FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1601410210> ?p ?o ?g. }

• W1601410210 abstract "Tomato is an economically important crop that can be devastated by many root infecting pathogens. The development of alternative and sustainable crop cultivation techniques and disease control methods is a must for the tomato industry, due to more strict government regulations and concerns over the sustainability of conventional chemical-intensive agriculture (Dixon and Margerison, 2009). In this thesis, the molecular fingerprinting method Terminal-Restriction Fragment Length Polymorphism (T-RFLP) and next generation sequencing method (pyrosequencing) were used, targeting ITS1, ITS2 and 23S ribosomal DNA to characterize and examine microbial community assemblages in the rhizosphere of tomato. These molecular techniques were employed alongside traditional cultivation, microscopy and plant health assessment techniques to determine the effects of growth media, plant age and disease control methods on rhizosphere microbial populations and tomato root health.Plant age and media were found to significantly affect microbial community assemblages; conversely, microbial populations were not altered by soil amendments or rootstock disease control measures used. These findings suggest that the factors influencing rhizosphere community structure can be ranked by importance. Furthermore, if the most influential factors are kept consistent then rhizosphere microbial structures are robust and difficult to perturb with changes in a factor contributing less control over microbial community composition.No direct link between crop health assessments and rhizosphere microbial community diversity or presence of root pathogens could be established. Furthermore, high abundance of potential pathogens and poor crop health assessments during the growing season did not always result in poor health or disease symptoms at the end of cropping assessment in our trials. These results imply that many factors control the rhizosphere competence and ecological role of different species, ultimately affecting the outcome of disease. As no known methods are capable of efficiently assessing the fate of total microorganisms in the rhizosphere over time and space, this study could be considered as part the ‘descriptive phase’ in this field (Kent and Triplett, 2002).Pyrosequencing increased the resolution and confidence of rDNA analysis compared to T-RFLP, identifying organism within samples to a genus and often species level. Advances in next generation sequencing and analytical tools and pipelines associated with this analysis are likely to develop as these methods become common practice. With this in mind, next generation sequencing represents the future approach for resolving complex microbial communities in environmental samples." @default.
• W1601410210 created "2016-06-24" @default.
• W1601410210 creator A5046934680 @default.
• W1601410210 date "2012-12-13" @default.
• W1601410210 modified "2023-09-26" @default.
• W1601410210 title "Monitoring rhizosphere microbial communities of tomato" @default.
• W1601410210 cites W114114092 @default.
• W1601410210 cites W1483247593 @default.
• W1601410210 cites W1490372903 @default.
• W1601410210 cites W1498625990 @default.
• W1601410210 cites W1530067309 @default.
• W1601410210 cites W1535624277 @default.
• W1601410210 cites W1540563425 @default.
• W1601410210 cites W1558982506 @default.
• W1601410210 cites W1582959026 @default.
• W1601410210 cites W1588533534 @default.
• W1601410210 cites W1600975622 @default.
• W1601410210 cites W1768317793 @default.
• W1601410210 cites W1770972723 @default.
• W1601410210 cites W1819781817 @default.
• W1601410210 cites W1878193911 @default.
• W1601410210 cites W1913861260 @default.
• W1601410210 cites W1922258314 @default.
• W1601410210 cites W1963176932 @default.
• W1601410210 cites W1966000702 @default.
• W1601410210 cites W1966904937 @default.
• W1601410210 cites W1967423469 @default.
• W1601410210 cites W1968569710 @default.
• W1601410210 cites W1970593500 @default.
• W1601410210 cites W1972155404 @default.
• W1601410210 cites W1973818983 @default.
• W1601410210 cites W1974535877 @default.
• W1601410210 cites W1974551185 @default.
• W1601410210 cites W1977882896 @default.
• W1601410210 cites W1978253706 @default.
• W1601410210 cites W1979845410 @default.
• W1601410210 cites W1984224973 @default.
• W1601410210 cites W1988660274 @default.
• W1601410210 cites W1991132473 @default.
• W1601410210 cites W1992177841 @default.
• W1601410210 cites W1995875735 @default.
• W1601410210 cites W1996081325 @default.
• W1601410210 cites W1998602558 @default.
• W1601410210 cites W2000873273 @default.
• W1601410210 cites W2001233818 @default.
• W1601410210 cites W2002504695 @default.
• W1601410210 cites W2002861821 @default.
• W1601410210 cites W2002985470 @default.
• W1601410210 cites W2003485756 @default.
• W1601410210 cites W2004646076 @default.
• W1601410210 cites W2008067742 @default.
• W1601410210 cites W2008693216 @default.
• W1601410210 cites W2009770591 @default.
• W1601410210 cites W2011255680 @default.
• W1601410210 cites W2011824484 @default.
• W1601410210 cites W2011927316 @default.
• W1601410210 cites W2014170469 @default.
• W1601410210 cites W2017435247 @default.
• W1601410210 cites W202119149 @default.
• W1601410210 cites W2021258458 @default.
• W1601410210 cites W2027188586 @default.
• W1601410210 cites W2027583306 @default.
• W1601410210 cites W2027866856 @default.
• W1601410210 cites W2028816874 @default.
• W1601410210 cites W2029028335 @default.
• W1601410210 cites W2030966943 @default.
• W1601410210 cites W2031857899 @default.
• W1601410210 cites W2034056649 @default.
• W1601410210 cites W2034469347 @default.
• W1601410210 cites W2034762419 @default.
• W1601410210 cites W2034941720 @default.
• W1601410210 cites W2041726221 @default.
• W1601410210 cites W2044002913 @default.
• W1601410210 cites W2045762639 @default.
• W1601410210 cites W2047249932 @default.
• W1601410210 cites W2047790688 @default.
• W1601410210 cites W2048464756 @default.
• W1601410210 cites W2049392778 @default.
• W1601410210 cites W2049941170 @default.
• W1601410210 cites W2054908644 @default.
• W1601410210 cites W2055043387 @default.
• W1601410210 cites W2055492095 @default.
• W1601410210 cites W2056147936 @default.
• W1601410210 cites W2058540779 @default.
• W1601410210 cites W2061177996 @default.
• W1601410210 cites W2061245530 @default.
• W1601410210 cites W2063787532 @default.
• W1601410210 cites W2064859201 @default.
• W1601410210 cites W2066432447 @default.
• W1601410210 cites W2066782710 @default.
• W1601410210 cites W2067161026 @default.
• W1601410210 cites W2069160087 @default.
• W1601410210 cites W2070553419 @default.
• W1601410210 cites W2072232920 @default.
• W1601410210 cites W2077424838 @default.
• W1601410210 cites W2077989480 @default.
• W1601410210 cites W2078088066 @default.
• W1601410210 cites W2078886175 @default.
• W1601410210 cites W2082370994 @default.
• W1601410210 cites W2085770507 @default.

• next