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• W1601384787 abstract "Tubulin is a calcium-binding protein. Two different modes of interaction of calcium with tubulin have been described: a high affinity interaction to one or two binding sites and lower affinity interactions to several other binding sites. In the present study, we have used limited proteolysis of tubulin with trypsin, chymotrypsin, and subtilisin to localize the high affinity calcium-binding sites. Our results indicate that two sites are located in the carboxyl-terminal region of both tubulin subunits, and that tubulin deprived of its carboxyl-terminal region is able to polymerize in the presence of 0.5 mM calcium." @default.
• W1601384787 created "2016-06-24" @default.
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• W1601384787 date "1986-05-01" @default.
• W1601384787 modified "2023-10-16" @default.
• W1601384787 title "Localization of the high affinity calcium-binding site on tubulin molecule." @default.
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• W1601384787 doi "https://doi.org/10.1016/s0021-9258(19)62723-7" @default.
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