Matches in SemOpenAlex for { <https://semopenalex.org/work/W1596411128> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W1596411128 endingPage "9" @default.
- W1596411128 startingPage "192" @default.
- W1596411128 abstract "The transparency and external fertilization of the eggs of medaka (Oryzias latipes) make them ideally suitable for investigating molecular interactions that occur during vertebrate development. Genetically engineered medaka is a potential tool for such studies. It requires several types of suitable expression vectors. To obtain abundant and ubiquitous expression of foreign genes in medaka embryos, we have designed an expression vector that contains the proximal promoter and enhancer elements and polyadenylation signal of the medaka beta-actin gene. The utility of this all-medaka expression vector was examined using the Escherichia coli lacZ gene as a reporter gene. Most of the injected embryo showed high gene expression, and several embryos showed ubiquitous expression even at six days after injection. Of nine individuals derived from the injected embryos and grown until adult stage, one produced expression-positive F1 fish. The transgene was identified in these F1 using polymerase chain reaction (PCR). These data revealed that the expression vector based on the expression cassette from the medaka beta-actin gene should be useful for making transgenic medaka. The cloned gene in this cassette vector is stably transmittable and efficiently expressible." @default.
- W1596411128 created "2016-06-24" @default.
- W1596411128 creator A5029737800 @default.
- W1596411128 creator A5044136355 @default.
- W1596411128 creator A5063467418 @default.
- W1596411128 creator A5065901351 @default.
- W1596411128 creator A5074930965 @default.
- W1596411128 creator A5075718612 @default.
- W1596411128 creator A5086839055 @default.
- W1596411128 date "1994-08-01" @default.
- W1596411128 modified "2023-10-18" @default.
- W1596411128 title "An efficient expression vector for transgenic medaka construction." @default.
- W1596411128 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8000477" @default.
- W1596411128 hasPublicationYear "1994" @default.
- W1596411128 type Work @default.
- W1596411128 sameAs 1596411128 @default.
- W1596411128 citedByCount "34" @default.
- W1596411128 countsByYear W15964111282015 @default.
- W1596411128 countsByYear W15964111282018 @default.
- W1596411128 countsByYear W15964111282020 @default.
- W1596411128 crossrefType "journal-article" @default.
- W1596411128 hasAuthorship W1596411128A5029737800 @default.
- W1596411128 hasAuthorship W1596411128A5044136355 @default.
- W1596411128 hasAuthorship W1596411128A5063467418 @default.
- W1596411128 hasAuthorship W1596411128A5065901351 @default.
- W1596411128 hasAuthorship W1596411128A5074930965 @default.
- W1596411128 hasAuthorship W1596411128A5075718612 @default.
- W1596411128 hasAuthorship W1596411128A5086839055 @default.
- W1596411128 hasConcept C100012643 @default.
- W1596411128 hasConcept C102230213 @default.
- W1596411128 hasConcept C104317684 @default.
- W1596411128 hasConcept C111936080 @default.
- W1596411128 hasConcept C142575336 @default.
- W1596411128 hasConcept C142613039 @default.
- W1596411128 hasConcept C150194340 @default.
- W1596411128 hasConcept C153911025 @default.
- W1596411128 hasConcept C161733203 @default.
- W1596411128 hasConcept C196843134 @default.
- W1596411128 hasConcept C2777289358 @default.
- W1596411128 hasConcept C33161422 @default.
- W1596411128 hasConcept C40767141 @default.
- W1596411128 hasConcept C54355233 @default.
- W1596411128 hasConcept C86803240 @default.
- W1596411128 hasConcept C92087593 @default.
- W1596411128 hasConcept C95444343 @default.
- W1596411128 hasConceptScore W1596411128C100012643 @default.
- W1596411128 hasConceptScore W1596411128C102230213 @default.
- W1596411128 hasConceptScore W1596411128C104317684 @default.
- W1596411128 hasConceptScore W1596411128C111936080 @default.
- W1596411128 hasConceptScore W1596411128C142575336 @default.
- W1596411128 hasConceptScore W1596411128C142613039 @default.
- W1596411128 hasConceptScore W1596411128C150194340 @default.
- W1596411128 hasConceptScore W1596411128C153911025 @default.
- W1596411128 hasConceptScore W1596411128C161733203 @default.
- W1596411128 hasConceptScore W1596411128C196843134 @default.
- W1596411128 hasConceptScore W1596411128C2777289358 @default.
- W1596411128 hasConceptScore W1596411128C33161422 @default.
- W1596411128 hasConceptScore W1596411128C40767141 @default.
- W1596411128 hasConceptScore W1596411128C54355233 @default.
- W1596411128 hasConceptScore W1596411128C86803240 @default.
- W1596411128 hasConceptScore W1596411128C92087593 @default.
- W1596411128 hasConceptScore W1596411128C95444343 @default.
- W1596411128 hasIssue "4" @default.
- W1596411128 hasLocation W15964111281 @default.
- W1596411128 hasOpenAccess W1596411128 @default.
- W1596411128 hasPrimaryLocation W15964111281 @default.
- W1596411128 hasRelatedWork W1596411128 @default.
- W1596411128 hasRelatedWork W1978135902 @default.
- W1596411128 hasRelatedWork W2047468933 @default.
- W1596411128 hasRelatedWork W2166784182 @default.
- W1596411128 hasRelatedWork W2347882378 @default.
- W1596411128 hasRelatedWork W2366017690 @default.
- W1596411128 hasRelatedWork W2380925533 @default.
- W1596411128 hasRelatedWork W2387677942 @default.
- W1596411128 hasRelatedWork W2438912532 @default.
- W1596411128 hasRelatedWork W2521630610 @default.
- W1596411128 hasVolume "3" @default.
- W1596411128 isParatext "false" @default.
- W1596411128 isRetracted "false" @default.
- W1596411128 magId "1596411128" @default.
- W1596411128 workType "article" @default.