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- W1597951212 abstract "Abstract When examined, the expansion of many stem cell classes has been shown to be facilitated by mechanically‐regulated calcium entry from the extracellular space that also helps direct their developmental programs towards mechanosensitive tissues such as muscle, bone, and connective tissues. Cation channels of the transient receptor potential C class (TRPC) are the predominant conduit for calcium entry into proliferating myoblasts. Nonetheless, methods to non‐invasively study this calcium‐entry pathway are still in their infancy. Here we show that a microfluidic configuration of impedance‐based flow cytometry (IFC) provides a method to detect TRP channel expression in cells at high throughput. Using this technology we discern changes in the IFC signal that correlates with the functional expression of TRPC1 channels and coincides with cell proliferation. Pharmacological agents, mechanical conditions or malignant states that alter the expression of TRPC1 channels are reflected in the IFC signal accordingly, whereas pharmacological agents that alter cation‐permeation through TRPC1 channels, or ionophores that independently increase calcium entry across the membrane, have little effect. Our results suggest that IFC detects changes in whole‐cell membrane organization associated with TRPC1 activation and surface expression, rather than cation permeation through the channel per se. IFC‐based technologies thus have the potential to identify living stem cells in their earliest stages of expansion without staining or chemical fixation. © 2014 International Society for Advancement of Cytometry" @default.
- W1597951212 created "2016-06-24" @default.
- W1597951212 creator A5019698938 @default.
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- W1597951212 creator A5059435292 @default.
- W1597951212 creator A5060937441 @default.
- W1597951212 creator A5063360982 @default.
- W1597951212 creator A5063517746 @default.
- W1597951212 creator A5071881588 @default.
- W1597951212 creator A5089727922 @default.
- W1597951212 date "2014-03-17" @default.
- W1597951212 modified "2023-10-16" @default.
- W1597951212 title "Impedance flow cytometry gauges proliferative capacity by detecting TRPC1 expression" @default.
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