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• W159884039 abstract "Research Article3 July 1995free access A potassium transporter of the yeast Schwanniomyces occidentalis homologous to the Kup system of Escherichia coli has a high concentrative capacity. M.A. Bañuelos M.A. Bañuelos Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, Madrid, Spain. Search for more papers by this author R.D. Klein R.D. Klein Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, Madrid, Spain. Search for more papers by this author S.J. Alexander-Bowman S.J. Alexander-Bowman Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, Madrid, Spain. Search for more papers by this author A. Rodríguez-Navarro A. Rodríguez-Navarro Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, Madrid, Spain. Search for more papers by this author M.A. Bañuelos M.A. Bañuelos Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, Madrid, Spain. Search for more papers by this author R.D. Klein R.D. Klein Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, Madrid, Spain. Search for more papers by this author S.J. Alexander-Bowman S.J. Alexander-Bowman Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, Madrid, Spain. Search for more papers by this author A. Rodríguez-Navarro A. Rodríguez-Navarro Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, Madrid, Spain. Search for more papers by this author Author Information M.A. Bañuelos1, R.D. Klein1, S.J. Alexander-Bowman1 and A. Rodríguez-Navarro1 1Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, Madrid, Spain. The EMBO Journal (1995)14:3021-3027https://doi.org/10.1002/j.1460-2075.1995.tb07304.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The yeast Schwanniomyces occidentalis has a high-affinity K+ uptake system with a high concentrative capacity, which is able to deplete the external K+ to < 0.03 microM. We have cloned the gene HAK1 of S.occidentalis which complements defective K+ uptake by trk1 and trk1 trk2 mutants of Saccharomyces cerevisiae. When HAK1 was expressed in a trk1 trk2 S.cerevisiae mutant, transport affinities for K+ and other alkali cations resembled those of S.occidentalis. The predicted amino acid sequence of the HAK1 protein shows significant homology with the hydrophobic region of the Kup transporter of Escherichia coli. In S.occidentalis HAK1 expresses in K(+)-limiting conditions. Our data indicate that in K(+)-starved cells the system encoded by HAK1 is the major K+ transporter of S.occidentalis. Previous ArticleNext Article Volume 14Issue 131 July 1995In this issue RelatedDetailsLoading ..." @default.
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• W159884039 title "A potassium transporter of the yeast Schwanniomyces occidentalis homologous to the Kup system of Escherichia coli has a high concentrative capacity." @default.
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