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• W1600901894 abstract "The L-rhamnose-H+ symporter (RhaT) is a 344-amino acid integral membrane protein, found in many Enterobacteria, which couples the uptake of the sugar L-rhamnose with the inward movement of protons. Based on its hydropathy profile and the application of von Heijne's positive inside rule (von Heijne, G. (1992) J. Mol. Biol. 225, 487-494), a model of the L-rhamnose-H+ symport protein (RhaT) is proposed containing 10 transmembrane helices with the NH2 and COOH termini in the periplasm. This model was tested by the creation of random beta-lactamase (Bla) fusions. The data from 33 unique, randomly generated, RhaT-Bla fusions and from 5 site-specific fusions supported the proposed topology between transmembrane helices 2-10. However, the localization of the putative first hydrophilic loop and the NH2 terminus was not possible because the beta-lactamase fusions in this region were shown to be unreliable indicators of the topology of RhaT." @default.
• W1600901894 created "2016-06-24" @default.
• W1600901894 creator A5055099992 @default.
• W1600901894 creator A5087200267 @default.
• W1600901894 date "1993-12-01" @default.
• W1600901894 modified "2023-09-30" @default.
• W1600901894 title "Membrane topology of the L-rhamnose-H+ transport protein (RhaT) from enterobacteria." @default.
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• W1600901894 doi "https://doi.org/10.1016/s0021-9258(19)74189-1" @default.
• W1600901894 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8262918" @default.
• W1600901894 hasPublicationYear "1993" @default.
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