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- W1596343071 abstract "Normal replication of Moloney murine leukemia virus (MoMLV) requires the integration of a DNA copy of the viral RNA genome into a chromosome of the host. In this work, we characterize the DNA sequences at the ends of the linear proviral precursor that are required for integration in the presence of MoMLV integration protein in vitro. We found that nine bases of MoMLV DNA at each end of a linear model substrate were sufficient for near-maximal levels of integration and that four bases of MoMLV DNA at each end were sufficient for low levels of correct integration. We also found that a 3'-terminal A residue was preferred for integration. We infer from the limited DNA sequence requirements for integration that factors in addition to DNA sequence direct integration protein to act at the ends of the viral DNA." @default.
- W1596343071 created "2016-06-24" @default.
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- W1596343071 date "1990-11-01" @default.
- W1596343071 modified "2023-10-14" @default.
- W1596343071 title "Sequence requirements for integration of Moloney murine leukemia virus DNA in vitro" @default.
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- W1596343071 doi "https://doi.org/10.1128/jvi.64.11.5645-5648.1990" @default.
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