Matches in SemOpenAlex for { <https://semopenalex.org/work/W1597156601> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W1597156601 endingPage "871" @default.
- W1597156601 startingPage "868" @default.
- W1597156601 abstract "Lys15 in Escherichia coli glycogen synthase, which is specifically labeled by adenosine diphosphopyridoxal, is mainly involved in binding of the substrate ADP-glucose (Furukawa, K., Tagaya, M., Tanizawa, K., and Fukui, T. (1993) J. Biol. Chem. 268, 23837-23842). We have found that the mutant glycogen synthase in which Lys15 is replaced by Gln via site-directed mutagenesis is inactivated by adenosine diphosphopyridoxal at concentrations higher than those required for the inactivation of the wild-type enzyme. ADP and ADP-glucose offered protective effects on inactivation, suggesting that the label binds to the ADP-glucose-binding site in the mutant enzyme. Sequence analysis of the labeled peptide revealed that the labeled residue is Lys277. This lysyl residue is conserved in maize starch synthase, which shows about 30% amino acid identity to E. coli glycogen synthase. Substitution of Gln for Lys277 by site-directed mutagenesis resulted in a 140-fold decrease in the kcat value with little changes in the Km values for ADP-glucose and glycogen. These results suggest that Lys277 at the active site participates in the catalytic reaction rather than binding of substrate. The present study shows the usefulness of the combined application of affinity labeling and site-directed mutagenesis." @default.
- W1597156601 created "2016-06-24" @default.
- W1597156601 creator A5014155960 @default.
- W1597156601 creator A5027831160 @default.
- W1597156601 creator A5041595446 @default.
- W1597156601 creator A5043738258 @default.
- W1597156601 date "1994-01-01" @default.
- W1597156601 modified "2023-10-17" @default.
- W1597156601 title "Identification of Lys277 at the active site of Escherichia coli glycogen synthase. Application of affinity labeling combined with site-directed mutagenesis." @default.
- W1597156601 cites W1479783860 @default.
- W1597156601 cites W1489140179 @default.
- W1597156601 cites W1510429270 @default.
- W1597156601 cites W1530881561 @default.
- W1597156601 cites W1531991603 @default.
- W1597156601 cites W1580223847 @default.
- W1597156601 cites W1609933325 @default.
- W1597156601 cites W1657860793 @default.
- W1597156601 cites W1972663144 @default.
- W1597156601 cites W2001866120 @default.
- W1597156601 cites W2034138247 @default.
- W1597156601 cites W2041235076 @default.
- W1597156601 cites W2062384551 @default.
- W1597156601 cites W2065335949 @default.
- W1597156601 cites W2070539114 @default.
- W1597156601 cites W2072374953 @default.
- W1597156601 cites W2079229201 @default.
- W1597156601 cites W2138270253 @default.
- W1597156601 cites W4293247451 @default.
- W1597156601 doi "https://doi.org/10.1016/s0021-9258(17)42192-2" @default.
- W1597156601 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8288640" @default.
- W1597156601 hasPublicationYear "1994" @default.
- W1597156601 type Work @default.
- W1597156601 sameAs 1597156601 @default.
- W1597156601 citedByCount "27" @default.
- W1597156601 countsByYear W15971566012014 @default.
- W1597156601 countsByYear W15971566012016 @default.
- W1597156601 countsByYear W15971566012017 @default.
- W1597156601 countsByYear W15971566012018 @default.
- W1597156601 crossrefType "journal-article" @default.
- W1597156601 hasAuthorship W1597156601A5014155960 @default.
- W1597156601 hasAuthorship W1597156601A5027831160 @default.
- W1597156601 hasAuthorship W1597156601A5041595446 @default.
- W1597156601 hasAuthorship W1597156601A5043738258 @default.
- W1597156601 hasBestOaLocation W15971566011 @default.
- W1597156601 hasConcept C103408237 @default.
- W1597156601 hasConcept C104317684 @default.
- W1597156601 hasConcept C107824862 @default.
- W1597156601 hasConcept C112243037 @default.
- W1597156601 hasConcept C116834253 @default.
- W1597156601 hasConcept C143065580 @default.
- W1597156601 hasConcept C16318435 @default.
- W1597156601 hasConcept C181199279 @default.
- W1597156601 hasConcept C185592680 @default.
- W1597156601 hasConcept C192118531 @default.
- W1597156601 hasConcept C41183919 @default.
- W1597156601 hasConcept C501734568 @default.
- W1597156601 hasConcept C547475151 @default.
- W1597156601 hasConcept C55493867 @default.
- W1597156601 hasConcept C59822182 @default.
- W1597156601 hasConcept C86803240 @default.
- W1597156601 hasConceptScore W1597156601C103408237 @default.
- W1597156601 hasConceptScore W1597156601C104317684 @default.
- W1597156601 hasConceptScore W1597156601C107824862 @default.
- W1597156601 hasConceptScore W1597156601C112243037 @default.
- W1597156601 hasConceptScore W1597156601C116834253 @default.
- W1597156601 hasConceptScore W1597156601C143065580 @default.
- W1597156601 hasConceptScore W1597156601C16318435 @default.
- W1597156601 hasConceptScore W1597156601C181199279 @default.
- W1597156601 hasConceptScore W1597156601C185592680 @default.
- W1597156601 hasConceptScore W1597156601C192118531 @default.
- W1597156601 hasConceptScore W1597156601C41183919 @default.
- W1597156601 hasConceptScore W1597156601C501734568 @default.
- W1597156601 hasConceptScore W1597156601C547475151 @default.
- W1597156601 hasConceptScore W1597156601C55493867 @default.
- W1597156601 hasConceptScore W1597156601C59822182 @default.
- W1597156601 hasConceptScore W1597156601C86803240 @default.
- W1597156601 hasIssue "2" @default.
- W1597156601 hasLocation W15971566011 @default.
- W1597156601 hasOpenAccess W1597156601 @default.
- W1597156601 hasPrimaryLocation W15971566011 @default.
- W1597156601 hasRelatedWork W2024647076 @default.
- W1597156601 hasRelatedWork W2040122145 @default.
- W1597156601 hasRelatedWork W2349738535 @default.
- W1597156601 hasRelatedWork W2358175167 @default.
- W1597156601 hasRelatedWork W2365402183 @default.
- W1597156601 hasRelatedWork W2528862892 @default.
- W1597156601 hasRelatedWork W2565847177 @default.
- W1597156601 hasRelatedWork W2754688931 @default.
- W1597156601 hasRelatedWork W294129591 @default.
- W1597156601 hasRelatedWork W4246966729 @default.
- W1597156601 hasVolume "269" @default.
- W1597156601 isParatext "false" @default.
- W1597156601 isRetracted "false" @default.
- W1597156601 magId "1597156601" @default.
- W1597156601 workType "article" @default.