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- W1601227390 endingPage "191" @default.
- W1601227390 startingPage "139" @default.
- W1601227390 abstract "This chapter discusses structure and procedure for isolation, and analysis of gangliosides. Most widely used procedure for isolation is based on the ability of gangliosides to partition from the chloroform-methanol phase in which they are extracted into the upper water-enriched phase, leaving behind the bulk of other lipids. There is also a procedure based on DEAE-Sephadex. This method employs an anion-exchange resin to separate gangliosides first, along with other acidic lipids, from the much larger quantity of neutral and zwitterionic lipids that do not bind to the resin. The subsequent steps of base treatment, dialysis, and silicic acid chromatography are designed to remove the other acidic lipids and any acidic low molecular weight contaminants that accompany the gangliosides. Other types of chromatography is used for resolution. Quantitative estimation of gangliosides is usually based on the sialic acid moiety. Various procedures are outlined, along with a gas-liquid chromatographic method that has the attributes, of specificity and sensitivity." @default.
- W1601227390 created "2016-06-24" @default.
- W1601227390 creator A5027791328 @default.
- W1601227390 creator A5046924659 @default.
- W1601227390 date "1982-01-01" @default.
- W1601227390 modified "2023-10-10" @default.
- W1601227390 title "[10] Gangliosides: Structure, isolation, and analysis" @default.
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