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• W1601703854 abstract "This chapter describes how a confocal microscope can be treated as a spectrophotometer to measure the absolute number of fluorescent molecules in live cells (Wu and Pollard, 2005).(1) The method provides dynamic range of over three orders of magnitude for counting the number of molecules in a single cell. We present a step-by-step guide to measure concentrations in vivo, explaining many of the practical considerations for using this technique. This chapter is meant as a resource for cell biologists, biochemists, and biophysicists interested in quantifying macromolecules involved in their favorite molecular pathways in live cells." @default.
• W1601703854 created "2016-06-24" @default.
• W1601703854 creator A5043478405 @default.
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• W1601703854 date "2008-01-01" @default.
• W1601703854 modified "2023-10-13" @default.
• W1601703854 title "Chapter 9 Counting Proteins in Living Cells by Quantitative Fluorescence Microscopy with Internal Standards" @default.
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• W1601703854 doi "https://doi.org/10.1016/s0091-679x(08)00609-2" @default.
• W1601703854 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19118678" @default.
• W1601703854 hasPublicationYear "2008" @default.
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