FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1601403937> ?p ?o ?g. }

• W1601403937 endingPage "217" @default.
• W1601403937 startingPage "209" @default.
• W1601403937 abstract "Purified glycolipids were tested for their ability to serve as acceptors of [14C]fucose from GDP-[14C]fucose as catalyzed by cell-free extracts and purified membrane fractions of human colorectal carcinoma cells, SW1116, cultured in serum-free medium. Purified lactotetraosyl ceramide (Galβ1→3GlcNAcβ1→3Galβ1→4Glc-Cer or LcOse4Cer) and H-1 glycolipid (Fucα1→2Galβ1→3GlcNAcβ1→3Galβ1→4Glc-Cer or IV2 FucαLcOse4Cer) stimulated incorporation of radioactivity into lipid-soluble glycolipid at a rate greater than ten times that of Lea glycolipid [Galβ1→3(Fucα1→4)GlcNAcβ1→3Galβ1→4Glc-Cer or III4 FucαLcOse4Cer]. The enzymatic activities in crude and purified membrane fractions were optimized for substrate concentrations (glycolipid and GDP-fucose), detergent requirement (taurocholate), pH, time and protein. The radioactive product of H-1 fucosylation migrated as discrete and distinct bands on high-performance thin-layer chromatograms (HPTLC). Evidence for their identity with Leb fucolipid described previously [Fucα1→2Galβ1→3(Fucα1→4)GlcNAcβ1→3Galβ1→4Glc-Cer or III4IV2 (Fucα) LcOse4Cer] is presented. The radioactive product of LcOse4Cer fucosylation was mainly Lea fucolipid as determined by co-migration with authentic Lea fucolipid in three HPTLC systems as native and acetylated derivatives. Our results also indicated a low level of H-1 and Leb glycolipid synthesis from LcOse4Cer. On the basis of the optima, linearity for time, and enzyme-limiting conditions, we obtained a 12–19-fold purification of the LcOse4Cer and H-1 fucosyl transferase acceptor activities in three peaks of a sucrose gradient. The peak with the highest specific activity (peak 3) was highest in density and in Na+, K+, ATPase specific activity, although NADH–cytochrome-c reductase and UDP-GalNac transferase were also present in peak 3. The apparent Km values of LcOse4Cer acceptor activity and H-1 acceptor activity in peak 3 were significantly different (p < 0.01) by statistical tests, 2.4 μM and 0.5 μM, respectively. These apparent Km values were much lower (103×) and the pH optima were lower (4.8–5.3), than the corresponding properties reported for the α1→3/α1→4 fucosyl transferase purified from human milk. Our results suggest a role for the non-glycosidic moieties of the acceptors and/or the tissue-specific or primitive expression of these fucosyl transferase activities." @default.
• W1601403937 created "2016-06-24" @default.
• W1601403937 creator A5022316371 @default.
• W1601403937 creator A5075352703 @default.
• W1601403937 date "1987-10-01" @default.
• W1601403937 modified "2023-10-14" @default.
• W1601403937 title "Biosynthesis of Lewis fucolipid antigens in human colorectal carcinoma cells. Partial characterization of LcOse4Cer and H-1 fucolipid fucosyl transferase acceptor activities" @default.
• W1601403937 cites W1000552137 @default.
• W1601403937 cites W1505038125 @default.
• W1601403937 cites W1535809564 @default.
• W1601403937 cites W1539344862 @default.
• W1601403937 cites W1542775374 @default.
• W1601403937 cites W1575885714 @default.
• W1601403937 cites W1585615821 @default.
• W1601403937 cites W1585720781 @default.
• W1601403937 cites W1595870330 @default.
• W1601403937 cites W1599209789 @default.
• W1601403937 cites W1646941904 @default.
• W1601403937 cites W1754937767 @default.
• W1601403937 cites W1848807813 @default.
• W1601403937 cites W1978788736 @default.
• W1601403937 cites W1983080630 @default.
• W1601403937 cites W2026212804 @default.
• W1601403937 cites W2043732014 @default.
• W1601403937 cites W2051322195 @default.
• W1601403937 cites W2059041711 @default.
• W1601403937 cites W2070983900 @default.
• W1601403937 cites W2128635872 @default.
• W1601403937 cites W2151734103 @default.
• W1601403937 cites W2154644112 @default.
• W1601403937 cites W2158343596 @default.
• W1601403937 cites W2169438822 @default.
• W1601403937 cites W2344198552 @default.
• W1601403937 cites W26527402 @default.
• W1601403937 cites W4236056174 @default.
• W1601403937 cites W4247912597 @default.
• W1601403937 doi "https://doi.org/10.1111/j.1432-1033.1987.tb13407.x" @default.
• W1601403937 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3665917" @default.
• W1601403937 hasPublicationYear "1987" @default.
• W1601403937 type Work @default.
• W1601403937 sameAs 1601403937 @default.
• W1601403937 citedByCount "10" @default.
• W1601403937 crossrefType "journal-article" @default.
• W1601403937 hasAuthorship W1601403937A5022316371 @default.
• W1601403937 hasAuthorship W1601403937A5075352703 @default.
• W1601403937 hasBestOaLocation W16014039371 @default.
• W1601403937 hasConcept C108625454 @default.
• W1601403937 hasConcept C181199279 @default.
• W1601403937 hasConcept C185592680 @default.
• W1601403937 hasConcept C22222904 @default.
• W1601403937 hasConcept C2776376580 @default.
• W1601403937 hasConcept C2776841590 @default.
• W1601403937 hasConcept C2777762822 @default.
• W1601403937 hasConcept C2779212266 @default.
• W1601403937 hasConcept C43617362 @default.
• W1601403937 hasConcept C55493867 @default.
• W1601403937 hasConcept C71240020 @default.
• W1601403937 hasConceptScore W1601403937C108625454 @default.
• W1601403937 hasConceptScore W1601403937C181199279 @default.
• W1601403937 hasConceptScore W1601403937C185592680 @default.
• W1601403937 hasConceptScore W1601403937C22222904 @default.
• W1601403937 hasConceptScore W1601403937C2776376580 @default.
• W1601403937 hasConceptScore W1601403937C2776841590 @default.
• W1601403937 hasConceptScore W1601403937C2777762822 @default.
• W1601403937 hasConceptScore W1601403937C2779212266 @default.
• W1601403937 hasConceptScore W1601403937C43617362 @default.
• W1601403937 hasConceptScore W1601403937C55493867 @default.
• W1601403937 hasConceptScore W1601403937C71240020 @default.
• W1601403937 hasIssue "1" @default.
• W1601403937 hasLocation W16014039371 @default.
• W1601403937 hasLocation W16014039372 @default.
• W1601403937 hasOpenAccess W1601403937 @default.
• W1601403937 hasPrimaryLocation W16014039371 @default.
• W1601403937 hasRelatedWork W1601403937 @default.
• W1601403937 hasRelatedWork W1976992722 @default.
• W1601403937 hasRelatedWork W1997825004 @default.
• W1601403937 hasRelatedWork W2005324293 @default.
• W1601403937 hasRelatedWork W2008557803 @default.
• W1601403937 hasRelatedWork W2026212804 @default.
• W1601403937 hasRelatedWork W2038357084 @default.
• W1601403937 hasRelatedWork W2060569376 @default.
• W1601403937 hasRelatedWork W2082505330 @default.
• W1601403937 hasRelatedWork W2132240438 @default.
• W1601403937 hasVolume "168" @default.
• W1601403937 isParatext "false" @default.
• W1601403937 isRetracted "false" @default.
• W1601403937 magId "1601403937" @default.
• W1601403937 workType "article" @default.