Matches in SemOpenAlex for { <https://semopenalex.org/work/W1598536618> ?p ?o ?g. }
- W1598536618 endingPage "680" @default.
- W1598536618 startingPage "672" @default.
- W1598536618 abstract "Human IgG is produced with C-terminal lysines that are cleaved off in circulation. The function of this modification was unknown and generally thought not to affect antibody function. We recently reported that efficient C1q binding and complement-dependent cytotoxicity (CDC) requires IgG hexamerization at the cell surface. Here we demonstrate that C-terminal lysines may interfere with this process, leading to suboptimal C1q binding and CDC of cells opsonized with C-terminal lysine-containing IgG. After we removed these lysines with a carboxypeptidase, maximal complement activation was observed. Interestingly, IgG1 mutants containing either a negative C-terminal charge or multiple positive charges lost CDC almost completely; however, CDC was fully restored by mixing C-terminal mutants of opposite charge. Our data indicate a novel post-translational control mechanism of human IgG: human IgG molecules are produced in a pro-form in which charged C-termini interfere with IgG hexamer formation, C1q binding and CDC. To allow maximal complement activation, C-terminal lysine processing is required to release the antibody's full cytotoxic potential." @default.
- W1598536618 created "2016-06-24" @default.
- W1598536618 creator A5006107477 @default.
- W1598536618 creator A5009413569 @default.
- W1598536618 creator A5013693987 @default.
- W1598536618 creator A5016951786 @default.
- W1598536618 creator A5026647738 @default.
- W1598536618 creator A5033110998 @default.
- W1598536618 creator A5039190287 @default.
- W1598536618 creator A5070458905 @default.
- W1598536618 creator A5074865442 @default.
- W1598536618 creator A5087586287 @default.
- W1598536618 creator A5088697164 @default.
- W1598536618 date "2015-06-02" @default.
- W1598536618 modified "2023-10-14" @default.
- W1598536618 title "Human IgG is produced in a pro-form that requires clipping of C-terminal lysines for maximal complement activation" @default.
- W1598536618 cites W1455269369 @default.
- W1598536618 cites W1969482494 @default.
- W1598536618 cites W1970891956 @default.
- W1598536618 cites W1972913960 @default.
- W1598536618 cites W1974070052 @default.
- W1598536618 cites W1979419663 @default.
- W1598536618 cites W1979626824 @default.
- W1598536618 cites W1985960971 @default.
- W1598536618 cites W1986197388 @default.
- W1598536618 cites W1993370938 @default.
- W1598536618 cites W1993454294 @default.
- W1598536618 cites W1998829729 @default.
- W1598536618 cites W1998860271 @default.
- W1598536618 cites W2030522563 @default.
- W1598536618 cites W2039484130 @default.
- W1598536618 cites W2043228552 @default.
- W1598536618 cites W2050754173 @default.
- W1598536618 cites W2061518680 @default.
- W1598536618 cites W2065303540 @default.
- W1598536618 cites W2066856346 @default.
- W1598536618 cites W2074975150 @default.
- W1598536618 cites W2083423150 @default.
- W1598536618 cites W2084731542 @default.
- W1598536618 cites W2085141423 @default.
- W1598536618 cites W2087638630 @default.
- W1598536618 cites W2088539994 @default.
- W1598536618 cites W2089303592 @default.
- W1598536618 cites W2091229935 @default.
- W1598536618 cites W2092638671 @default.
- W1598536618 cites W2099684692 @default.
- W1598536618 cites W2127552260 @default.
- W1598536618 cites W2131928227 @default.
- W1598536618 cites W2134566626 @default.
- W1598536618 cites W2140604498 @default.
- W1598536618 cites W2141139539 @default.
- W1598536618 cites W2141502099 @default.
- W1598536618 cites W2147639736 @default.
- W1598536618 cites W2158803162 @default.
- W1598536618 cites W2159423922 @default.
- W1598536618 cites W2165398738 @default.
- W1598536618 cites W2166126901 @default.
- W1598536618 doi "https://doi.org/10.1080/19420862.2015.1046665" @default.
- W1598536618 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4622059" @default.
- W1598536618 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26037225" @default.
- W1598536618 hasPublicationYear "2015" @default.
- W1598536618 type Work @default.
- W1598536618 sameAs 1598536618 @default.
- W1598536618 citedByCount "54" @default.
- W1598536618 countsByYear W15985366182015 @default.
- W1598536618 countsByYear W15985366182016 @default.
- W1598536618 countsByYear W15985366182017 @default.
- W1598536618 countsByYear W15985366182018 @default.
- W1598536618 countsByYear W15985366182019 @default.
- W1598536618 countsByYear W15985366182020 @default.
- W1598536618 countsByYear W15985366182021 @default.
- W1598536618 countsByYear W15985366182022 @default.
- W1598536618 countsByYear W15985366182023 @default.
- W1598536618 crossrefType "journal-article" @default.
- W1598536618 hasAuthorship W1598536618A5006107477 @default.
- W1598536618 hasAuthorship W1598536618A5009413569 @default.
- W1598536618 hasAuthorship W1598536618A5013693987 @default.
- W1598536618 hasAuthorship W1598536618A5016951786 @default.
- W1598536618 hasAuthorship W1598536618A5026647738 @default.
- W1598536618 hasAuthorship W1598536618A5033110998 @default.
- W1598536618 hasAuthorship W1598536618A5039190287 @default.
- W1598536618 hasAuthorship W1598536618A5070458905 @default.
- W1598536618 hasAuthorship W1598536618A5074865442 @default.
- W1598536618 hasAuthorship W1598536618A5087586287 @default.
- W1598536618 hasAuthorship W1598536618A5088697164 @default.
- W1598536618 hasBestOaLocation W15985366181 @default.
- W1598536618 hasConcept C104317684 @default.
- W1598536618 hasConcept C109316439 @default.
- W1598536618 hasConcept C111684460 @default.
- W1598536618 hasConcept C12554922 @default.
- W1598536618 hasConcept C138873322 @default.
- W1598536618 hasConcept C143065580 @default.
- W1598536618 hasConcept C153911025 @default.
- W1598536618 hasConcept C159654299 @default.
- W1598536618 hasConcept C171279029 @default.
- W1598536618 hasConcept C185592680 @default.
- W1598536618 hasConcept C189754071 @default.
- W1598536618 hasConcept C202751555 @default.