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• W1598173898 abstract "Dans le secteur biomedical et l’industrie agro-alimentaire, l’adhesion de microorganismes contaminants auxsurfaces engendre de multiples impacts negatifs, a la fois en termes de sante publique, d’hygiene et de securitealimentaire. Dans ce contexte, l’objectif de l’etude est de mettre au point un traitement de surface de l’acierinoxydable 316L, afin de prevenir la colonisation microbienne. La modification des surfaces d’acier partraitement chimique ou physique n’a eu aucune incidence sur le detachement de Saccharomyces cerevisiae,evalue in vitro a l’aide d’une chambre a ecoulement cisaille. Les interactions entre la surface microbienne et leselements metalliques du film passif semblent jouer un role preponderant dans cette forte adhesion. Unestrategie originale, basee sur un procede plasma couplant la polymerisation d’hexamethyldisiloxane aubombardement d’une cible d’argent dans une decharge asymetrique radiofrequence, a ensuite ete mise enoeuvre et optimisee. Les surfaces d’acier ont ainsi ete recouvertes de films minces (~ 175 nm)nanocomposites, constitues d’une matrice organosiliciee, presentant des proprietes anti-adhesives vis-a-vis deS. cerevisiae, dans laquelle ont ete incluses des nanoparticules d’argent, dotees d’une forte reactiviteantimicrobienne. Le couplage de techniques d’analyse complementaires, operant a differentes echelles, apermis de correler les caracteristiques des films nanocomposites a leur efficacite anti-adhesive et antifongique.Une inhibition totale de l’adhesion des levures a ainsi ete obtenue, en augmentant le caractere polaire de lamatrice, par ajout d’oxygene dans le plasma. En parallele, un abattement de la viabilite de 1,9 log a ete atteintsur les levures sessiles. La suite de l’etude a ete dediee a la comprehension des mecanismes d’action del’argent, impliques dans l’activite antifongique des films nanocomposites. Une inactivation de certaines proteinesparietales et intracellulaires, correlee a des alterations de l’ultra-structure cellulaire, a ainsi ete mise en evidence.La confirmation de l’activite biocide des films nanocomposites, sur deux modeles procaryotes (Staphylococcusaureus et Escherichia coli), a revele par ailleurs la necessite d’un contact etroit entre microorganismes etrevetement. Enfin, la stabilite des proprietes des films nanocomposites a ete evaluee. Une utilisation repeteedes depots a mis en evidence une reduction de l’activite antifongique, correlee a une augmentation del’efficacite anti-adhesive, liee au relargage d’argent lors de la premiere utilisation.----------------------------------------------------------------------------------------------------------------------------------------------------------------- In the biomedical domain and the food industry, microbial adhesion to surfaces generates multiple negativeconsequences, in terms of human health, hygiene and safety of processed food. In this context, our approachis based on developing a 316L stainless steel surface treatment, to prevent microbial colonization. The surfacemodifications, mediated by chemical or physical treatment, did not promote Saccharomyces cerevisiaedetachment, evaluated in vitro using a shear stress flow chamber. The interactions between the microbialsurface and metallic elements of the passive film were hypothesized to play a predominant role in this strongadhesion. An original and dual strategy, based on a plasma process associating hexamethyldisiloxanepolymerization and silver target bombardment in an asymmetrical radiofrequency discharge, was carried outand optimized. Stainless steel surfaces were thus coated with nanocomposite thin films (~ 175 nm), composedof an organosilicon matrix, exhibiting anti-adhesive properties towards S. cerevisiae, in which were embeddedsilver nanoparticles, displaying a high antimicrobial reactivity. A large set of complementary analyticaltechniques, operating at different scales, was used to correlate nanocomposite film characteristics with theiranti-adhesive and antimicrobial efficiency. A total inhibition of yeast cell adhesion was achieved, by increasingthe matrix polar character, through oxygen addition during the plasma process. In parallel, a 1.9 log reductionin viable counts was achieved for sessile yeast cells. Further experiments were dedicated to the thoroughunderstanding of cellular changes induced by silver release. A deterioration of the secondary structure ofproteins (cell wall, intracellular), combined with ultra-structure alterations, was observed. In addition, thebiocide activity of the nanocomposite film was confirmed against two prokaryotic models (Staphylococcusaureus and Escherichia coli). The necessity of a direct contact between microorganisms and coating wasdemonstrated for a maximal antimicrobial efficiency. Lastly, the durability of the coating properties wasassessed through a repeated use of the nanocomposite films. A decrease in the antifungal activity, coupled toan anti-adhesive property enhancement, was noticed and explained by the silver release during the first use." @default.
• W1598173898 created "2016-06-24" @default.
• W1598173898 creator A5046277573 @default.
• W1598173898 date "2009-12-09" @default.
• W1598173898 modified "2023-09-24" @default.
• W1598173898 title "Evaluation des propriétés anti-adhésives et biocides de films nanocomposites avec inclusions d’argent, déposés sur acier inoxydable par procédé plasma" @default.
• W1598173898 hasPublicationYear "2009" @default.
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