Matches in SemOpenAlex for { <https://semopenalex.org/work/W1596163449> ?p ?o ?g. }
- W1596163449 endingPage "21853" @default.
- W1596163449 startingPage "21844" @default.
- W1596163449 abstract "Abstract The 119-kDa primary translation product of the VMA1 gene of Saccharomyces cerevisiae undergoes a self-catalyzed rearrangement (protein splicing) that excises an internal 50-kDa segment of the polypeptide and joins the amino-terminal and carboxyl-terminal segments to generate the 69-kDa subunit of the vacuolar membrane-associated H(+)-ATPase. We have shown previously that the internal segment is a site-specific endonuclease (Gimble, F. S., and Thorner, J. (1992) Nature 357, 301-306). Here we describe methods for the high level expression and purification to near homogeneity of both the authentic VMA1-derived endonuclease (or VDE) from yeast (yield 18%) and a recombinant form of VDE made in bacteria (yield 29%). Detailed characterization of these preparations demonstrated that the yeast-derived and bacterially produced enzymes were indistinguishable, as judged by: (a) behavior during purification; (b) apparent native molecular mass (50 kDa); (c) immunological reactivity; and (d) catalytic properties (specific activity; cleavage site recognition; and optima for pH, temperature, divalent cation and ionic strength). The minimal site required for VDE cleavage was delimited to a 30-base pair sequence within its specific substrate (the VMA1 delta vde allele)." @default.
- W1596163449 created "2016-06-24" @default.
- W1596163449 creator A5048920959 @default.
- W1596163449 creator A5056113292 @default.
- W1596163449 date "1993-10-01" @default.
- W1596163449 modified "2023-09-29" @default.
- W1596163449 title "Purification and characterization of VDE, a site-specific endonuclease from the yeast Saccharomyces cerevisiae." @default.
- W1596163449 cites W1481254609 @default.
- W1596163449 cites W1579306860 @default.
- W1596163449 cites W1592537807 @default.
- W1596163449 cites W1595683668 @default.
- W1596163449 cites W160422442 @default.
- W1596163449 cites W1873199501 @default.
- W1596163449 cites W1966504749 @default.
- W1596163449 cites W1966578724 @default.
- W1596163449 cites W1973335730 @default.
- W1596163449 cites W1973782344 @default.
- W1596163449 cites W1975987535 @default.
- W1596163449 cites W1977120851 @default.
- W1596163449 cites W1991213785 @default.
- W1596163449 cites W1998348841 @default.
- W1596163449 cites W2002502414 @default.
- W1596163449 cites W2011795802 @default.
- W1596163449 cites W2013731604 @default.
- W1596163449 cites W2018050027 @default.
- W1596163449 cites W2022971724 @default.
- W1596163449 cites W2027875921 @default.
- W1596163449 cites W2030628776 @default.
- W1596163449 cites W2040967876 @default.
- W1596163449 cites W2045728515 @default.
- W1596163449 cites W2046702756 @default.
- W1596163449 cites W2047049388 @default.
- W1596163449 cites W2048052068 @default.
- W1596163449 cites W2048052722 @default.
- W1596163449 cites W2053233602 @default.
- W1596163449 cites W2054823031 @default.
- W1596163449 cites W2055703133 @default.
- W1596163449 cites W2059190205 @default.
- W1596163449 cites W2062071185 @default.
- W1596163449 cites W2068183729 @default.
- W1596163449 cites W2068518785 @default.
- W1596163449 cites W2068825288 @default.
- W1596163449 cites W2070596390 @default.
- W1596163449 cites W2074007751 @default.
- W1596163449 cites W2079837880 @default.
- W1596163449 cites W2081585453 @default.
- W1596163449 cites W2081756307 @default.
- W1596163449 cites W2086224954 @default.
- W1596163449 cites W2092171870 @default.
- W1596163449 cites W2092202372 @default.
- W1596163449 cites W2116947837 @default.
- W1596163449 cites W2122078632 @default.
- W1596163449 cites W2122488874 @default.
- W1596163449 cites W2138270253 @default.
- W1596163449 cites W2152331729 @default.
- W1596163449 cites W2169333620 @default.
- W1596163449 cites W2175910164 @default.
- W1596163449 cites W263820944 @default.
- W1596163449 cites W4293247451 @default.
- W1596163449 doi "https://doi.org/10.1016/s0021-9258(20)80618-8" @default.
- W1596163449 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8408039" @default.
- W1596163449 hasPublicationYear "1993" @default.
- W1596163449 type Work @default.
- W1596163449 sameAs 1596163449 @default.
- W1596163449 citedByCount "62" @default.
- W1596163449 countsByYear W15961634492016 @default.
- W1596163449 countsByYear W15961634492017 @default.
- W1596163449 countsByYear W15961634492018 @default.
- W1596163449 countsByYear W15961634492019 @default.
- W1596163449 countsByYear W15961634492020 @default.
- W1596163449 crossrefType "journal-article" @default.
- W1596163449 hasAuthorship W1596163449A5048920959 @default.
- W1596163449 hasAuthorship W1596163449A5056113292 @default.
- W1596163449 hasBestOaLocation W15961634491 @default.
- W1596163449 hasConcept C181199279 @default.
- W1596163449 hasConcept C185592680 @default.
- W1596163449 hasConcept C2777028655 @default.
- W1596163449 hasConcept C2777576037 @default.
- W1596163449 hasConcept C2779222958 @default.
- W1596163449 hasConcept C2992884146 @default.
- W1596163449 hasConcept C55493867 @default.
- W1596163449 hasConcept C86803240 @default.
- W1596163449 hasConceptScore W1596163449C181199279 @default.
- W1596163449 hasConceptScore W1596163449C185592680 @default.
- W1596163449 hasConceptScore W1596163449C2777028655 @default.
- W1596163449 hasConceptScore W1596163449C2777576037 @default.
- W1596163449 hasConceptScore W1596163449C2779222958 @default.
- W1596163449 hasConceptScore W1596163449C2992884146 @default.
- W1596163449 hasConceptScore W1596163449C55493867 @default.
- W1596163449 hasConceptScore W1596163449C86803240 @default.
- W1596163449 hasIssue "29" @default.
- W1596163449 hasLocation W15961634491 @default.
- W1596163449 hasOpenAccess W1596163449 @default.
- W1596163449 hasPrimaryLocation W15961634491 @default.
- W1596163449 hasRelatedWork W1965635048 @default.
- W1596163449 hasRelatedWork W2001703407 @default.
- W1596163449 hasRelatedWork W2021327083 @default.
- W1596163449 hasRelatedWork W2059086631 @default.