FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1598156923> ?p ?o ?g. }

• W1598156923 endingPage "315" @default.
• W1598156923 startingPage "309" @default.
• W1598156923 abstract "Abstract Glucagon‐like peptide‐1 (GLP‐1) plays an important role in the regulation of postprandial insulin release. Here, we used the split DnaB mini‐intein system to produce recombinant human GLP‐1/7‐36 (rhGLP‐1) in Escherichia coli . The C‐terminal domain of DnaB mini‐intein (IntC) was genetically fused at the N‐terminus of rhGLP‐1 to produce IntC–GLP‐1. IntC–GLP‐1 and N‐terminal domain of DnaB mini‐intein (IntN) protein were prepared in a denatured buffer of pH 8.0. IntC–GLP‐1 was diluted 1:8 into the phosphate buffer of pH 6.6. IntN was added into the diluted solution of IntC–GLP‐1 at the molar ratio of 1:2. Then, rhGLP‐1 was released from IntC–GLP‐1 via inducible C‐terminal peptide‐bond cleavage by shifting pH from 8.0 to 6.6 at 25 °C for 24‐H incubation. Then, the supernatant was applied to a Ni‐Sepharose column, and the pass through fraction was collected. About 5.34 mg of rhGLP‐1 with the purity of 97% was obtained from 1 L of culture medium. Mass spectrometry showed the molecular weight of 3,300.45 Da, which was equal to the theoretical value of GLP‐1/7‐36. The glucose‐lowering activity of rhGLP‐1 was confirmed by the glucose tolerance test in mice. In conclusion, the reported method was an efficient strategy to produce rhGLP‐1 without using enzyme or chemical reagents, which could also be used for other similar peptides." @default.
• W1598156923 created "2016-06-24" @default.
• W1598156923 creator A5032001213 @default.
• W1598156923 creator A5038068584 @default.
• W1598156923 creator A5050110637 @default.
• W1598156923 creator A5059589652 @default.
• W1598156923 creator A5079515531 @default.
• W1598156923 creator A5088708037 @default.
• W1598156923 date "2015-01-02" @default.
• W1598156923 modified "2023-10-17" @default.
• W1598156923 title "Split Ssp DnaB mini-intein-mediated production of recombinant human glucagon-like peptide-1/7-36" @default.
• W1598156923 cites W1484278934 @default.
• W1598156923 cites W1964117837 @default.
• W1598156923 cites W1966967447 @default.
• W1598156923 cites W1967149839 @default.
• W1598156923 cites W1967383069 @default.
• W1598156923 cites W1968823764 @default.
• W1598156923 cites W1969932276 @default.
• W1598156923 cites W1971433358 @default.
• W1598156923 cites W1973214902 @default.
• W1598156923 cites W2002989289 @default.
• W1598156923 cites W2004891119 @default.
• W1598156923 cites W2034574623 @default.
• W1598156923 cites W2039564527 @default.
• W1598156923 cites W2039642467 @default.
• W1598156923 cites W2047887710 @default.
• W1598156923 cites W2074073503 @default.
• W1598156923 cites W2080128749 @default.
• W1598156923 cites W2087147260 @default.
• W1598156923 cites W2087751600 @default.
• W1598156923 cites W2108420279 @default.
• W1598156923 cites W2109050242 @default.
• W1598156923 cites W2111216775 @default.
• W1598156923 cites W2124182221 @default.
• W1598156923 cites W2139111319 @default.
• W1598156923 cites W2146581079 @default.
• W1598156923 cites W2151589307 @default.
• W1598156923 cites W2165928631 @default.
• W1598156923 doi "https://doi.org/10.1002/bab.1274" @default.
• W1598156923 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25066911" @default.
• W1598156923 hasPublicationYear "2015" @default.
• W1598156923 type Work @default.
• W1598156923 sameAs 1598156923 @default.
• W1598156923 citedByCount "6" @default.
• W1598156923 countsByYear W15981569232015 @default.
• W1598156923 countsByYear W15981569232016 @default.
• W1598156923 countsByYear W15981569232017 @default.
• W1598156923 countsByYear W15981569232019 @default.
• W1598156923 countsByYear W15981569232021 @default.
• W1598156923 crossrefType "journal-article" @default.
• W1598156923 hasAuthorship W1598156923A5032001213 @default.
• W1598156923 hasAuthorship W1598156923A5038068584 @default.
• W1598156923 hasAuthorship W1598156923A5050110637 @default.
• W1598156923 hasAuthorship W1598156923A5059589652 @default.
• W1598156923 hasAuthorship W1598156923A5079515531 @default.
• W1598156923 hasAuthorship W1598156923A5088708037 @default.
• W1598156923 hasConcept C104317684 @default.
• W1598156923 hasConcept C116107829 @default.
• W1598156923 hasConcept C134018914 @default.
• W1598156923 hasConcept C151730666 @default.
• W1598156923 hasConcept C161223559 @default.
• W1598156923 hasConcept C175156509 @default.
• W1598156923 hasConcept C181199279 @default.
• W1598156923 hasConcept C185592680 @default.
• W1598156923 hasConcept C2775954774 @default.
• W1598156923 hasConcept C2776398474 @default.
• W1598156923 hasConcept C2777180221 @default.
• W1598156923 hasConcept C2779281246 @default.
• W1598156923 hasConcept C40767141 @default.
• W1598156923 hasConcept C43369102 @default.
• W1598156923 hasConcept C43617362 @default.
• W1598156923 hasConcept C54458228 @default.
• W1598156923 hasConcept C547475151 @default.
• W1598156923 hasConcept C55493867 @default.
• W1598156923 hasConcept C555293320 @default.
• W1598156923 hasConcept C67705224 @default.
• W1598156923 hasConcept C86803240 @default.
• W1598156923 hasConceptScore W1598156923C104317684 @default.
• W1598156923 hasConceptScore W1598156923C116107829 @default.
• W1598156923 hasConceptScore W1598156923C134018914 @default.
• W1598156923 hasConceptScore W1598156923C151730666 @default.
• W1598156923 hasConceptScore W1598156923C161223559 @default.
• W1598156923 hasConceptScore W1598156923C175156509 @default.
• W1598156923 hasConceptScore W1598156923C181199279 @default.
• W1598156923 hasConceptScore W1598156923C185592680 @default.
• W1598156923 hasConceptScore W1598156923C2775954774 @default.
• W1598156923 hasConceptScore W1598156923C2776398474 @default.
• W1598156923 hasConceptScore W1598156923C2777180221 @default.
• W1598156923 hasConceptScore W1598156923C2779281246 @default.
• W1598156923 hasConceptScore W1598156923C40767141 @default.
• W1598156923 hasConceptScore W1598156923C43369102 @default.
• W1598156923 hasConceptScore W1598156923C43617362 @default.
• W1598156923 hasConceptScore W1598156923C54458228 @default.
• W1598156923 hasConceptScore W1598156923C547475151 @default.
• W1598156923 hasConceptScore W1598156923C55493867 @default.
• W1598156923 hasConceptScore W1598156923C555293320 @default.
• W1598156923 hasConceptScore W1598156923C67705224 @default.
• W1598156923 hasConceptScore W1598156923C86803240 @default.

• next