FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1596365682> ?p ?o ?g. }

• W1596365682 endingPage "1124" @default.
• W1596365682 startingPage "1113" @default.
• W1596365682 abstract "To facilitate further studies of flavivirus transcription, cell extraction methods and in vitro reaction conditions which increased West Nile virus (WNV) RNA-dependent RNA polymerase activity were determined. Subcellular fractions from WNV-infected BHK-21/W12 cells were characterized with regard to their protein and RNA content and in vitro polymerase activity. In both a cytoplasmic fraction, designated S1, and a fraction enriched for outer nuclear membranes, designated S2, seven virus-specific proteins, NS5 (96 kilodaltons [kDa]), NS3 (67 kDa), E (48 kDa), NS1 (47 kDa), ns4a (26 kDa), ns2a (17 kDa), and ns2b (14.5 kDa), were detected. The fractions also contained virus-specific RNA and cellular rRNA and mRNA. Polymerase activity in S1 and S2 fractions from WNV-infected cells was concentrated by pelleting and consisted of two types of enzyme activities: the WNV RNA-dependent RNA polymerase and terminal transferases of cellular origin. Enhanced levels of WNV polymerase activity were obtained from these cell fractions by altering several of the in vitro reaction conditions. Although Mg2+ was the divalent cation preferred by WNV polymerase, virus-specific in vitro transcription was detected at reduced levels when Mn2+ (0.05 or 0.5 mM) was present as the sole divalent cation. Product analysis revealed that the viral polymerase incorporated radiolabeled ribonucleotides into three distinct RNA species. Free single-stranded genome-sized RNA which was LiCl insoluble and RNase sensitive was found by fingerprint analysis to have an oligonucleotide pattern similar to that of WNV genomic RNA. RNA molecules which comigrated as a broad band near the top of the gel were separable into LiCl-insoluble, partially RNase-sensitive replicative-intermediate RNA and LiCl-soluble, RNase-resistant replicative-form RNA. The cellular transferases added UMP or AMP residues to the 3'-termini of cellular mRNA, tRNA, and 18S and 28S rRNA. Although a cellular terminal transferase has been reported to function in initiation of poliovirus transcription, no labeling of the WNV RNA by either of these cellular enzymes was detected. Therefore, they appear to play no specific role in flavivirus RNA synthesis." @default.
• W1596365682 created "2016-06-24" @default.
• W1596365682 creator A5016960018 @default.
• W1596365682 creator A5083641020 @default.
• W1596365682 date "1986-12-01" @default.
• W1596365682 modified "2023-10-17" @default.
• W1596365682 title "Characterization of West Nile virus RNA-dependent RNA polymerase and cellular terminal adenylyl and uridylyl transferases in cell-free extracts" @default.
• W1596365682 cites W1508239888 @default.
• W1596365682 cites W1516648546 @default.
• W1596365682 cites W1525535309 @default.
• W1596365682 cites W1538668357 @default.
• W1596365682 cites W1540184643 @default.
• W1596365682 cites W1563029141 @default.
• W1596365682 cites W1902684255 @default.
• W1596365682 cites W1910521101 @default.
• W1596365682 cites W1936300804 @default.
• W1596365682 cites W1954646088 @default.
• W1596365682 cites W1967826883 @default.
• W1596365682 cites W1985120622 @default.
• W1596365682 cites W1988053694 @default.
• W1596365682 cites W1988834597 @default.
• W1596365682 cites W1993382008 @default.
• W1596365682 cites W1994742610 @default.
• W1596365682 cites W2006662253 @default.
• W1596365682 cites W2009671880 @default.
• W1596365682 cites W2015069286 @default.
• W1596365682 cites W2018289835 @default.
• W1596365682 cites W2021083314 @default.
• W1596365682 cites W2027598863 @default.
• W1596365682 cites W2034751478 @default.
• W1596365682 cites W2043974503 @default.
