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• W1598171260 abstract "The role of renal expression of the glycosphingolipid verotoxin receptor, globotriaosylceramide, in susceptibility to verotoxin-induced hemolytic uremic syndrome is unclear. We show that a single glycosphingolipid can discriminate multiple specific ligands. Antibody detection of globotriaosylceramide in renal sections does not necessarily predict verotoxin binding. The deoxyglobotriaosylceramide binding profile for verotoxin 1, verotoxin 2 and monoclonal anti-globotriaosylceramide are distinct. Anti-globotriaosylceramide had greater dependency on the intact alpha-galactose and reducing glucose of globotriaosylceramide than verotoxin 1, while verotoxin 2 was intermediate. These ligands differentially stained human kidney sections. Glomerulopathy is the primary verotoxin-associated pathology in hemolytic uremic syndrome. For most samples, verotoxin 1 immunostaining within adult glomeruli was observed (type A). Some samples, however, lacked glomerular binding (type B). Anti-globotriaosylceramide (and less effectively, verotoxin 2) stained all glomeruli. Verotoxin 1/anti-globotriaosylceramide tubular staining was comparable. Type B glomerular/tubular globotriaosylceramide showed minor, but significant, fatty acid compositional differences. Verotoxin 1 type B glomerular binding became evident following pretreatment with cold acetone, or methyl-beta-cyclodextrin, used to deplete cholesterol. Direct visualization, using fluorescein isothiocyanate-verotoxin 1B, showed paediatric, but no adult glomerular staining; this was confirmed by anti-fluorescein isothiocyanate immunostaining. Acetone induced fluorescein isothiocyanate-verotoxin 1B glomerular staining in type A, but poorly in type B samples. Comparison of fluorescein isothiocyanate-verotoxin 1B and native verotoxin 1B deoxyglobotriaosylceramide analogue binding showed an alteration in subspecificity. These studies indicate a marked heterogeneity of globotriaosylceramide expression within renal glomeruli and differential binding of verotoxin 1/verotoxin 2/anti-globotriaosylceramide to the same glycosphingolipid. Verotoxin 1 derivatization can induce subtle changes in globotriaosylceramide binding to significantly affect tissue binding. Heterogeneity in glomerular globotriaosylceramide expression may play a significant (cholesterol-dependent?) role in determining renal pathology following verotoxemia." @default.
• W1598171260 created "2016-06-24" @default.
• W1598171260 creator A5027697115 @default.
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• W1598171260 date "2004-01-01" @default.
• W1598171260 modified "2023-10-04" @default.
• W1598171260 title "Differential carbohydrate epitope recognition of globotriaosyl ceramide by verotoxins and a monoclonal antibody. Role in human renal glomerular binding" @default.
• W1598171260 cites W135718510 @default.
• W1598171260 cites W1514916393 @default.
• W1598171260 cites W1527640586 @default.
• W1598171260 cites W1532731856 @default.
• W1598171260 cites W1560132616 @default.
• W1598171260 cites W1566518730 @default.
• W1598171260 cites W1589876585 @default.
• W1598171260 cites W1845375977 @default.
• W1598171260 cites W187359374 @default.
• W1598171260 cites W1875725345 @default.
• W1598171260 cites W1904815041 @default.
• W1598171260 cites W1942715185 @default.
• W1598171260 cites W1955659686 @default.
• W1598171260 cites W1965399938 @default.
• W1598171260 cites W1968255815 @default.
• W1598171260 cites W1970674113 @default.
• W1598171260 cites W1971356206 @default.
• W1598171260 cites W1971454418 @default.
• W1598171260 cites W1972250242 @default.
• W1598171260 cites W1972562981 @default.
• W1598171260 cites W1975203999 @default.
• W1598171260 cites W1975239369 @default.
• W1598171260 cites W1975977355 @default.
• W1598171260 cites W1976155285 @default.
• W1598171260 cites W1980288845 @default.
• W1598171260 cites W1992888087 @default.
• W1598171260 cites W1994721067 @default.
• W1598171260 cites W1995610766 @default.
• W1598171260 cites W1997235040 @default.
• W1598171260 cites W1998980382 @default.
• W1598171260 cites W2000006041 @default.
• W1598171260 cites W2002296874 @default.
• W1598171260 cites W2003915689 @default.
• W1598171260 cites W2010756850 @default.
• W1598171260 cites W2013840662 @default.
• W1598171260 cites W2014903320 @default.
• W1598171260 cites W2022650596 @default.
• W1598171260 cites W2032304130 @default.
• W1598171260 cites W2032882661 @default.
• W1598171260 cites W2037363944 @default.
• W1598171260 cites W2037781176 @default.
• W1598171260 cites W2037812623 @default.
• W1598171260 cites W2043288373 @default.
• W1598171260 cites W2049055942 @default.
• W1598171260 cites W2051766718 @default.
• W1598171260 cites W2054192473 @default.
• W1598171260 cites W2058379009 @default.
• W1598171260 cites W2065105263 @default.
• W1598171260 cites W2066521555 @default.
• W1598171260 cites W2069209583 @default.
• W1598171260 cites W2075140645 @default.
• W1598171260 cites W2082799697 @default.
• W1598171260 cites W2083983934 @default.
• W1598171260 cites W2086433473 @default.
• W1598171260 cites W2088607589 @default.
• W1598171260 cites W2089015231 @default.
• W1598171260 cites W2094842645 @default.
• W1598171260 cites W2110885272 @default.
• W1598171260 cites W2113022428 @default.
• W1598171260 cites W2113219905 @default.
• W1598171260 cites W2113987232 @default.
• W1598171260 cites W2133501797 @default.
• W1598171260 cites W2135110183 @default.
• W1598171260 cites W2136344073 @default.
• W1598171260 cites W2138668166 @default.
• W1598171260 cites W2154870173 @default.
• W1598171260 cites W2156327171 @default.
• W1598171260 cites W2171557036 @default.
• W1598171260 cites W2253838792 @default.
• W1598171260 cites W2320536146 @default.
• W1598171260 cites W2333892303 @default.
• W1598171260 cites W2407755529 @default.
• W1598171260 cites W2410657234 @default.
• W1598171260 cites W252325056 @default.
• W1598171260 cites W333236270 @default.
• W1598171260 cites W4231242471 @default.
• W1598171260 cites W4234951362 @default.
• W1598171260 cites W64708402 @default.
• W1598171260 doi "https://doi.org/10.1046/j.1432-1033.2003.03941.x" @default.
• W1598171260 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14717708" @default.
• W1598171260 hasPublicationYear "2004" @default.
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