 cites W1579180733 @default.
• W1595781572 cites W1588347494 @default.
• W1595781572 cites W1591133722 @default.
• W1595781572 cites W1597834912 @default.
• W1595781572 cites W1653089865 @default.
• W1595781572 cites W1845128137 @default.
• W1595781572 cites W1927096570 @default.
• W1595781572 cites W1968162244 @default.
• W1595781572 cites W1978406634 @default.
• W1595781572 cites W1984764741 @default.
• W1595781572 cites W1985001103 @default.
• W1595781572 cites W1989231246 @default.
• W1595781572 cites W2002652302 @default.
• W1595781572 cites W2016459483 @default.
• W1595781572 cites W2025624617 @default.
• W1595781572 cites W2025906428 @default.
• W1595781572 cites W2054800842 @default.
• W1595781572 cites W2055043387 @default.
• W1595781572 cites W2069624943 @default.
• W1595781572 cites W2072949813 @default.
• W1595781572 cites W2072964604 @default.
• W1595781572 cites W2074030569 @default.
• W1595781572 cites W2087726910 @default.
• W1595781572 cites W2098329073 @default.
• W1595781572 cites W2111342014 @default.
• W1595781572 cites W2137433289 @default.
• W1595781572 cites W2138270253 @default.
• W1595781572 cites W2268036223 @default.
• W1595781572 cites W2342453669 @default.
• W1595781572 cites W2418815503 @default.
• W1595781572 cites W2528198080 @default.
• W1595781572 cites W2753765968 @default.
• W1595781572 cites W2770215539 @default.
• W1595781572 cites W4240065971 @default.
• W1595781572 cites W955649814 @default.
• W1595781572 cites W96549249 @default.
• W1595781572 doi "https://doi.org/10.1074/jbc.270.11.6071" @default.
• W1595781572 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7890740" @default.
• W1595781572 hasPublicationYear "1995" @default.
• W1595781572 type Work @default.
• W1595781572 sameAs 1595781572 @default.
• W1595781572 citedByCount "162" @default.
• W1595781572 countsByYear W15957815722012 @default.
• W1595781572 countsByYear W15957815722013 @default.
• W1595781572 countsByYear W15957815722014 @default.
• W1595781572 countsByYear W15957815722015 @default.
• W1595781572 countsByYear W15957815722016 @default.
• W1595781572 countsByYear W15957815722017 @default.
• W1595781572 countsByYear W15957815722018 @default.
• W1595781572 countsByYear W15957815722019 @default.
• W1595781572 countsByYear W15957815722020 @default.
• W1595781572 countsByYear W15957815722021 @default.
• W1595781572 countsByYear W15957815722022 @default.
• W1595781572 countsByYear W15957815722023 @default.
• W1595781572 crossrefType "journal-article" @default.
• W1595781572 hasAuthorship W1595781572A5029911293 @default.
• W1595781572 hasAuthorship W1595781572A5044700206 @default.
• W1595781572 hasAuthorship W1595781572A5069382327 @default.
• W1595781572 hasAuthorship W1595781572A5088383763 @default.
• W1595781572 hasBestOaLocation W15957815721 @default.
• W1595781572 hasConcept C104317684 @default.
• W1595781572 hasConcept C143065580 @default.
• W1595781572 hasConcept C153911025 @default.
• W1595781572 hasConcept C181199279 @default.
• W1595781572 hasConcept C188082640 @default.
• W1595781572 hasConcept C20950039 @default.
• W1595781572 hasConcept C2776330855 @default.
• W1595781572 hasConcept C2776414213 @default.
• W1595781572 hasConcept C2777576037 @default.
• W1595781572 hasConcept C2778918659 @default.
• W1595781572 hasConcept C2779637612 @default.
• W1595781572 hasConcept C2781170229 @default.
• W1595781572 hasConcept C41625074 @default.
• W1595781572 hasConcept C547475151 @default.
• W1595781572 hasConcept C55493867 @default.
• W1595781572 hasConcept C86803240 @default.
• W1595781572 hasConceptScore W1595781572C104317684 @default.
• W1595781572 hasConceptScore W1595781572C143065580 @default.
• W1595781572 hasConceptScore W1595781572C153911025 @default.

• next