FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1596570698> ?p ?o ?g. }

• W1596570698 endingPage "716" @default.
• W1596570698 startingPage "709" @default.
• W1596570698 abstract "Purple acid phosphatase from sweet potatoes Ipomoea batatas (spPAP) has been purified to homogeneity and characterized using spectroscopic investigations. Matrix-assisted laser desorption/ionization mass spectrometry analysis revealed a molecular mass of approximately 112 kDa. The metal content was determined by X-ray fluorescence using synchrotron radiation. In contrast to previous studies it is shown that spPAP contains a Fe(III)-Zn(II) center in the active site as previously determined for the purple acid phosphatase from red kidney bean (kbPAP). Moreover, an alignment of the amino acid sequences suggests that the residues involved in metal-binding are identical in both plant PAPs. Tyrosine functions as one of the ligands for the chromophoric Fe(III). Low temperature EPR spectra of spPAP show a signal near g = 4.3, characteristic for high-spin Fe(III) in a rhombic environment. The Tyr-Fe(III) charge transfer transition and the EPR signal are both very sensitive to changes in pH. The pH dependency strongly suggests the presence of an ionizable group with a pKa of 4.7, arising from an aquo ligand coordinated to Fe(III). EPR and UV/visible studies of spPAP in the presence of the inhibitors phosphate or arsenate suggest that both anions bind to Fe(III) in the binuclear center replacing the coordinated water or hydroxide ligand necessary for hydrolysis. The conserved histidine residues of spPAP corresponding to His202 and His296 in kbPAP probably interact in catalysis." @default.
• W1596570698 created "2016-06-24" @default.
• W1596570698 creator A5023155028 @default.
• W1596570698 creator A5031720787 @default.
• W1596570698 creator A5044326170 @default.
• W1596570698 creator A5074862311 @default.
• W1596570698 creator A5076097399 @default.
• W1596570698 creator A5082125519 @default.
• W1596570698 creator A5083164737 @default.
• W1596570698 date "1999-03-05" @default.
• W1596570698 modified "2023-09-27" @default.
• W1596570698 title "The active site of purple acid phosphatase from sweet potatoes (Ipomoea batatas) . Metal content and spectroscopic characterization" @default.
• W1596570698 cites W1502153098 @default.
• W1596570698 cites W154228731 @default.
• W1596570698 cites W1554724146 @default.
• W1596570698 cites W1572305446 @default.
• W1596570698 cites W1582923620 @default.
• W1596570698 cites W1608092708 @default.
• W1596570698 cites W1681781823 @default.
• W1596570698 cites W1757518583 @default.
• W1596570698 cites W1882216485 @default.
• W1596570698 cites W1915229566 @default.
• W1596570698 cites W1963821971 @default.
• W1596570698 cites W1968592306 @default.
• W1596570698 cites W1978493698 @default.
• W1596570698 cites W1979896323 @default.
• W1596570698 cites W1989019120 @default.
• W1596570698 cites W1989980254 @default.
• W1596570698 cites W1993361317 @default.
• W1596570698 cites W2013668535 @default.
• W1596570698 cites W2016097286 @default.
• W1596570698 cites W2019886699 @default.
• W1596570698 cites W2020386376 @default.
• W1596570698 cites W2021770929 @default.
• W1596570698 cites W2024269893 @default.
• W1596570698 cites W2024461057 @default.
• W1596570698 cites W2024469777 @default.
• W1596570698 cites W2028700212 @default.
• W1596570698 cites W2032430513 @default.
• W1596570698 cites W2036342654 @default.
• W1596570698 cites W2050021035 @default.
• W1596570698 cites W2056972905 @default.
• W1596570698 cites W2066560589 @default.
• W1596570698 cites W2067373519 @default.
• W1596570698 cites W2067601423 @default.
• W1596570698 cites W2073546925 @default.
• W1596570698 cites W2088498241 @default.
• W1596570698 cites W2091897331 @default.
• W1596570698 cites W2092672139 @default.
• W1596570698 cites W2093881465 @default.
• W1596570698 cites W2100837269 @default.
• W1596570698 cites W2107228249 @default.
• W1596570698 cites W2150494686 @default.
• W1596570698 cites W2150675804 @default.
• W1596570698 cites W2154648316 @default.
• W1596570698 cites W2303011402 @default.
• W1596570698 cites W2440740512 @default.
• W1596570698 cites W2951737999 @default.
• W1596570698 cites W411595376 @default.
• W1596570698 doi "https://doi.org/10.1046/j.1432-1327.1999.00230.x" @default.
• W1596570698 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10102999" @default.
• W1596570698 hasPublicationYear "1999" @default.
• W1596570698 type Work @default.
• W1596570698 sameAs 1596570698 @default.
• W1596570698 citedByCount "92" @default.
• W1596570698 countsByYear W15965706982012 @default.
• W1596570698 countsByYear W15965706982013 @default.
• W1596570698 countsByYear W15965706982014 @default.
• W1596570698 countsByYear W15965706982015 @default.
• W1596570698 countsByYear W15965706982016 @default.
• W1596570698 countsByYear W15965706982018 @default.
• W1596570698 countsByYear W15965706982019 @default.
• W1596570698 countsByYear W15965706982020 @default.
• W1596570698 countsByYear W15965706982021 @default.
• W1596570698 countsByYear W15965706982022 @default.
• W1596570698 countsByYear W15965706982023 @default.
• W1596570698 crossrefType "journal-article" @default.
• W1596570698 hasAuthorship W1596570698A5023155028 @default.
• W1596570698 hasAuthorship W1596570698A5031720787 @default.
• W1596570698 hasAuthorship W1596570698A5044326170 @default.
• W1596570698 hasAuthorship W1596570698A5074862311 @default.
• W1596570698 hasAuthorship W1596570698A5076097399 @default.
• W1596570698 hasAuthorship W1596570698A5082125519 @default.
• W1596570698 hasAuthorship W1596570698A5083164737 @default.
• W1596570698 hasConcept C116569031 @default.
• W1596570698 hasConcept C121332964 @default.
• W1596570698 hasConcept C13965031 @default.
• W1596570698 hasConcept C170493617 @default.
• W1596570698 hasConcept C179104552 @default.
• W1596570698 hasConcept C181199279 @default.
• W1596570698 hasConcept C185592680 @default.
• W1596570698 hasConcept C187961010 @default.
• W1596570698 hasConcept C2775896084 @default.
• W1596570698 hasConcept C2778460671 @default.
• W1596570698 hasConcept C41183919 @default.
• W1596570698 hasConcept C46141821 @default.
• W1596570698 hasConcept C515207424 @default.
• W1596570698 hasConcept C55493867 @default.

• next