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- W1599915175 abstract "Abstract Whole‐genome amplification by multiple displacement amplification (MDA) is a patented method to generate potentially unlimited genomic material when researchers are challenged with trace samples, or the amount of genomic DNA required for analysis exceeds the amount on hand. It is an isothermal reaction, using Phi29 DNA polymerase and random hexamer primers for unbiased amplification of linear DNA molecules, such as genomic DNA. The random‐primed MDA reaction provides extensive amplification coverage of the genome, generates extremely long DNA products, and provides high DNA yields. This unit explains the reaction, and describes use of the commercial kits available. Curr. Protoc. Mol. Biol . 105:15.13.1‐15.13.16. © 2014 by John Wiley & Sons, Inc." @default.
- W1599915175 created "2016-06-24" @default.
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- W1599915175 date "2014-01-01" @default.
- W1599915175 modified "2023-09-30" @default.
- W1599915175 title "Random‐Primed, Phi29 DNA Polymerase‐Based Whole Genome Amplification" @default.
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- W1599915175 doi "https://doi.org/10.1002/0471142727.mb1513s105" @default.
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