• W1596365682 cites W2064962869 @default.
• W1596365682 cites W2078441700 @default.
• W1596365682 cites W2083854003 @default.
• W1596365682 cites W2089790589 @default.
• W1596365682 cites W2100837269 @default.
• W1596365682 cites W2169900003 @default.
• W1596365682 cites W2230298622 @default.
• W1596365682 cites W2320356625 @default.
• W1596365682 cites W2409436496 @default.
• W1596365682 cites W4245478173 @default.
• W1596365682 doi "https://doi.org/10.1128/jvi.60.3.1113-1124.1986" @default.
• W1596365682 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/253359" @default.
• W1596365682 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3023663" @default.
• W1596365682 hasPublicationYear "1986" @default.
• W1596365682 type Work @default.
• W1596365682 sameAs 1596365682 @default.
• W1596365682 citedByCount "64" @default.
• W1596365682 countsByYear W15963656822013 @default.
• W1596365682 countsByYear W15963656822014 @default.
• W1596365682 countsByYear W15963656822015 @default.
• W1596365682 countsByYear W15963656822017 @default.
• W1596365682 countsByYear W15963656822018 @default.
• W1596365682 countsByYear W15963656822022 @default.
• W1596365682 countsByYear W15963656822023 @default.
• W1596365682 crossrefType "journal-article" @default.
• W1596365682 hasAuthorship W1596365682A5016960018 @default.
• W1596365682 hasAuthorship W1596365682A5083641020 @default.
• W1596365682 hasBestOaLocation W15963656821 @default.
• W1596365682 hasConcept C100299639 @default.
• W1596365682 hasConcept C101762097 @default.
• W1596365682 hasConcept C104317684 @default.
• W1596365682 hasConcept C138885662 @default.
• W1596365682 hasConcept C150194340 @default.
• W1596365682 hasConcept C153911025 @default.
• W1596365682 hasConcept C159047783 @default.
• W1596365682 hasConcept C179926584 @default.
• W1596365682 hasConcept C181199279 @default.
• W1596365682 hasConcept C25281209 @default.
• W1596365682 hasConcept C2776449523 @default.
• W1596365682 hasConcept C41258723 @default.
• W1596365682 hasConcept C41895202 @default.
• W1596365682 hasConcept C55493867 @default.
• W1596365682 hasConcept C64350747 @default.
• W1596365682 hasConcept C67705224 @default.
• W1596365682 hasConcept C82381507 @default.
• W1596365682 hasConcept C86803240 @default.
• W1596365682 hasConceptScore W1596365682C100299639 @default.
• W1596365682 hasConceptScore W1596365682C101762097 @default.
• W1596365682 hasConceptScore W1596365682C104317684 @default.
• W1596365682 hasConceptScore W1596365682C138885662 @default.
• W1596365682 hasConceptScore W1596365682C150194340 @default.
• W1596365682 hasConceptScore W1596365682C153911025 @default.
• W1596365682 hasConceptScore W1596365682C159047783 @default.
• W1596365682 hasConceptScore W1596365682C179926584 @default.
• W1596365682 hasConceptScore W1596365682C181199279 @default.
• W1596365682 hasConceptScore W1596365682C25281209 @default.
• W1596365682 hasConceptScore W1596365682C2776449523 @default.
• W1596365682 hasConceptScore W1596365682C41258723 @default.
• W1596365682 hasConceptScore W1596365682C41895202 @default.
• W1596365682 hasConceptScore W1596365682C55493867 @default.
• W1596365682 hasConceptScore W1596365682C64350747 @default.
• W1596365682 hasConceptScore W1596365682C67705224 @default.
• W1596365682 hasConceptScore W1596365682C82381507 @default.
• W1596365682 hasConceptScore W1596365682C86803240 @default.
• W1596365682 hasIssue "3" @default.
• W1596365682 hasLocation W15963656821 @default.
• W1596365682 hasLocation W15963656822 @default.

• next