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- W1601355957 abstract "Previous observations concerning the ability of the spore-converting bacteriophage PMB12 to cause sporulation in certain sporulation-deficient mutants of Bacillus subtilis 168 were extended to include a spoOK mutant and a mutant temperature sensitive for sporulation due to a ribosomal mutation. Mutants of PMB12 that were unable to induce sporulation in the spoOK mutant were isolated to determine whether PMB12-encoded products had to affect the sporulation-specific functions of both the transcription and the translation systems of B. subtilis to induce sporulation. A complementation assay for spore conversion was used to assign the spore conversion-negative PMB12 mutants to four groups. One group of mutants repressed the ability of wild-type PMB12 to induce sporulation. None of the spore conversion-negative PMB12 mutants could induce significant levels of sporulation in B. subtilis mutants that were temperature sensitive for sporulation due to mutations in the beta subunit of ribonucleic acid polymerase or the 30S ribosomal subunit. Our data suggest that PMB12 may have at least three genes for spore conversion. The products of these genes apparently interact with a host cell pathway that is expressed during the earliest stage of sporulation and is not dependent for expression upon sporulation-specific functions of the host cell's transcription and translation systems." @default.
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- W1601355957 date "1981-03-01" @default.
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- W1601355957 title "Analysis of Bacillus subtilis sporulation with spore-converting bacteriophage PMB12" @default.
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- W1601355957 doi "https://doi.org/10.1128/jb.145.3.1281-1285.1981" @default.
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